Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RDV63_RS10595 Genome accession   NZ_CP135084
Coordinates   2172153..2173205 (-) Length   350 a.a.
NCBI ID   WP_313909472.1    Uniprot ID   -
Organism   Rheinheimera sp. MMS21-TC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2167153..2178205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV63_RS10575 (RDV63_10575) - 2168726..2169151 (-) 426 WP_313909469.1 HPP family protein -
  RDV63_RS10580 (RDV63_10580) - 2169272..2170135 (-) 864 WP_313909470.1 DMT family transporter -
  RDV63_RS10585 (RDV63_10585) - 2170321..2171013 (+) 693 WP_313910409.1 lytic transglycosylase domain-containing protein -
  RDV63_RS10590 (RDV63_10590) pilU 2171010..2172149 (-) 1140 WP_313909471.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RDV63_RS10595 (RDV63_10595) pilT 2172153..2173205 (-) 1053 WP_313909472.1 type IV pilus twitching motility protein PilT Machinery gene
  RDV63_RS10600 (RDV63_10600) - 2173236..2173922 (+) 687 WP_313909473.1 YggS family pyridoxal phosphate-dependent enzyme -
  RDV63_RS10605 (RDV63_10605) proC 2173931..2174746 (+) 816 WP_313909474.1 pyrroline-5-carboxylate reductase -
  RDV63_RS10610 (RDV63_10610) - 2174790..2175329 (+) 540 WP_313909475.1 YggT family protein -
  RDV63_RS10615 (RDV63_10615) yggU 2175329..2175622 (+) 294 WP_313909476.1 DUF167 family protein YggU -
  RDV63_RS10620 (RDV63_10620) - 2175679..2176104 (+) 426 WP_313909477.1 DUF4426 domain-containing protein -
  RDV63_RS10625 (RDV63_10625) rdgB 2176127..2176714 (+) 588 WP_313909478.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  RDV63_RS10630 (RDV63_10630) hemW 2176748..2177890 (+) 1143 WP_313909479.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 38803.54 Da        Isoelectric Point: 6.6935

>NTDB_id=884825 RDV63_RS10595 WP_313909472.1 2172153..2173205(-) (pilT) [Rheinheimera sp. MMS21-TC3]
MDITELLAFSVQHKASDLHLSAGVPPLIRVDGDVRRLNIPPLTHKEVHALIYEIMTDRQRKEYEENLESDFSFEVPNLAR
FRVNAFVQNRGAAAVFRTIPSEVLSLEDLGAPDIFKKIADNPRGLVLVTGPTGSGKSTTLAAMVDYINSMRHEHILTIED
PIEFVHSNKQCLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAEEKAMVRSMLSESLRAVISQTLLKKVGGGRIAAHEIMVGLPAIRNLIREDKIAQMYSVIQTGASHGMQTMDQCL
LNLVNRGLVSQQDAAGKAQDKNTFGNMGRM

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=884825 RDV63_RS10595 WP_313909472.1 2172153..2173205(-) (pilT) [Rheinheimera sp. MMS21-TC3]
ATGGATATTACTGAATTATTAGCCTTTAGTGTGCAACATAAAGCGTCAGATTTACACCTTTCTGCAGGGGTACCGCCTTT
AATACGTGTTGATGGTGATGTTAGAAGGCTTAACATTCCGCCATTAACTCATAAAGAGGTGCATGCCTTAATTTATGAGA
TTATGACAGATAGGCAGCGTAAAGAGTATGAAGAAAACCTAGAGTCTGACTTTTCATTTGAAGTACCAAACTTAGCTCGG
TTCAGGGTTAATGCTTTTGTACAAAATCGTGGTGCTGCTGCAGTATTTCGTACCATCCCAAGTGAAGTATTGTCACTTGA
AGACTTAGGTGCACCCGATATTTTCAAAAAAATTGCAGATAATCCTCGTGGTTTAGTACTTGTTACTGGACCTACCGGTT
CGGGTAAATCTACTACCTTAGCGGCTATGGTTGACTATATTAATAGTATGCGTCATGAGCATATATTAACCATTGAAGAC
CCAATAGAATTTGTTCATAGCAATAAGCAATGCTTAATTAACCAACGAGAAGTTCATCGTGATACCCACAGTTTTAGTAA
TGCTTTACGCTCTGCATTGCGTGAAGACCCGGATGTTATCTTAGTTGGTGAATTACGGGATTTAGAAACCATTCGTTTAG
CTATGACTGCAGCGGAAACAGGTCACTTAGTTTTTGGTACCTTGCATACTACCTCTGCACCTAAAACTATCGACCGTATT
ATCGATGTGTTTCCAGCAGAAGAAAAAGCTATGGTTCGCTCTATGTTATCGGAGTCTTTACGCGCAGTTATTTCGCAAAC
CTTATTGAAAAAAGTGGGTGGGGGCCGTATTGCCGCCCATGAAATTATGGTTGGTTTACCGGCAATCCGTAACTTGATTC
GTGAAGATAAAATTGCCCAAATGTATTCGGTAATTCAAACTGGTGCCTCGCATGGTATGCAAACTATGGATCAGTGTTTA
CTAAATCTAGTTAATCGTGGCTTAGTGTCACAACAAGATGCAGCAGGTAAAGCCCAAGATAAAAATACTTTTGGTAATAT
GGGTAGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.035

98.286

0.757
  pilT Acinetobacter baumannii strain A118

77.035

98.286

0.757
  pilT Acinetobacter nosocomialis M2

77.035

98.286

0.757
  pilT Pseudomonas aeruginosa PAK

76.453

98.286

0.751
  pilT Pseudomonas stutzeri DSM 10701

76.453

98.286

0.751
  pilT Acinetobacter baylyi ADP1

76.163

98.286

0.749
  pilT Legionella pneumophila strain Lp02

75

98.286

0.737
  pilT Legionella pneumophila strain ERS1305867

75

98.286

0.737
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.041

96.857

0.717
  pilT Vibrio cholerae strain A1552

74.041

96.857

0.717
  pilT Neisseria meningitidis 8013

67.826

98.571

0.669
  pilT Neisseria gonorrhoeae MS11

67.536

98.571

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.011

99.429

0.517
  pilU Vibrio cholerae strain A1552

42.566

98

0.417
  pilU Pseudomonas stutzeri DSM 10701

41.399

98

0.406
  pilU Acinetobacter baylyi ADP1

40.462

98.857

0.4