Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RKE25_RS18290 Genome accession   NZ_CP134867
Coordinates   4155786..4156823 (-) Length   345 a.a.
NCBI ID   WP_311839519.1    Uniprot ID   A0AA96IWV7
Organism   Dyella sp. BiH032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4150786..4161823
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE25_RS18280 (RKE25_18280) - 4153972..4154340 (+) 369 WP_311839517.1 BON domain-containing protein -
  RKE25_RS18285 (RKE25_18285) pilU 4154504..4155670 (-) 1167 WP_311839518.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKE25_RS18290 (RKE25_18290) pilT 4155786..4156823 (-) 1038 WP_311839519.1 type IV pilus twitching motility protein PilT Machinery gene
  RKE25_RS18295 (RKE25_18295) proC 4157181..4158005 (+) 825 WP_311839520.1 pyrroline-5-carboxylate reductase -
  RKE25_RS18300 (RKE25_18300) - 4158002..4158574 (+) 573 WP_311839521.1 YggT family protein -
  RKE25_RS18305 (RKE25_18305) - 4159112..4159927 (-) 816 WP_311839522.1 cobalamin-binding protein -
  RKE25_RS18310 (RKE25_18310) - 4160028..4160468 (-) 441 WP_311839523.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  RKE25_RS18315 (RKE25_18315) pdxH 4160479..4161066 (-) 588 WP_311839524.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38503.14 Da        Isoelectric Point: 6.8928

>NTDB_id=884215 RKE25_RS18290 WP_311839519.1 4155786..4156823(-) (pilT) [Dyella sp. BiH032]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFLEVDFSFEIPGLAR
FRVNAFNQNRGAGSVFRTIPSEVLTLEDLGAPRIFRELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHILTIED
PIEFVHTSQKCLVNQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRLAAHEIMVGIPAIRNLIREDKVAQMYSAIQTGQQYGMQTLDQNL
QDLVKRGLVTRQQAAAYARNKEVFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=884215 RKE25_RS18290 WP_311839519.1 4155786..4156823(-) (pilT) [Dyella sp. BiH032]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTGAAGAACAAGGCGTCGGACTTGCACCTGTCCGCCGGCTTGCCGCCGAT
GATCCGCGTGGACGGCGACGTCAGGCGCATCAATATTCCGGCGCTCGAGCACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAAGAATTCCTCGAAGTCGACTTCTCGTTCGAGATCCCCGGCCTGGCCCGT
TTCCGCGTGAACGCGTTCAACCAGAACCGCGGCGCCGGCAGCGTGTTCCGTACCATTCCCTCCGAAGTGCTCACCCTGGA
GGACCTGGGCGCCCCACGCATCTTCCGCGAGCTGATCGAGCAACCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTCGATCACATCAACAAGAACGAATACGGCCACATCCTCACCATCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGACCCCGACTACATCCTGGTGGGCGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGATTTCGCAGTC
GCTGCTCAAGAAGGTCGGCGGCGGACGCCTGGCGGCGCACGAGATCATGGTGGGCATCCCCGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCTGCCATCCAGACCGGCCAGCAATACGGCATGCAGACCTTGGACCAGAACTTG
CAAGACCTGGTCAAGCGCGGCCTGGTGACGCGCCAGCAGGCGGCGGCCTATGCCAGGAACAAGGAAGTGTTCAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Pseudomonas aeruginosa PAK

78.198

99.71

0.78
  pilT Acinetobacter baylyi ADP1

77.616

99.71

0.774
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.072

96.522

0.696
  pilT Vibrio cholerae strain A1552

72.072

96.522

0.696
  pilT Neisseria meningitidis 8013

65.889

99.42

0.655
  pilT Neisseria gonorrhoeae MS11

65.598

99.42

0.652
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.986

100

0.49
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391
  pilU Vibrio cholerae strain A1552

40

97.101

0.388