Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU46_RS01940 Genome accession   NZ_AP024866
Coordinates   400067..401170 (+) Length   367 a.a.
NCBI ID   WP_141273165.1    Uniprot ID   A0A4Y3ITG7
Organism   Vibrio comitans strain LMG 23416     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395067..406170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU46_RS01910 - 395734..396339 (-) 606 WP_141273153.1 XTP/dITP diphosphatase -
  OCU46_RS01915 - 396344..396787 (-) 444 WP_141273155.1 DUF4426 domain-containing protein -
  OCU46_RS01920 - 396815..397384 (-) 570 WP_141273157.1 YggT family protein -
  OCU46_RS01925 proC 397403..398227 (-) 825 WP_141273159.1 pyrroline-5-carboxylate reductase -
  OCU46_RS01930 - 398256..398966 (-) 711 WP_141273161.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU46_RS01935 pilT 398991..400037 (+) 1047 WP_141273163.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU46_RS01940 pilU 400067..401170 (+) 1104 WP_141273165.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU46_RS01945 ruvX 401174..401599 (-) 426 WP_141273167.1 Holliday junction resolvase RuvX -
  OCU46_RS01950 - 401596..402156 (-) 561 WP_141273169.1 YqgE/AlgH family protein -
  OCU46_RS01955 gshB 402224..403171 (-) 948 WP_141273171.1 glutathione synthase -
  OCU46_RS01960 rsmE 403181..403912 (-) 732 WP_141273173.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU46_RS01965 - 404007..404663 (-) 657 WP_167495437.1 endonuclease -
  OCU46_RS01970 - 404822..405316 (-) 495 WP_141273177.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40869.61 Da        Isoelectric Point: 5.7234

>NTDB_id=88917 OCU46_RS01940 WP_141273165.1 400067..401170(+) (pilU) [Vibrio comitans strain LMG 23416]
MELKELLDAMIEHKASDLYITVDSPCMFRVDGELQPFGEKLHQSGVTAILDSIMDNDRRKEYRAIREANFAIESESGRFR
VSAFFQRDLPGAVIRHIETNIPSFDELGLPDTLKDLAMVKRGLVLVVGATGSGKSTTLAAMVGHRNQHQRGHILTVEDPI
EFVHQHQNSIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTPETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPQAQREHFLLDLSLNLKGIVGQQLLKAKDDSGRHIALEILLVTPRASDLVRQGEIAEIKNYMAKTKQEGICTFDQSLF
DLVVQGKVSEEEALRSADSANDLRLMLKNHQGTSSSGPLDDIKIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=88917 OCU46_RS01940 WP_141273165.1 400067..401170(+) (pilU) [Vibrio comitans strain LMG 23416]
ATGGAATTAAAAGAACTGCTAGATGCGATGATAGAGCATAAGGCATCGGATCTGTATATCACCGTAGATTCTCCTTGTAT
GTTTCGAGTTGATGGAGAGCTTCAGCCTTTTGGGGAAAAGCTACATCAGTCAGGGGTGACCGCTATTCTTGATAGCATCA
TGGATAACGATAGGCGCAAAGAATATCGCGCCATTCGAGAGGCAAACTTTGCCATAGAAAGTGAATCAGGACGCTTTAGG
GTCAGTGCTTTCTTTCAGCGAGATCTTCCCGGTGCCGTTATTCGTCATATTGAAACCAATATTCCGTCTTTTGATGAGCT
TGGCTTGCCTGATACGTTGAAAGATCTCGCTATGGTAAAGCGTGGCTTAGTGCTGGTGGTTGGTGCTACGGGATCAGGAA
AATCCACAACTTTGGCTGCTATGGTCGGGCATAGAAATCAACACCAGCGAGGGCATATTCTTACGGTAGAAGACCCTATA
GAGTTTGTGCATCAACATCAAAATAGCATAGTCACGCAGCGTGAGGTTGGGTTAGATACCGAGAGCTATGAAATTGCACT
AAAGAACTCTCTACGACAAGCGCCAGATATGATTGTCATTGGGGAGATTCGAACGCCTGAAACTATGCGTTATGCCATGA
CCTTTGCGGAAACTGGTCACCTGTGCGTTGCAACCTTGCATGCAAACAATGCTAATCAAGCACTTGAACGGATTTTACAT
TTAGTCCCCCAAGCTCAACGAGAGCACTTTTTATTAGACCTCTCTCTTAACCTGAAAGGGATCGTAGGTCAGCAATTGCT
GAAAGCAAAAGATGATAGTGGGCGTCATATTGCACTAGAGATCTTATTGGTGACACCTAGGGCTTCGGATCTTGTTCGTC
AGGGAGAGATCGCAGAGATCAAGAATTACATGGCGAAGACCAAGCAAGAAGGAATATGTACCTTTGATCAATCGCTGTTT
GATTTAGTGGTGCAGGGTAAGGTCAGTGAGGAAGAGGCTTTGCGAAGCGCAGATTCTGCAAATGACCTGCGCTTGATGCT
AAAAAATCATCAGGGGACGAGCAGTAGTGGCCCGTTAGATGATATTAAAATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y3ITG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.665

100

0.687
  pilU Pseudomonas stutzeri DSM 10701

55.429

95.368

0.529
  pilU Acinetobacter baylyi ADP1

53.022

99.183

0.526
  pilT Legionella pneumophila strain Lp02

43.658

92.371

0.403
  pilT Legionella pneumophila strain ERS1305867

43.658

92.371

0.403
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.402

100

0.395
  pilT Acinetobacter baylyi ADP1

40.176

92.916

0.373
  pilT Neisseria gonorrhoeae MS11

39.71

94.005

0.373
  pilT Neisseria meningitidis 8013

39.71

94.005

0.373
  pilT Acinetobacter baumannii strain A118

40.118

92.371

0.371
  pilT Acinetobacter baumannii D1279779

40.118

92.371

0.371
  pilT Acinetobacter nosocomialis M2

40.118

92.371

0.371
  pilT Pseudomonas aeruginosa PAK

39.823

92.371

0.368
  pilT Vibrio cholerae strain A1552

40.419

91.008

0.368
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

91.008

0.368

Multiple sequence alignment