Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ViNHUV68_RS01710 Genome accession   NZ_CP134975
Coordinates   342355..343392 (+) Length   345 a.a.
NCBI ID   WP_391089026.1    Uniprot ID   -
Organism   Vibrio sp. NH-UV-68     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337355..348392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ViNHUV68_RS01675 (ViNHUV68_03390) hemW 337564..338742 (-) 1179 WP_391089019.1 radical SAM family heme chaperone HemW -
  ViNHUV68_RS01680 (ViNHUV68_03400) - 338742..339338 (-) 597 WP_391089021.1 XTP/dITP diphosphatase -
  ViNHUV68_RS01685 (ViNHUV68_03410) - 339367..339798 (-) 432 WP_038173639.1 DUF4426 domain-containing protein -
  ViNHUV68_RS01690 (ViNHUV68_03420) yggU 339848..340138 (-) 291 WP_038173640.1 DUF167 family protein YggU -
  ViNHUV68_RS01695 (ViNHUV68_03430) - 340138..340695 (-) 558 WP_038173643.1 YggT family protein -
  ViNHUV68_RS01700 (ViNHUV68_03440) proC 340734..341552 (-) 819 WP_391089024.1 pyrroline-5-carboxylate reductase -
  ViNHUV68_RS01705 (ViNHUV68_03450) - 341613..342329 (-) 717 WP_038173645.1 YggS family pyridoxal phosphate-dependent enzyme -
  ViNHUV68_RS01710 (ViNHUV68_03460) pilT 342355..343392 (+) 1038 WP_391089026.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ViNHUV68_RS01715 (ViNHUV68_03470) pilU 343404..344510 (+) 1107 WP_391089028.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ViNHUV68_RS01720 (ViNHUV68_03480) ruvX 344599..345021 (-) 423 WP_038173649.1 Holliday junction resolvase RuvX -
  ViNHUV68_RS01725 (ViNHUV68_03490) - 345055..345618 (-) 564 WP_038173650.1 YqgE/AlgH family protein -
  ViNHUV68_RS01730 (ViNHUV68_03500) gshB 345667..346617 (-) 951 WP_038173651.1 glutathione synthase -
  ViNHUV68_RS01735 (ViNHUV68_03510) rsmE 346634..347365 (-) 732 WP_391089031.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ViNHUV68_RS01740 (ViNHUV68_03520) - 347602..348099 (-) 498 WP_391090235.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38268.82 Da        Isoelectric Point: 6.3173

>NTDB_id=884372 ViNHUV68_RS01710 WP_391089026.1 342355..343392(+) (pilT) [Vibrio sp. NH-UV-68]
MDITELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGVPPFSHSDVHRLVFDIMNDAQRREFEERLEADFSFELSDVGR
FRVNAFNQSRGCAAVFRTIPTEIPTLEQLATPQIFENIANMEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFSNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKEMVRSMLSESLKAVIAQKLIKRIGGGRIACHEIMMATPAIRNLIREDKVAQMVSVIQTGAAHGMQTMEQNA
KKLIAQGMVEQTEVAKKIDIESSMF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=884372 ViNHUV68_RS01710 WP_391089026.1 342355..343392(+) (pilT) [Vibrio sp. NH-UV-68]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAGCATAATGCGTCAGATCTACATCTTTCTGCTGGAGTATCTCCTAT
GGTGCGGATTGATGGGGAAGTTCGCAAGCTTGGTGTACCACCTTTTAGCCATTCAGACGTGCACCGTTTAGTGTTTGATA
TCATGAATGATGCTCAACGTCGTGAGTTTGAAGAACGTCTTGAGGCTGACTTTTCCTTTGAACTGTCAGATGTCGGACGT
TTTCGCGTCAATGCGTTCAACCAATCACGCGGCTGCGCGGCGGTGTTTCGTACTATCCCAACCGAAATTCCTACCTTAGA
GCAACTGGCAACGCCACAAATTTTTGAAAATATTGCCAATATGGAGAAAGGGTTAGTGCTGGTTACGGGGCCGACAGGAT
CGGGTAAATCGACGACGTTAGCGGCCATGGTTGATTACATCAATCGTAACCATAATAAGCACATCCTCACCATCGAAGAT
CCGATTGAATTTGTCCATAGCAACAATAAATGTTTGATTAACCAACGTGAAGTACACCGAGACACGCACAGTTTCAGCAA
TGCATTGCGCAGTGCACTGCGTGAAGATCCGGATGTGATCCTCGTGGGTGAGTTGCGCGATCAAGAAACGATCAGTCTGG
CGTTGACGGCGGCGGAAACGGGCCACTTAGTGTTTGGCACGTTGCATACCAGTTCAGCGGCGAAAACCATCGACCGAATT
ATTGATGTCTTCCCTGGTAGCGACAAAGAGATGGTGCGTTCAATGTTGTCTGAATCTCTTAAAGCGGTCATTGCGCAAAA
GCTGATTAAACGTATTGGTGGTGGCCGGATCGCCTGTCATGAGATCATGATGGCGACCCCTGCGATTCGTAACTTGATCC
GTGAAGATAAGGTCGCGCAAATGGTCTCCGTTATTCAAACGGGGGCAGCACATGGTATGCAAACGATGGAGCAAAATGCC
AAAAAGTTGATCGCTCAAGGTATGGTTGAACAAACCGAAGTGGCGAAAAAAATCGATATTGAATCGAGCATGTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

85.507

100

0.855
  pilT Vibrio cholerae strain A1552

85.507

100

0.855
  pilT Acinetobacter baumannii D1279779

71.733

95.362

0.684
  pilT Acinetobacter baumannii strain A118

71.733

95.362

0.684
  pilT Acinetobacter nosocomialis M2

71.429

95.362

0.681
  pilT Acinetobacter baylyi ADP1

70.213

95.362

0.67
  pilT Pseudomonas aeruginosa PAK

67.941

98.551

0.67
  pilT Pseudomonas stutzeri DSM 10701

67.656

97.681

0.661
  pilT Legionella pneumophila strain Lp02

65.507

100

0.655
  pilT Legionella pneumophila strain ERS1305867

65.507

100

0.655
  pilT Neisseria meningitidis 8013

64.201

97.971

0.629
  pilT Neisseria gonorrhoeae MS11

62.319

100

0.623
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.717

92.174

0.504
  pilU Vibrio cholerae strain A1552

41.765

98.551

0.412
  pilU Pseudomonas stutzeri DSM 10701

42.424

95.652

0.406
  pilU Acinetobacter baylyi ADP1

40.938

92.754

0.38