NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	RKE25_RS18285	Genome accession	NZ_CP134867
Coordinates	4154504..4155670 (-)	Length	388 a.a.
NCBI ID	WP_311839518.1	Uniprot ID	A0AA96IUI8
Organism	Dyella sp. BiH032
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4149504..4160670

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RKE25_RS18275 (RKE25_18275)	-	4150375..4153443 (-)	3069	WP_311839516.1	TonB-dependent receptor	-
RKE25_RS18280 (RKE25_18280)	-	4153972..4154340 (+)	369	WP_311839517.1	BON domain-containing protein	-
RKE25_RS18285 (RKE25_18285)	pilU	4154504..4155670 (-)	1167	WP_311839518.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RKE25_RS18290 (RKE25_18290)	pilT	4155786..4156823 (-)	1038	WP_311839519.1	type IV pilus twitching motility protein PilT	Machinery gene
RKE25_RS18295 (RKE25_18295)	proC	4157181..4158005 (+)	825	WP_311839520.1	pyrroline-5-carboxylate reductase	-
RKE25_RS18300 (RKE25_18300)	-	4158002..4158574 (+)	573	WP_311839521.1	YggT family protein	-
RKE25_RS18305 (RKE25_18305)	-	4159112..4159927 (-)	816	WP_311839522.1	cobalamin-binding protein	-
RKE25_RS18310 (RKE25_18310)	-	4160028..4160468 (-)	441	WP_311839523.1	secondary thiamine-phosphate synthase enzyme YjbQ	-

Sequence

Protein

Download Length: 388 a.a. Molecular weight: 43369.75 Da Isoelectric Point: 6.8177

                                >NTDB_id=884214 RKE25_RS18285 WP_311839518.1 4154504..4155670(-) (pilU) [Dyella sp. BiH032]

MSDFDFTSFLKLMVHKKASDLFITAGVAPSMKVQGRIVPITQSPLSAQQSRDMVLNVMTPAQREEFEKTHECQFAISAQG

VGRFRVSCFYQRNCVGMVLRRIEAKIPTVEELSLPPVIKQLAMTKRGIIIFVGGTGTGKSTSLAAMIGYRNANSTGHIIT

IEDPIEYVHKHEGCIITQRELGIDTDSWENALKNTLRQAPDVIMIGEVRTRETMEHAINFSETGHLCLCTLHANNANQAM

DRILHFFPEDRRQQLFMDLSLNLKGVVAQQLIPTPDGKARRVAVEILLGTPLVQDYIRQGEIHKLKEVMKESTNLGMKTF

DQSLVELYHAGEISYEDALRHADSSNEVRLRIKLAQGGDAHTLSQGLDGVELEETRDSNQFGGGMLYR

Nucleotide

Download Length: 1167 bp

                                >NTDB_id=884214 RKE25_RS18285 WP_311839518.1 4154504..4155670(-) (pilU) [Dyella sp. BiH032]

ATGAGCGACTTCGATTTCACCTCCTTCCTCAAGCTGATGGTCCACAAGAAGGCCTCGGACCTGTTCATCACGGCCGGCGT

CGCGCCGTCGATGAAGGTGCAGGGCCGCATCGTGCCCATCACGCAGAGCCCGCTGTCCGCGCAGCAGTCGCGCGACATGG

TGCTCAACGTCATGACGCCCGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAATGCCAGTTCGCCATCTCGGCGCAGGGC

GTGGGCCGCTTCCGTGTCTCGTGCTTCTACCAGCGCAACTGCGTGGGCATGGTGCTGCGCCGGATCGAGGCCAAGATCCC

GACGGTGGAGGAACTGAGCCTGCCGCCGGTGATCAAGCAGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG

GCACCGGCACCGGTAAGTCGACCTCGCTCGCGGCGATGATCGGCTACCGCAACGCCAACTCGACCGGGCACATCATCACC

ATCGAAGACCCGATCGAATACGTGCACAAGCACGAAGGCTGCATCATCACGCAGCGCGAGCTGGGCATCGATACCGACAG

CTGGGAGAACGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACGCGCGAGACGA

TGGAGCACGCCATCAACTTCTCCGAGACGGGCCACCTGTGCCTATGCACCCTGCACGCGAACAACGCCAACCAGGCGATG

GACCGCATCCTGCATTTCTTCCCGGAAGACCGCCGGCAGCAGCTGTTCATGGACCTGTCGCTCAACCTCAAGGGCGTGGT

GGCGCAGCAGCTCATTCCCACGCCGGACGGCAAGGCCCGCCGCGTGGCGGTGGAGATCCTGCTGGGCACGCCGCTGGTGC

AGGACTACATCCGCCAGGGCGAGATCCACAAGCTCAAGGAAGTGATGAAGGAATCCACCAACCTCGGCATGAAGACCTTC

GACCAGAGCCTGGTCGAGCTCTACCACGCCGGCGAGATCTCCTACGAGGACGCCCTGCGCCACGCCGACAGCTCCAACGA

AGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGACGCCCACACGCTTTCGCAGGGTCTGGACGGGGTGGAGCTGGAAG

AGACCCGCGATTCCAACCAGTTCGGCGGCGGGATGCTGTATCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Pseudomonas stutzeri DSM 10701	66.31	96.392	0.639
pilU	Acinetobacter baylyi ADP1	62.64	91.753	0.575
pilU	Vibrio cholerae strain A1552	52.528	91.753	0.482
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.054	95.876	0.384

Detailed information NTG0884214