Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RI132_RS12585 Genome accession   NZ_CP134783
Coordinates   2724996..2726033 (-) Length   345 a.a.
NCBI ID   WP_011079462.1    Uniprot ID   A0A3Q0L3W1
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719996..2731033
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS12560 (RI132_12560) rsmE 2721017..2721748 (+) 732 WP_047108887.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RI132_RS12565 (RI132_12565) gshB 2721763..2722716 (+) 954 WP_013571098.1 glutathione synthase -
  RI132_RS12570 (RI132_12570) - 2722771..2723334 (+) 564 WP_011079465.1 YqgE/AlgH family protein -
  RI132_RS12575 (RI132_12575) ruvX 2723356..2723778 (+) 423 WP_039554698.1 Holliday junction resolvase RuvX -
  RI132_RS12580 (RI132_12580) pilU 2723871..2724977 (-) 1107 WP_011079463.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI132_RS12585 (RI132_12585) pilT 2724996..2726033 (-) 1038 WP_011079462.1 type IV pilus twitching motility protein PilT Machinery gene
  RI132_RS12590 (RI132_12590) - 2726058..2726768 (+) 711 WP_011079461.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI132_RS12595 (RI132_12595) proC 2726803..2727621 (+) 819 WP_011079460.1 pyrroline-5-carboxylate reductase -
  RI132_RS12600 (RI132_12600) - 2727663..2728220 (+) 558 WP_013571095.1 YggT family protein -
  RI132_RS12605 (RI132_12605) yggU 2728220..2728510 (+) 291 WP_130190720.1 DUF167 family protein YggU -
  RI132_RS12610 (RI132_12610) - 2728553..2728984 (+) 432 WP_011079457.1 DUF4426 domain-containing protein -
  RI132_RS12615 (RI132_12615) - 2728995..2729597 (+) 603 WP_011079456.1 XTP/dITP diphosphatase -
  RI132_RS12620 (RI132_12620) hemW 2729607..2730776 (+) 1170 WP_072615923.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38515.16 Da        Isoelectric Point: 6.3696

>NTDB_id=883740 RI132_RS12585 WP_011079462.1 2724996..2726033(-) (pilT) [Vibrio vulnificus strain GCU-01]
MDITELLDFSVKHNASDLHLSAGVPPMVRIDGDVRKLGVPAFNHADVHRLVFEIMNDSQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCSAVFRTIPTNIPTLEELECPQIFEKIAKCEKGLVLVTGPTGSGKSTTLAAMVDYINRNYNKHILTIED
PIEFVHQNQKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
RQLIAQGKVAKEEVDAKIEIETIQF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=883740 RI132_RS12585 WP_011079462.1 2724996..2726033(-) (pilT) [Vibrio vulnificus strain GCU-01]
ATGGATATCACTGAGTTACTGGATTTTAGTGTAAAACATAACGCATCAGATCTACATCTTTCTGCGGGTGTGCCTCCTAT
GGTACGCATAGATGGCGATGTAAGAAAGCTCGGTGTGCCCGCATTTAATCATGCTGATGTGCATCGTTTGGTTTTTGAGA
TCATGAACGATTCACAGCGCAGTGAGTTTGAAGAGAAGTTGGAAGTCGATTTTTCCTTTGAATTGCCTAACGTAGGTCGT
TTTCGTGTTAACGCCTTCAACCAATCTCGCGGCTGCTCTGCGGTGTTTCGTACCATTCCAACCAATATCCCTACCTTGGA
AGAGCTTGAGTGCCCGCAAATTTTTGAAAAAATTGCGAAGTGTGAAAAGGGCTTGGTGTTGGTCACTGGGCCAACAGGTT
CGGGTAAATCGACCACGTTAGCGGCGATGGTGGATTACATTAACCGCAACTACAATAAACACATCCTGACCATTGAAGAT
CCGATTGAATTTGTTCACCAAAATCAGAAATGTTTGATTAACCAACGCGAAGTTCATCGCGACACCCACAGTTTTAAAAA
TGCCCTGCGCAGCGCATTGCGTGAGGACCCAGACGTTATCTTGGTGGGTGAGTTACGTGACCAAGAGACCATCAGCCTAG
CGCTTACCGCGGCAGAAACGGGCCACTTGGTGTTTGGTACGTTGCACACCAGTAGTGCGGCAAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGCAGTGACAAAGACATGGTGCGCTCAATGCTTTCTGAATCATTGCGCGCCGTTATCGCACAGAA
ATTGCTGAAACGCATTGGTGGTGGGCGAGTGGCGTGTCATGAGATCATGATGGCAACGCCCGCCATTCGTAACTTGATCC
GTGAAGATAAAGTGGCACAAATGTACTCCATTATCCAAACGGGGGCTGCGCACGGCATGCAGACGATGGAACAGAATGCG
CGTCAGTTGATTGCACAGGGCAAAGTGGCCAAAGAAGAAGTCGACGCCAAAATTGAGATTGAAACTATCCAGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3W1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.406

100

0.884
  pilT Vibrio cholerae strain A1552

88.406

100

0.884
  pilT Acinetobacter baumannii D1279779

72.892

96.232

0.701
  pilT Acinetobacter baumannii strain A118

72.892

96.232

0.701
  pilT Acinetobacter nosocomialis M2

72.59

96.232

0.699
  pilT Acinetobacter baylyi ADP1

72.477

94.783

0.687
  pilT Pseudomonas aeruginosa PAK

68.529

98.551

0.675
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Legionella pneumophila strain Lp02

68.58

95.942

0.658
  pilT Legionella pneumophila strain ERS1305867

68.58

95.942

0.658
  pilT Neisseria meningitidis 8013

66.066

96.522

0.638
  pilT Neisseria gonorrhoeae MS11

65.766

96.522

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.895

99.42

0.516
  pilU Pseudomonas stutzeri DSM 10701

42.515

96.812

0.412
  pilU Vibrio cholerae strain A1552

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40

92.754

0.371