Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU35_RS02130 Genome accession   NZ_AP024864
Coordinates   439530..440633 (+) Length   367 a.a.
NCBI ID   WP_017027145.1    Uniprot ID   A0AAJ3S9U2
Organism   Vibrio breoganii strain CAIM 1829     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 434530..445633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU35_RS02100 - 435194..435799 (-) 606 WP_261825572.1 XTP/dITP diphosphatase -
  OCU35_RS02105 - 435804..436247 (-) 444 WP_017032834.1 DUF4426 domain-containing protein -
  OCU35_RS02110 - 436278..436847 (-) 570 WP_017027141.1 YggT family protein -
  OCU35_RS02115 proC 436866..437690 (-) 825 WP_083188988.1 pyrroline-5-carboxylate reductase -
  OCU35_RS02120 - 437719..438429 (-) 711 WP_261825573.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU35_RS02125 pilT 438454..439500 (+) 1047 WP_065599650.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU35_RS02130 pilU 439530..440633 (+) 1104 WP_017027145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU35_RS02135 ruvX 440637..441062 (-) 426 WP_017027146.1 Holliday junction resolvase RuvX -
  OCU35_RS02140 - 441059..441619 (-) 561 WP_261825574.1 YqgE/AlgH family protein -
  OCU35_RS02145 gshB 441687..442634 (-) 948 WP_017032827.1 glutathione synthase -
  OCU35_RS02150 rsmE 442644..443375 (-) 732 WP_017032826.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU35_RS02155 - 443470..444165 (-) 696 WP_390208390.1 endonuclease -
  OCU35_RS02160 - 444301..444795 (-) 495 WP_065599653.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40872.53 Da        Isoelectric Point: 5.8748

>NTDB_id=88887 OCU35_RS02130 WP_017027145.1 439530..440633(+) (pilU) [Vibrio breoganii strain CAIM 1829]
MELKELLDAMTEHKASDLYITVDSPCMFRVDGELQPFGEKLHQSGVTAILDSIMDNDRRKEYRAIREANFAIESESGRFR
VSAFFQRDLPGAVIRHIETNIPSFDELGLPDTLKDLAMVKRGLVLVVGATGSGKSTTLAAMVGHRNQHQRGHILTVEDPI
EFVHQHQKSIVTQREVGLDTESYEIALKNSLRQAPDMIVIGEIRTPETMRYAMTFAETGHLCVATLHANNANQALERILH
LVPQGQREHFLLDLSLNLKGIVGQQLLKAQDDSGRHAALEILLVTPRASDLIRQGEIAEIKNYMSKTKQQGICTFDQSLF
DLVVQGRVSEEEALRSADSANDLRLMLKNHQGTSSSGPLDDIKIDMG

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=88887 OCU35_RS02130 WP_017027145.1 439530..440633(+) (pilU) [Vibrio breoganii strain CAIM 1829]
ATGGAATTAAAAGAACTGTTAGATGCGATGACAGAGCATAAGGCATCGGATCTGTATATCACTGTAGATTCTCCTTGTAT
GTTTCGAGTGGACGGTGAGCTTCAGCCTTTTGGCGAAAAACTGCATCAATCTGGAGTAACCGCCATCCTTGATAGCATCA
TGGACAACGATAGGCGTAAAGAATATCGAGCCATTAGGGAAGCAAACTTTGCCATAGAAAGTGAATCGGGACGCTTTAGG
GTCAGTGCTTTCTTTCAGAGAGATCTTCCAGGCGCTGTCATTCGTCATATTGAAACCAACATTCCTTCTTTTGATGAGCT
TGGCTTACCGGATACGCTAAAAGATCTCGCAATGGTCAAGCGCGGTTTAGTGCTGGTGGTTGGCGCGACGGGCTCAGGTA
AATCGACGACGTTAGCCGCTATGGTGGGACATAGAAATCAACACCAACGAGGGCATATTCTTACGGTAGAAGACCCTATC
GAGTTTGTGCATCAGCACCAAAAGAGCATAGTCACTCAGCGTGAGGTTGGCTTAGATACTGAGAGCTATGAAATTGCCTT
AAAGAACTCTCTAAGACAAGCTCCAGATATGATCGTCATTGGTGAGATTCGAACACCAGAAACCATGCGTTATGCGATGA
CCTTTGCGGAGACGGGGCACTTGTGTGTTGCGACCTTGCATGCAAACAATGCCAACCAAGCACTAGAACGCATTTTACAC
TTAGTTCCTCAAGGTCAGCGAGAGCACTTTTTACTCGATCTCTCTCTTAACCTGAAAGGGATCGTAGGTCAGCAACTGCT
TAAGGCACAAGATGATAGCGGACGCCATGCCGCACTAGAGATCTTATTGGTGACGCCGAGAGCGTCGGATCTTATTCGTC
AGGGAGAGATCGCAGAGATCAAGAACTACATGTCTAAGACTAAGCAGCAGGGGATCTGTACCTTTGATCAATCACTCTTT
GATTTAGTCGTGCAGGGTAGGGTGAGTGAGGAAGAGGCTTTGCGAAGCGCAGACTCTGCAAACGATCTGCGCTTAATGCT
TAAGAATCATCAAGGGACTAGCAGTAGCGGTCCGTTAGATGATATTAAAATTGATATGGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.12

100

0.681
  pilU Pseudomonas stutzeri DSM 10701

55.714

95.368

0.531
  pilU Acinetobacter baylyi ADP1

53.297

99.183

0.529
  pilT Legionella pneumophila strain ERS1305867

44.248

92.371

0.409
  pilT Legionella pneumophila strain Lp02

44.248

92.371

0.409
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.402

100

0.395
  pilT Neisseria gonorrhoeae MS11

40.87

94.005

0.384
  pilT Neisseria meningitidis 8013

40.87

94.005

0.384
  pilT Acinetobacter baylyi ADP1

40.762

92.916

0.379
  pilT Acinetobacter baumannii strain A118

40.708

92.371

0.376
  pilT Acinetobacter baumannii D1279779

40.708

92.371

0.376
  pilT Acinetobacter nosocomialis M2

40.708

92.371

0.376
  pilT Vibrio cholerae strain A1552

41.317

91.008

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

91.008

0.376
  pilT Pseudomonas aeruginosa PAK

40.413

92.371

0.373
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.371

0.362

Multiple sequence alignment