Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU35_RS02125 Genome accession   NZ_AP024864
Coordinates   438454..439500 (+) Length   348 a.a.
NCBI ID   WP_065599650.1    Uniprot ID   -
Organism   Vibrio breoganii strain CAIM 1829     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 433454..444500
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU35_RS02095 hemW 434019..435197 (-) 1179 WP_261825571.1 radical SAM family heme chaperone HemW -
  OCU35_RS02100 - 435194..435799 (-) 606 WP_261825572.1 XTP/dITP diphosphatase -
  OCU35_RS02105 - 435804..436247 (-) 444 WP_017032834.1 DUF4426 domain-containing protein -
  OCU35_RS02110 - 436278..436847 (-) 570 WP_017027141.1 YggT family protein -
  OCU35_RS02115 proC 436866..437690 (-) 825 WP_083188988.1 pyrroline-5-carboxylate reductase -
  OCU35_RS02120 - 437719..438429 (-) 711 WP_261825573.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU35_RS02125 pilT 438454..439500 (+) 1047 WP_065599650.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU35_RS02130 pilU 439530..440633 (+) 1104 WP_017027145.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU35_RS02135 ruvX 440637..441062 (-) 426 WP_017027146.1 Holliday junction resolvase RuvX -
  OCU35_RS02140 - 441059..441619 (-) 561 WP_261825574.1 YqgE/AlgH family protein -
  OCU35_RS02145 gshB 441687..442634 (-) 948 WP_017032827.1 glutathione synthase -
  OCU35_RS02150 rsmE 442644..443375 (-) 732 WP_017032826.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU35_RS02155 - 443470..444165 (-) 696 WP_390208390.1 endonuclease -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38625.12 Da        Isoelectric Point: 5.9314

>NTDB_id=88886 OCU35_RS02125 WP_065599650.1 438454..439500(+) (pilT) [Vibrio breoganii strain CAIM 1829]
MDITELLRFSVKHNASDLHLSSGLPPMVRIDGDVRRLEMPEFSEAEVHELIFDIMDDAQRSEFESNLELDFSIELGGVGR
FRVNAFNQSRGASAVFRTIPTTIPTLEQLDCPPIFEKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINRHHNKHILTIED
PIEFVHQSSKCLINQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGDKNMVRSMLSESLRAVIAQKLLKRIGGGRVACHELMLANPAIRNLIREDKVAQMYSIIQTGAAQGMRTMEQSV
NQLMQQNLVSREEAEAKVEIQPSLGSGF

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=88886 OCU35_RS02125 WP_065599650.1 438454..439500(+) (pilT) [Vibrio breoganii strain CAIM 1829]
ATGGATATCACCGAATTGCTTCGCTTTAGTGTAAAGCATAATGCCTCAGATCTACATCTTTCATCGGGACTACCGCCAAT
GGTAAGGATTGATGGTGATGTGAGACGCTTGGAAATGCCTGAATTTAGTGAAGCTGAGGTACATGAACTTATCTTCGATA
TTATGGATGATGCGCAGCGAAGTGAGTTTGAATCTAACCTTGAGTTGGATTTTTCCATTGAGCTGGGTGGAGTGGGTCGA
TTTAGGGTTAATGCTTTTAATCAAAGCCGTGGAGCCTCTGCGGTGTTTCGAACCATTCCCACTACGATTCCTACCCTTGA
GCAACTTGATTGCCCACCTATATTTGAAAAAATAGCGAATTATGAAAAGGGATTGGTTTTGGTGACTGGGCCTACGGGGT
CGGGTAAATCAACCACCTTAGCGGCCATGGTTGATTACATTAATCGTCATCACAACAAACACATCTTAACCATTGAAGAT
CCCATTGAGTTTGTTCATCAAAGCTCTAAGTGTTTGATCAACCAAAGGGAAGTGCATCGTGATACGCATAGCTTCAAGGC
TGCGCTTCGTTCTGCACTGCGTGAAGACCCTGATGTGATTCTAGTGGGTGAGCTTCGAGACCAAGAAACCATAAGCCTTG
CGCTTACTGCAGCGGAAACGGGGCATTTGGTCTTTGGTACCCTGCACACCAGTTCTGCGGCCAAAACCATAGACCGTATT
ATTGATGTATTCCCTGCGGGCGATAAAAACATGGTTCGCTCTATGTTGTCAGAGTCATTGCGCGCTGTTATCGCGCAAAA
ATTGCTAAAGCGTATTGGCGGAGGCCGAGTAGCCTGTCATGAGCTCATGTTAGCGAACCCAGCTATTCGCAACCTTATTC
GAGAGGATAAGGTGGCGCAGATGTATTCCATTATCCAAACTGGCGCGGCTCAAGGCATGAGAACTATGGAGCAATCGGTA
AATCAATTGATGCAACAAAATCTGGTTAGCCGCGAAGAAGCAGAGGCTAAGGTTGAGATACAGCCTAGCTTAGGTTCTGG
CTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

82.059

97.701

0.802
  pilT Vibrio cholerae strain A1552

82.059

97.701

0.802
  pilT Pseudomonas stutzeri DSM 10701

69.298

98.276

0.681
  pilT Acinetobacter nosocomialis M2

68.421

98.276

0.672
  pilT Acinetobacter baumannii D1279779

68.421

98.276

0.672
  pilT Acinetobacter baumannii strain A118

68.421

98.276

0.672
  pilT Acinetobacter baylyi ADP1

69.345

96.552

0.67
  pilT Pseudomonas aeruginosa PAK

68.529

97.701

0.67
  pilT Legionella pneumophila strain Lp02

67.066

95.977

0.644
  pilT Legionella pneumophila strain ERS1305867

67.066

95.977

0.644
  pilT Neisseria meningitidis 8013

64.638

99.138

0.641
  pilT Neisseria gonorrhoeae MS11

64.348

99.138

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.343

96.264

0.494
  pilU Pseudomonas stutzeri DSM 10701

42.09

96.264

0.405
  pilU Vibrio cholerae strain A1552

41.791

96.264

0.402
  pilU Acinetobacter baylyi ADP1

38.988

96.552

0.376

Multiple sequence alignment