Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OC457_RS02215 Genome accession   NZ_AP024854
Coordinates   437308..438345 (+) Length   345 a.a.
NCBI ID   WP_080174541.1    Uniprot ID   A0A1T4SQK7
Organism   Photobacterium toruni strain CECT 9189     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 432308..443345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OC457_RS02180 - 432367..432711 (+) 345 WP_159447852.1 DUF2884 family protein -
  OC457_RS02185 hemW 432830..433990 (-) 1161 WP_080174537.1 radical SAM family heme chaperone HemW -
  OC457_RS02190 - 433994..434584 (-) 591 WP_080174560.1 XTP/dITP diphosphatase -
  OC457_RS02195 yggU 434795..435091 (-) 297 WP_080174538.1 DUF167 family protein YggU -
  OC457_RS02200 - 435088..435645 (-) 558 WP_045037796.1 YggT family protein -
  OC457_RS02205 proC 435655..436473 (-) 819 WP_080174539.1 pyrroline-5-carboxylate reductase -
  OC457_RS02210 - 436572..437282 (-) 711 WP_080174540.1 YggS family pyridoxal phosphate-dependent enzyme -
  OC457_RS02215 pilT 437308..438345 (+) 1038 WP_080174541.1 type IV pilus twitching motility protein PilT Machinery gene
  OC457_RS02220 pilU 438362..439468 (+) 1107 WP_080174542.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OC457_RS02225 ruvX 439826..440257 (-) 432 WP_080174543.1 Holliday junction resolvase RuvX -
  OC457_RS02230 - 440254..440817 (-) 564 WP_080174544.1 YqgE/AlgH family protein -
  OC457_RS02235 gshB 440938..441888 (-) 951 WP_080174545.1 glutathione synthase -
  OC457_RS02240 rsmE 441906..442637 (-) 732 WP_080174546.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38139.64 Da        Isoelectric Point: 6.1266

>NTDB_id=88835 OC457_RS02215 WP_080174541.1 437308..438345(+) (pilT) [Photobacterium toruni strain CECT 9189]
MDITELLDFSVKHNASDLHLSAGVPPIIRVDGDVRKLSLPALTHTEVHQLIFDIMNDAQRREYEEKLEVDFSFELPEVGR
FRVNAFHQSRGASAVLRTIPMNIPTLASLNVPEVFYDIAQLNRGLVLVTGATGSGKSTTIAALVDYINSNYQRHILTIED
PIEFVHTSKSCLINQREVHRDTLSFQNALRSSLREDPDVIVVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTVDRI
IDVFPGNDKSMVRSMLSESLRAVVAQNLLKCTSGGRVACHEVMMATPAIRNLIREDKIAQMYSMIQTGSSLGMQTMEQSV
KMLVAQGMVAAEEGRRILDSNQRGF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=88835 OC457_RS02215 WP_080174541.1 437308..438345(+) (pilT) [Photobacterium toruni strain CECT 9189]
ATGGATATTACCGAATTACTCGATTTTAGTGTAAAGCATAACGCATCAGATCTGCACCTTTCTGCGGGTGTTCCACCAAT
AATAAGGGTGGATGGTGATGTGAGAAAGCTCAGTTTACCTGCTTTAACTCATACAGAAGTGCACCAATTGATCTTTGATA
TTATGAACGATGCACAGCGTCGAGAATATGAAGAAAAATTAGAAGTCGATTTTTCTTTTGAATTACCTGAAGTTGGTCGT
TTCAGGGTTAATGCTTTTCATCAGTCTCGTGGTGCTTCTGCTGTACTACGAACTATCCCTATGAATATTCCTACGTTAGC
GTCACTGAATGTTCCTGAGGTTTTTTATGATATTGCGCAGCTTAACCGTGGGTTAGTACTGGTCACTGGCGCAACGGGCT
CAGGTAAATCAACCACCATTGCCGCCTTAGTAGATTACATCAATAGCAACTATCAACGCCATATATTGACCATTGAAGAT
CCGATCGAATTTGTCCATACCAGTAAAAGCTGTCTGATCAATCAACGTGAAGTGCATCGCGATACCTTAAGTTTCCAAAA
TGCACTGCGATCATCATTGCGTGAAGATCCCGATGTGATTGTCGTGGGTGAGTTGCGTGATCAAGAAACCATTAGTTTGG
CATTAACGGCAGCTGAAACCGGTCACTTGGTATTAGGTACTTTGCATACCAGTTCAGCAGCTAAAACGGTTGACCGTATT
ATTGATGTTTTTCCTGGTAATGATAAATCGATGGTGCGCTCAATGTTGTCAGAATCATTGCGCGCCGTTGTGGCACAAAA
CTTATTGAAGTGTACCTCTGGTGGTCGTGTTGCGTGTCATGAAGTGATGATGGCAACGCCGGCTATTCGTAATTTAATCC
GTGAAGATAAAATTGCTCAGATGTATTCGATGATTCAAACCGGTTCATCACTTGGTATGCAGACCATGGAACAAAGTGTA
AAAATGCTGGTGGCACAAGGAATGGTAGCTGCCGAAGAAGGGCGTCGTATTCTTGATAGTAATCAACGTGGATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1T4SQK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.652

100

0.757
  pilT Vibrio cholerae strain A1552

75.652

100

0.757
  pilT Acinetobacter baylyi ADP1

67.544

99.13

0.67
  pilT Acinetobacter baumannii strain A118

68.955

97.101

0.67
  pilT Acinetobacter baumannii D1279779

68.955

97.101

0.67
  pilT Pseudomonas stutzeri DSM 10701

68.657

97.101

0.667
  pilT Pseudomonas aeruginosa PAK

68.657

97.101

0.667
  pilT Acinetobacter nosocomialis M2

68.657

97.101

0.667
  pilT Legionella pneumophila strain Lp02

68.485

95.652

0.655
  pilT Legionella pneumophila strain ERS1305867

68.485

95.652

0.655
  pilT Neisseria meningitidis 8013

64.095

97.681

0.626
  pilT Neisseria gonorrhoeae MS11

63.798

97.681

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.547

99.71

0.484
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.349

98.841

0.409
  pilU Acinetobacter baylyi ADP1

40.062

93.333

0.374

Multiple sequence alignment