Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RDV63_RS10590 Genome accession   NZ_CP135084
Coordinates   2171010..2172149 (-) Length   379 a.a.
NCBI ID   WP_313909471.1    Uniprot ID   -
Organism   Rheinheimera sp. MMS21-TC3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2166010..2177149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDV63_RS10570 (RDV63_10570) - 2166499..2168655 (+) 2157 WP_313909468.1 S46 family peptidase -
  RDV63_RS10575 (RDV63_10575) - 2168726..2169151 (-) 426 WP_313909469.1 HPP family protein -
  RDV63_RS10580 (RDV63_10580) - 2169272..2170135 (-) 864 WP_313909470.1 DMT family transporter -
  RDV63_RS10585 (RDV63_10585) - 2170321..2171013 (+) 693 WP_313910409.1 lytic transglycosylase domain-containing protein -
  RDV63_RS10590 (RDV63_10590) pilU 2171010..2172149 (-) 1140 WP_313909471.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RDV63_RS10595 (RDV63_10595) pilT 2172153..2173205 (-) 1053 WP_313909472.1 type IV pilus twitching motility protein PilT Machinery gene
  RDV63_RS10600 (RDV63_10600) - 2173236..2173922 (+) 687 WP_313909473.1 YggS family pyridoxal phosphate-dependent enzyme -
  RDV63_RS10605 (RDV63_10605) proC 2173931..2174746 (+) 816 WP_313909474.1 pyrroline-5-carboxylate reductase -
  RDV63_RS10610 (RDV63_10610) - 2174790..2175329 (+) 540 WP_313909475.1 YggT family protein -
  RDV63_RS10615 (RDV63_10615) yggU 2175329..2175622 (+) 294 WP_313909476.1 DUF167 family protein YggU -
  RDV63_RS10620 (RDV63_10620) - 2175679..2176104 (+) 426 WP_313909477.1 DUF4426 domain-containing protein -
  RDV63_RS10625 (RDV63_10625) rdgB 2176127..2176714 (+) 588 WP_313909478.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41464.71 Da        Isoelectric Point: 5.6275

>NTDB_id=884824 RDV63_RS10590 WP_313909471.1 2171010..2172149(-) (pilU) [Rheinheimera sp. MMS21-TC3]
MSNLTDFLDKMVSMKASDMFVTAGMPVAAKINGEMTPIDETPLTDAGSLKLVLAAMNDKQQQEFMTTKECNFAIANEAGR
FRVSAFWQRDCAGMVVRRIITRIPEVDELGLPPIMKEIIMSKRGLVLFVGGTGTGKSTSLAALLGYRNRNTKGHILTIED
PIEFVHDHQKSLVTQREVGIDTESFGSALKSSLRQAPDVILIGEIRSQDTMEYALSFAETGHLCIATLHANNANQAIDRI
MHLVPKDMHQKLLFDLSLNLRAIVAQQLVPTADGQGRKAAIEVLLNSPLVADLIARGEIGEIKAVMTKSRELGMQTFDQA
LFDMYQSGIISYTDAIHHADSPNDLRLMIKLRSGDTKGAGFLSGVTIQGFEPDLDSDGN

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=884824 RDV63_RS10590 WP_313909471.1 2171010..2172149(-) (pilU) [Rheinheimera sp. MMS21-TC3]
ATGAGTAACTTGACTGATTTTCTTGATAAAATGGTGTCAATGAAGGCGTCTGATATGTTTGTTACCGCAGGGATGCCGGT
AGCGGCAAAAATAAATGGTGAAATGACGCCTATAGATGAAACGCCTCTTACCGATGCTGGATCATTAAAACTTGTGTTAG
CGGCAATGAATGACAAGCAACAACAAGAGTTTATGACTACTAAAGAGTGTAATTTTGCTATTGCCAATGAAGCCGGTCGT
TTTCGGGTGTCAGCATTTTGGCAGCGGGATTGCGCCGGCATGGTAGTACGGCGCATTATTACTCGTATACCCGAAGTAGA
TGAGCTTGGACTGCCACCTATAATGAAAGAAATTATCATGTCTAAACGAGGCTTGGTATTGTTTGTCGGGGGAACGGGTA
CCGGTAAATCGACTTCATTAGCAGCTTTGTTAGGCTATCGTAATCGTAATACTAAAGGTCATATTTTAACTATTGAAGAT
CCAATAGAATTTGTTCACGATCATCAAAAAAGCTTAGTGACTCAGCGTGAAGTTGGCATAGACACCGAATCTTTTGGTTC
AGCATTAAAAAGCTCATTGCGCCAAGCGCCAGATGTTATTTTAATTGGTGAGATCCGCAGCCAAGATACGATGGAATACG
CACTGTCTTTTGCTGAAACTGGCCATTTATGTATAGCGACGCTGCACGCCAATAATGCTAACCAAGCTATTGATAGGATC
ATGCACTTAGTACCAAAAGATATGCACCAAAAGTTGCTATTTGACTTATCACTCAACTTAAGGGCTATCGTTGCTCAGCA
GTTAGTGCCAACAGCAGATGGTCAAGGCCGTAAAGCGGCGATAGAGGTATTACTAAACTCTCCTTTAGTAGCAGATCTTA
TTGCTCGTGGCGAAATAGGTGAAATTAAAGCAGTAATGACCAAGTCACGTGAATTAGGTATGCAAACCTTTGACCAAGCA
TTATTTGATATGTATCAAAGTGGCATTATTAGCTACACAGATGCAATTCACCATGCAGATTCGCCAAACGATTTACGTCT
AATGATAAAGTTGCGTAGTGGTGATACTAAAGGGGCTGGCTTTTTATCCGGGGTAACTATACAAGGGTTTGAGCCAGATT
TAGATTCAGACGGTAATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.836

96.57

0.578
  pilU Acinetobacter baylyi ADP1

60.969

92.612

0.565
  pilU Vibrio cholerae strain A1552

57.692

96.042

0.554
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.442

90.765

0.385
  pilT Legionella pneumophila strain Lp02

42.598

87.335

0.372
  pilT Legionella pneumophila strain ERS1305867

42.598

87.335

0.372
  pilT Acinetobacter baylyi ADP1

40.058

90.237

0.361