Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OCU77_RS02465 Genome accession   NZ_AP024852
Coordinates   500444..501481 (+) Length   345 a.a.
NCBI ID   WP_048901013.1    Uniprot ID   A0A0J8V4Q0
Organism   Photobacterium swingsii strain CECT 7576     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 495444..506481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU77_RS02435 - 496542..497135 (-) 594 WP_048901008.1 XTP/dITP diphosphatase -
  OCU77_RS02440 - 497330..497758 (-) 429 WP_048901009.1 DUF4426 domain-containing protein -
  OCU77_RS02445 yggU 497925..498224 (-) 300 WP_048901010.1 DUF167 family protein YggU -
  OCU77_RS02450 - 498221..498778 (-) 558 WP_107303068.1 YggT family protein -
  OCU77_RS02455 proC 498788..499606 (-) 819 WP_048901011.1 pyrroline-5-carboxylate reductase -
  OCU77_RS02460 - 499710..500420 (-) 711 WP_048901012.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU77_RS02465 pilT 500444..501481 (+) 1038 WP_048901013.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU77_RS02470 pilU 501491..502597 (+) 1107 WP_048901014.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU77_RS02475 ruvX 502984..503415 (-) 432 WP_048901015.1 Holliday junction resolvase RuvX -
  OCU77_RS02480 - 503412..503975 (-) 564 WP_048901016.1 YqgE/AlgH family protein -
  OCU77_RS02485 gshB 504172..505119 (-) 948 WP_048901017.1 glutathione synthase -
  OCU77_RS02490 rsmE 505136..505867 (-) 732 WP_048901018.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38198.61 Da        Isoelectric Point: 6.3173

>NTDB_id=88799 OCU77_RS02465 WP_048901013.1 500444..501481(+) (pilT) [Photobacterium swingsii strain CECT 7576]
MDITELLDFSVKHNASDLHLSAGVPPIIRVDGDVRKLSLPALAHSEVHRLVFDIMNDAQRREYEEKLEVDFSFELPDVGR
FRVNAFHQSRGSAAVFRTIPMNVPTLESLKVPDIFYDIAQLPRGLVLVTGATGSGKSTTIAALVDYINENYNRHILTIED
PVEFVHHSKRSLINQREVHRDTHSFQAALRSSLREDPDVILVGELRDQETISLALTAAETGHLVLGTLHTSSAAKTIDRI
IDVFPGNDKSMVRSMLSESLRAVVSQSLLKCTSGGRVASHEIMMATPAIRNLIREDKVAQMYSMIQTGSASGMQTMDQSI
KMLVAQGMVEAEEGRRVLDSAANRM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=88799 OCU77_RS02465 WP_048901013.1 500444..501481(+) (pilT) [Photobacterium swingsii strain CECT 7576]
ATGGATATCACCGAACTACTGGACTTTAGTGTAAAGCATAATGCCTCTGATCTGCATCTTTCTGCAGGGGTTCCACCAAT
TATTCGTGTTGACGGTGATGTACGTAAACTGAGTTTACCTGCTTTAGCGCATAGCGAGGTGCATCGTTTAGTTTTTGACA
TTATGAATGATGCACAGCGTCGTGAGTATGAAGAAAAGCTCGAAGTGGACTTTTCGTTCGAATTACCTGATGTAGGTCGC
TTTAGGGTGAATGCGTTTCACCAGTCTCGCGGCAGTGCCGCGGTTTTTCGTACCATCCCTATGAATGTGCCAACGCTAGA
GTCATTAAAAGTACCTGATATTTTTTACGATATCGCCCAGTTGCCGCGTGGCTTAGTGTTGGTAACGGGCGCAACGGGAT
CGGGTAAATCGACCACAATTGCGGCTTTGGTTGATTACATCAATGAAAACTATAACCGCCATATCCTGACGATTGAAGAT
CCGGTTGAATTTGTTCACCACAGCAAGCGTAGCTTGATTAACCAACGTGAAGTTCACCGCGATACCCACAGTTTTCAGGC
TGCGTTGCGTTCATCGTTGCGTGAAGATCCCGATGTGATTTTGGTGGGTGAGCTGCGAGATCAAGAAACCATTAGCTTGG
CGCTGACAGCGGCAGAAACGGGCCACTTAGTATTAGGAACACTGCATACCAGCTCAGCGGCGAAAACCATTGACCGTATT
ATCGATGTTTTCCCTGGTAATGATAAATCTATGGTGCGATCTATGTTGTCTGAGTCATTGCGGGCGGTGGTTTCACAAAG
TTTGTTGAAATGTACCAGTGGCGGTCGTGTTGCCTCCCATGAAATTATGATGGCGACTCCCGCGATCCGAAACTTGATCC
GCGAAGATAAAGTCGCACAAATGTATTCCATGATCCAAACCGGCTCAGCATCGGGGATGCAGACCATGGATCAAAGCATC
AAGATGCTGGTGGCACAAGGCATGGTAGAGGCTGAAGAAGGCCGACGTGTACTTGATAGCGCAGCAAACAGAATGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J8V4Q0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

78.378

96.522

0.757
  pilT Vibrio cholerae strain A1552

78.378

96.522

0.757
  pilT Acinetobacter baumannii D1279779

72.537

97.101

0.704
  pilT Acinetobacter baumannii strain A118

72.537

97.101

0.704
  pilT Acinetobacter nosocomialis M2

72.239

97.101

0.701
  pilT Acinetobacter baylyi ADP1

70.294

98.551

0.693
  pilT Pseudomonas aeruginosa PAK

68.955

97.101

0.67
  pilT Pseudomonas stutzeri DSM 10701

68.955

97.101

0.67
  pilT Legionella pneumophila strain Lp02

69.301

95.362

0.661
  pilT Legionella pneumophila strain ERS1305867

69.301

95.362

0.661
  pilT Neisseria meningitidis 8013

67.062

97.681

0.655
  pilT Neisseria gonorrhoeae MS11

66.766

97.681

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.733

98.841

0.501
  pilU Pseudomonas stutzeri DSM 10701

41.52

99.13

0.412
  pilU Vibrio cholerae strain A1552

41.228

99.13

0.409
  pilU Acinetobacter baylyi ADP1

37.101

100

0.371

Multiple sequence alignment