Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   RI132_RS13245 Genome accession   NZ_CP134783
Coordinates   2859010..2860794 (-) Length   594 a.a.
NCBI ID   WP_011151276.1    Uniprot ID   Q7MH82
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2854010..2865794
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS13225 (RI132_13225) - 2854712..2855539 (-) 828 WP_039554836.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  RI132_RS13230 (RI132_13230) - 2855619..2857115 (-) 1497 WP_045623943.1 AAA family ATPase -
  RI132_RS13235 (RI132_13235) aroB 2857166..2858257 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  RI132_RS13240 (RI132_13240) aroK 2858290..2858808 (-) 519 WP_011079357.1 shikimate kinase AroK -
  RI132_RS13245 (RI132_13245) pilQ 2859010..2860794 (-) 1785 WP_011151276.1 type IV pilus secretin PilQ Machinery gene
  RI132_RS13250 (RI132_13250) pilP 2860808..2861320 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  RI132_RS13255 (RI132_13255) pilO 2861310..2861900 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  RI132_RS13260 (RI132_13260) pilN 2861893..2862483 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  RI132_RS13265 (RI132_13265) pilM 2862473..2863480 (-) 1008 WP_011079352.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64490.59 Da        Isoelectric Point: 4.5316

>NTDB_id=883743 RI132_RS13245 WP_011151276.1 2859010..2860794(-) (pilQ) [Vibrio vulnificus strain GCU-01]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGGEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=883743 RI132_RS13245 WP_011151276.1 2859010..2860794(-) (pilQ) [Vibrio vulnificus strain GCU-01]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCAGTGGCAGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCTAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGTAAGCTGATTTCTATCAACTTCCAAGATATTCCAGTACGTAACGTTTTGCAGCTGATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGGGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGTAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTTAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTTAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACCGTCGGCGGTTCAATCGAAAACAACCTGGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTGGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGCATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAATGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(186-234)

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7MH82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.729

99.327

0.732
  pilQ Vibrio cholerae strain A1552

73.729

99.327

0.732
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37