Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCU77_RS02470 Genome accession   NZ_AP024852
Coordinates   501491..502597 (+) Length   368 a.a.
NCBI ID   WP_048901014.1    Uniprot ID   A0A0J8XSU7
Organism   Photobacterium swingsii strain CECT 7576     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 496491..507597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCU77_RS02435 - 496542..497135 (-) 594 WP_048901008.1 XTP/dITP diphosphatase -
  OCU77_RS02440 - 497330..497758 (-) 429 WP_048901009.1 DUF4426 domain-containing protein -
  OCU77_RS02445 yggU 497925..498224 (-) 300 WP_048901010.1 DUF167 family protein YggU -
  OCU77_RS02450 - 498221..498778 (-) 558 WP_107303068.1 YggT family protein -
  OCU77_RS02455 proC 498788..499606 (-) 819 WP_048901011.1 pyrroline-5-carboxylate reductase -
  OCU77_RS02460 - 499710..500420 (-) 711 WP_048901012.1 YggS family pyridoxal phosphate-dependent enzyme -
  OCU77_RS02465 pilT 500444..501481 (+) 1038 WP_048901013.1 type IV pilus twitching motility protein PilT Machinery gene
  OCU77_RS02470 pilU 501491..502597 (+) 1107 WP_048901014.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCU77_RS02475 ruvX 502984..503415 (-) 432 WP_048901015.1 Holliday junction resolvase RuvX -
  OCU77_RS02480 - 503412..503975 (-) 564 WP_048901016.1 YqgE/AlgH family protein -
  OCU77_RS02485 gshB 504172..505119 (-) 948 WP_048901017.1 glutathione synthase -
  OCU77_RS02490 rsmE 505136..505867 (-) 732 WP_048901018.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCU77_RS02495 - 506016..506729 (-) 714 WP_107303067.1 endonuclease -
  OCU77_RS02500 - 506913..507395 (-) 483 WP_048901019.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41060.01 Da        Isoelectric Point: 5.7637

>NTDB_id=88800 OCU77_RS02470 WP_048901014.1 501491..502597(+) (pilU) [Photobacterium swingsii strain CECT 7576]
MTLQDILDQMVANKASDLYITVDSPCLLKIDGQLQAVGDKLDRAGVDALFADALDSQLTHEFKTTREANFALVRNELRFR
VSAFWQRELPGMVLRRIETQIPRLGDLSLPIDMERMALAKRGLVLVVGATGSGKSTSMAAMTGFRNSQRSGHILTVEDPI
EFVHQHDKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTPETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCVLAQQLIPDAHGVGRHAAFELLINTPRVADLIRRGELHEIKDTMAKGTDSGMMTFDQSLF
ELFSDGKITEQDALHHADSANDLRLMIKKGDKFQKGNSSLEGVTVSFD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=88800 OCU77_RS02470 WP_048901014.1 501491..502597(+) (pilU) [Photobacterium swingsii strain CECT 7576]
GTGACTTTACAAGACATTCTTGACCAGATGGTCGCGAACAAAGCCTCGGATCTTTATATCACGGTGGATTCGCCGTGTTT
GTTGAAAATAGACGGTCAACTACAAGCCGTCGGTGACAAGCTGGATCGTGCGGGTGTGGATGCACTGTTTGCTGATGCTT
TGGATTCTCAATTAACCCACGAATTTAAGACCACCCGCGAAGCCAATTTTGCCTTGGTACGTAATGAATTACGTTTTCGT
GTGAGTGCTTTTTGGCAGCGTGAGTTACCGGGTATGGTGTTGCGTCGTATCGAAACGCAAATTCCTCGCTTGGGTGATTT
ATCACTTCCCATTGATATGGAACGGATGGCACTGGCGAAGCGTGGTTTGGTGTTGGTTGTTGGGGCAACGGGATCGGGTA
AATCCACGTCGATGGCCGCCATGACTGGCTTTAGAAATAGTCAACGCAGTGGGCATATTTTAACGGTGGAAGATCCGATT
GAGTTTGTCCACCAGCATGATAAATGTATCGTGACCCAGCGTGAAGTTGGGCTTGATACCGAAAGTTATGAAGTGGCGCT
GAAGAACTCGTTGCGCCAAGCGCCTGACATGATTTTGATTGGTGAGATCCGTACGCCTGAAACCATGGAGTATGCGATGA
ATTTTGCCGAGACAGGCCACTTATGTATGGCAACCTTGCATGCTAATAATGCCAACCAAGCACTTGAGCGTATCTTGCAT
TTGGTGCCTAAAGAGCGTCGTGATCAGTTTTTGTTTGATCTGTCGTTGAACTTGAAATGTGTATTGGCACAGCAGTTGAT
CCCCGATGCGCATGGGGTCGGGCGACACGCCGCCTTTGAGCTTTTGATCAATACACCAAGGGTCGCAGATCTGATCCGTC
GTGGTGAATTACATGAAATAAAAGACACCATGGCGAAAGGGACTGACTCGGGAATGATGACCTTTGATCAATCCTTATTT
GAACTATTCAGTGATGGAAAAATTACTGAACAAGATGCGTTACACCATGCTGATTCAGCGAATGATTTGCGCTTAATGAT
CAAAAAAGGTGATAAATTCCAGAAAGGTAATAGTAGCTTAGAAGGAGTAACAGTGAGCTTTGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J8XSU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

69.189

100

0.696
  pilU Pseudomonas stutzeri DSM 10701

57.821

97.283

0.562
  pilU Acinetobacter baylyi ADP1

54.19

97.283

0.527
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.246

91.576

0.378
  pilT Legionella pneumophila strain Lp02

41.194

91.033

0.375
  pilT Legionella pneumophila strain ERS1305867

41.194

91.033

0.375

Multiple sequence alignment