Detailed information NTG0088836

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OC457_RS02220
• Genome accession: NZ_AP024854
• Coordinates: 438362..439468 (+)
• Length: 368 a.a.
• NCBI ID: WP_080174542.1
• Uniprot ID: -
• Organism: Photobacterium toruni strain CECT 9189
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433362..444468

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OC457_RS02190	-	433994..434584 (-)	591	WP_080174560.1	XTP/dITP diphosphatase	-
OC457_RS02195	yggU	434795..435091 (-)	297	WP_080174538.1	DUF167 family protein YggU	-
OC457_RS02200	-	435088..435645 (-)	558	WP_045037796.1	YggT family protein	-
OC457_RS02205	proC	435655..436473 (-)	819	WP_080174539.1	pyrroline-5-carboxylate reductase	-
OC457_RS02210	-	436572..437282 (-)	711	WP_080174540.1	YggS family pyridoxal phosphate-dependent enzyme	-
OC457_RS02215	pilT	437308..438345 (+)	1038	WP_080174541.1	type IV pilus twitching motility protein PilT	Machinery gene
OC457_RS02220	pilU	438362..439468 (+)	1107	WP_080174542.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OC457_RS02225	ruvX	439826..440257 (-)	432	WP_080174543.1	Holliday junction resolvase RuvX	-
OC457_RS02230	-	440254..440817 (-)	564	WP_080174544.1	YqgE/AlgH family protein	-
OC457_RS02235	gshB	440938..441888 (-)	951	WP_080174545.1	glutathione synthase	-
OC457_RS02240	rsmE	441906..442637 (-)	732	WP_080174546.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
OC457_RS02245	-	442871..443581 (-)	711	WP_080174547.1	endonuclease	-
OC457_RS02250	-	443705..444196 (-)	492	WP_080174548.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 41276.25 Da
• Isoelectric Point: 5.5848

>NTDB_id=88836 OC457_RS02220 WP_080174542.1 438362..439468(+) (pilU) [Photobacterium toruni strain CECT 9189]
MTLDDILEQVVIKKASDLYITVDSACLLKIDGNLHDIGKVLTRDDVDSLFDQAMDANLRQEFISTKEANFALVRNEHRFR
VSAFWQRELPGMVIRRIESQIPQISDLQLPIEMERLALSKRGLVLIVGATGSGKSTSMAAMTGYRNQHRSGHILTVEDPI
EFVHKHNKCIVTQREVGLDTASYEVALKNSLRQAPDMILIGEIRTRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPEERRDQFLFDLSLNLKCILAQQLVADANGVGRHGVYELLLNTPRVADLIRRGDLYEIKDVMAKSTDAGMITFDQSLY
ALFSEGKITEQDALYHADSANDLRLMIKLGDHSIQPLSSLSGVTLENS

Nucleotide

• Length: 1107 bp

>NTDB_id=88836 OC457_RS02220 WP_080174542.1 438362..439468(+) (pilU) [Photobacterium toruni strain CECT 9189]
ATGACGTTAGATGACATTCTTGAACAGGTTGTTATTAAAAAAGCTTCTGATTTATATATTACGGTTGATTCAGCGTGTTT
ATTAAAAATTGACGGTAATCTGCATGATATAGGCAAGGTATTAACCCGTGATGATGTTGATAGTTTGTTTGATCAAGCGA
TGGATGCCAACCTCAGACAGGAGTTTATCAGTACCAAAGAAGCTAATTTTGCTTTAGTGCGTAATGAACATCGATTTCGT
GTCAGTGCTTTTTGGCAACGAGAATTACCCGGAATGGTGATCCGTCGTATTGAATCTCAAATTCCGCAAATAAGTGACTT
GCAGTTACCGATTGAGATGGAGCGATTAGCATTATCGAAACGGGGTTTGGTATTAATTGTCGGGGCGACAGGTTCTGGTA
AATCAACCTCAATGGCAGCCATGACGGGCTATCGTAATCAGCATCGAAGTGGGCATATTTTAACGGTTGAAGATCCAATT
GAATTTGTTCATAAACACAACAAATGCATTGTCACTCAGCGTGAAGTTGGGCTGGACACTGCTAGCTATGAAGTGGCACT
AAAAAATTCATTACGCCAAGCACCAGATATGATTTTGATTGGTGAAATTCGTACTCGTGAAACGATGGAATATGCGATGA
ATTTTGCTGAAACTGGCCATTTATGTATGGCAACGTTGCATGCCAATAATGCTAATCAGGCATTAGAGCGAATTTTACAT
TTAGTACCTGAAGAACGACGTGATCAATTTTTATTTGATTTATCTCTTAATCTTAAATGTATTTTGGCGCAGCAGTTAGT
TGCTGATGCTAATGGTGTTGGTCGCCATGGTGTGTATGAATTATTACTGAATACACCGCGAGTTGCCGATTTAATTCGAC
GGGGTGATTTGTATGAAATTAAAGATGTGATGGCGAAATCAACGGATGCAGGAATGATAACGTTTGACCAGTCATTGTAT
GCGTTATTTAGCGAAGGAAAAATAACTGAGCAAGATGCGCTGTACCATGCCGACTCCGCTAATGATTTACGGTTAATGAT
TAAATTGGGTGATCATAGTATTCAGCCGTTAAGTAGTTTATCGGGAGTAACGCTTGAAAACAGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (66-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Vibrio cholerae strain A1552	70.115	94.565	0.663
pilU	Pseudomonas stutzeri DSM 10701	57.979	100	0.592
pilU	Acinetobacter baylyi ADP1	54.42	98.37	0.535
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.653	91.576	0.372