Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ViNHUV68_RS01715 Genome accession   NZ_CP134975
Coordinates   343404..344510 (+) Length   368 a.a.
NCBI ID   WP_391089028.1    Uniprot ID   -
Organism   Vibrio sp. NH-UV-68     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 338404..349510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ViNHUV68_RS01680 (ViNHUV68_03400) - 338742..339338 (-) 597 WP_391089021.1 XTP/dITP diphosphatase -
  ViNHUV68_RS01685 (ViNHUV68_03410) - 339367..339798 (-) 432 WP_038173639.1 DUF4426 domain-containing protein -
  ViNHUV68_RS01690 (ViNHUV68_03420) yggU 339848..340138 (-) 291 WP_038173640.1 DUF167 family protein YggU -
  ViNHUV68_RS01695 (ViNHUV68_03430) - 340138..340695 (-) 558 WP_038173643.1 YggT family protein -
  ViNHUV68_RS01700 (ViNHUV68_03440) proC 340734..341552 (-) 819 WP_391089024.1 pyrroline-5-carboxylate reductase -
  ViNHUV68_RS01705 (ViNHUV68_03450) - 341613..342329 (-) 717 WP_038173645.1 YggS family pyridoxal phosphate-dependent enzyme -
  ViNHUV68_RS01710 (ViNHUV68_03460) pilT 342355..343392 (+) 1038 WP_391089026.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ViNHUV68_RS01715 (ViNHUV68_03470) pilU 343404..344510 (+) 1107 WP_391089028.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ViNHUV68_RS01720 (ViNHUV68_03480) ruvX 344599..345021 (-) 423 WP_038173649.1 Holliday junction resolvase RuvX -
  ViNHUV68_RS01725 (ViNHUV68_03490) - 345055..345618 (-) 564 WP_038173650.1 YqgE/AlgH family protein -
  ViNHUV68_RS01730 (ViNHUV68_03500) gshB 345667..346617 (-) 951 WP_038173651.1 glutathione synthase -
  ViNHUV68_RS01735 (ViNHUV68_03510) rsmE 346634..347365 (-) 732 WP_391089031.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ViNHUV68_RS01740 (ViNHUV68_03520) - 347602..348099 (-) 498 WP_391090235.1 SprT family zinc-dependent metalloprotease -
  ViNHUV68_RS01745 (ViNHUV68_03530) - 348177..348974 (-) 798 WP_391089032.1 DUF2189 domain-containing protein -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41238.07 Da        Isoelectric Point: 5.9681

>NTDB_id=884373 ViNHUV68_RS01715 WP_391089028.1 343404..344510(+) (pilU) [Vibrio sp. NH-UV-68]
MDIDTCLQRMNDDKASDLYLTVGAPILYRIDGELRAEGEALNQTQLEALLNTMMDDQRQHDYVTTREANFAIVRDYGRYR
VSAFFQRELPGAVIRRIETTIPTIEQLKLPQVVKDLALAKRGLVLVVGATGSGKSTTMAAMTGYRNTSRSGHILTVEDPI
EFVHPHHQCIVTQREVGLDTESYEVALKNSLRQAPDMVVIGEIRSRETMQYAMMFAETGHLCLATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIGQQLIRDSQGQGRHGVFEVLLNTPRMADLIRRGDLHELKAAMAKSTQSGMQTFDQALY
QLVIEGKIDEQEAMHSADSANDLRMMIKTQRGTGFSSGSLADVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=884373 ViNHUV68_RS01715 WP_391089028.1 343404..344510(+) (pilU) [Vibrio sp. NH-UV-68]
ATGGATATTGATACCTGCCTGCAACGAATGAACGATGATAAAGCTTCGGATCTTTATTTAACAGTTGGAGCGCCTATTTT
GTATCGTATTGATGGTGAGTTGCGCGCTGAAGGTGAAGCTCTCAATCAAACACAGCTTGAGGCGCTGCTCAACACTATGA
TGGATGATCAGCGTCAACATGACTACGTGACCACTCGTGAAGCTAACTTTGCCATTGTGCGTGATTACGGCCGTTACCGA
GTGTCAGCCTTTTTTCAACGAGAGTTGCCCGGTGCGGTGATTCGTCGGATAGAAACCACCATTCCGACGATCGAGCAGCT
TAAATTGCCACAAGTGGTCAAGGATCTTGCGCTCGCCAAGCGAGGATTAGTGCTGGTGGTGGGCGCAACCGGGTCAGGTA
AGTCAACCACGATGGCTGCTATGACGGGCTACCGTAATACAAGTCGCAGCGGACACATATTGACGGTGGAAGATCCGATA
GAGTTTGTTCATCCGCATCACCAGTGCATTGTGACCCAGCGAGAAGTGGGGCTTGATACCGAAAGCTATGAGGTGGCGCT
AAAAAACTCATTGCGCCAAGCGCCAGATATGGTTGTGATTGGTGAGATCCGTAGTCGTGAAACCATGCAGTATGCGATGA
TGTTTGCCGAAACTGGCCATCTCTGTTTGGCGACGTTGCATGCCAACAATGCCAACCAGGCTTTAGAGCGTATTCTCCAT
TTGGTGCCCAAAGAGCAAAAAGAGCAGTTTTTGTTTGATCTGTCGATGAATTTACGTGGTGTGATTGGCCAACAATTGAT
TCGTGATAGCCAAGGGCAAGGTCGTCACGGTGTATTTGAAGTGTTACTGAATACGCCGCGGATGGCCGATTTAATTCGTC
GTGGTGATCTGCATGAGCTGAAAGCTGCGATGGCAAAATCGACCCAAAGTGGTATGCAGACTTTTGACCAAGCGCTGTAT
CAGCTAGTAATCGAGGGTAAGATTGACGAACAAGAGGCGATGCACAGCGCCGACTCGGCCAATGATTTGCGCATGATGAT
TAAGACTCAGCGCGGGACAGGTTTTTCGTCGGGGAGTTTAGCCGATGTGAAAATCGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

77.717

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

56.286

95.109

0.535
  pilU Acinetobacter baylyi ADP1

50.712

95.38

0.484
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.21

95.924

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.617

90.761

0.378
  pilT Vibrio cholerae strain A1552

41.617

90.761

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Acinetobacter baylyi ADP1

40.176

92.663

0.372
  pilT Pseudomonas aeruginosa PAK

40.413

92.12

0.372
  pilT Legionella pneumophila strain Lp02

40.118

92.12

0.37
  pilT Legionella pneumophila strain ERS1305867

40.118

92.12

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361