Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RI132_RS12580 Genome accession   NZ_CP134783
Coordinates   2723871..2724977 (-) Length   368 a.a.
NCBI ID   WP_011079463.1    Uniprot ID   A0A3Q0L3V4
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718871..2729977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS12555 (RI132_12555) - 2719445..2720779 (-) 1335 WP_047108885.1 hypothetical protein -
  RI132_RS12560 (RI132_12560) rsmE 2721017..2721748 (+) 732 WP_047108887.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RI132_RS12565 (RI132_12565) gshB 2721763..2722716 (+) 954 WP_013571098.1 glutathione synthase -
  RI132_RS12570 (RI132_12570) - 2722771..2723334 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  RI132_RS12575 (RI132_12575) ruvX 2723356..2723778 (+) 423 WP_039554698.1 Holliday junction resolvase RuvX -
  RI132_RS12580 (RI132_12580) pilU 2723871..2724977 (-) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI132_RS12585 (RI132_12585) pilT 2724996..2726033 (-) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  RI132_RS12590 (RI132_12590) - 2726058..2726768 (+) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI132_RS12595 (RI132_12595) proC 2726803..2727621 (+) 819 WP_011079460.1 pyrroline-5-carboxylate reductase -
  RI132_RS12600 (RI132_12600) - 2727663..2728220 (+) 558 WP_013571095.1 YggT family protein -
  RI132_RS12605 (RI132_12605) yggU 2728220..2728510 (+) 291 WP_130190720.1 DUF167 family protein YggU -
  RI132_RS12610 (RI132_12610) - 2728553..2728984 (+) 432 WP_011079457.1 DUF4426 domain-containing protein -
  RI132_RS12615 (RI132_12615) - 2728995..2729597 (+) 603 WP_011079456.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41245.35 Da        Isoelectric Point: 6.7296

>NTDB_id=883739 RI132_RS12580 WP_011079463.1 2723871..2724977(-) (pilU) [Vibrio vulnificus strain GCU-01]
MDLNKILEGMITLKASDLYITVGAPVLFRVDGELRPQGEKLSQIEVAQLLDGAMDEERRADFRKSRESNFAIVRDSGRFR
VSAFYQRELPGAVIRRIETNIPTFEELKLPHVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKNGKGRHGVFEVLLNSPRVSDLIRRGDLHELKMTMAKSKEVGMQTFDQSLY
TLVVEGKISEEDAMHSADSANDLRLMLKTQRGDISSPGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=883739 RI132_RS12580 WP_011079463.1 2723871..2724977(-) (pilU) [Vibrio vulnificus strain GCU-01]
ATGGATTTAAACAAGATTTTAGAAGGGATGATCACGCTTAAAGCGTCGGATCTTTATATCACGGTTGGTGCCCCCGTGCT
GTTTCGTGTCGATGGCGAGCTGCGCCCGCAAGGCGAAAAATTATCGCAAATTGAAGTCGCTCAACTGCTGGATGGGGCGA
TGGACGAAGAGCGGCGCGCAGACTTTCGCAAAAGCCGCGAGTCAAACTTTGCCATTGTGCGAGATTCGGGGCGTTTTCGT
GTCAGTGCCTTCTACCAACGAGAGCTTCCTGGTGCGGTGATTCGCCGCATTGAAACCAACATTCCAACCTTTGAGGAACT
TAAGCTGCCTCATGTTTTGCAAGACTTAGCGATTGCCAAACGTGGATTGGTGCTGGTGGTGGGCGCGACGGGCTCTGGTA
AATCGACCACCATGGCTGCGATGACAGGATATCGAAATACCCACCGTAGTGGACACATTTTGACGGTGGAAGATCCGATT
GAATTTGTCCACGAACATAAACGCTGTATTGTCACCCAGCGTGAAGTCGGTTTGGATACGGAAAGCTATGAAGTCGCACT
AAAGAACTCGCTGCGTCAAGCGCCAGATATGATTCTAATTGGTGAGATTCGCAGTCGGGAAACCATGGAATACGCCATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCGACACTGCATGCCAATAACGCCAACCAAGCACTGGAGCGCATCTTACAC
TTGGTGCCTAAAGAACAAAAAGAGCAGTTTTTGTTTGACCTTTCCATGAACTTAAAAGGGGTGGTTGGTCAGCAGTTGAT
TCGTGATAAAAATGGTAAAGGTCGTCACGGTGTGTTCGAGGTGTTACTTAACAGCCCGCGCGTGTCGGATTTGATCCGTC
GTGGCGATTTGCATGAGCTGAAAATGACTATGGCGAAGTCGAAAGAAGTCGGTATGCAGACCTTTGACCAATCACTCTAT
ACATTAGTCGTGGAAGGCAAAATCAGCGAAGAAGACGCCATGCACAGTGCTGACTCTGCTAACGACTTGCGTTTGATGCT
GAAGACGCAGCGAGGTGATATTAGTTCCCCCGGCTCATTGGCGAACGTGAAGATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3V4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

59.429

95.109

0.565
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.753

88.043

0.394
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.413

92.12

0.372
  pilT Neisseria meningitidis 8013

40.06

90.217

0.361
  pilT Neisseria gonorrhoeae MS11

40.06

90.217

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.761

0.361
  pilT Vibrio cholerae strain A1552

39.82

90.761

0.361