Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RI132_RS13255 Genome accession   NZ_CP134783
Coordinates   2861310..2861900 (-) Length   196 a.a.
NCBI ID   WP_011079354.1    Uniprot ID   A0A3Q0L3M6
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2856310..2866900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS13235 (RI132_13235) aroB 2857166..2858257 (-) 1092 WP_011079358.1 3-dehydroquinate synthase -
  RI132_RS13240 (RI132_13240) aroK 2858290..2858808 (-) 519 WP_011079357.1 shikimate kinase AroK -
  RI132_RS13245 (RI132_13245) pilQ 2859010..2860794 (-) 1785 WP_011151276.1 type IV pilus secretin PilQ Machinery gene
  RI132_RS13250 (RI132_13250) pilP 2860808..2861320 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  RI132_RS13255 (RI132_13255) pilO 2861310..2861900 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  RI132_RS13260 (RI132_13260) pilN 2861893..2862483 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  RI132_RS13265 (RI132_13265) pilM 2862473..2863480 (-) 1008 WP_011079352.1 type IV pilus assembly protein PilM Machinery gene
  RI132_RS13270 (RI132_13270) - 2863620..2866133 (+) 2514 WP_011079351.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22749.25 Da        Isoelectric Point: 5.0836

>NTDB_id=883745 RI132_RS13255 WP_011079354.1 2861310..2861900(-) (pilO) [Vibrio vulnificus strain GCU-01]
MASWQDLDIDEITDWPILPQAAVVVLLMLIIQGFGYWFYLKPETEVLQRMVTEEQTLKISARIKATKVAALPQLQSQLDE
LTQRYEFLLKQLPVQKELATMLASVNELGIQSKLTFTRIDWGEKQTKDFLYQLPLNIELTGDYHDIGEFSEAIARLPRII
SFTDVTWHRVSMESSTLHFRVKANTYQFKSEAVHEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=883745 RI132_RS13255 WP_011079354.1 2861310..2861900(-) (pilO) [Vibrio vulnificus strain GCU-01]
ATGGCTAGTTGGCAAGATCTCGATATTGATGAAATCACAGATTGGCCGATTTTACCGCAGGCGGCGGTTGTCGTGTTGCT
GATGTTGATCATTCAAGGTTTTGGTTATTGGTTCTATCTCAAGCCGGAAACGGAAGTGTTGCAAAGGATGGTCACAGAAG
AGCAAACGCTGAAAATCTCGGCTCGAATTAAGGCGACCAAAGTGGCTGCCTTGCCGCAACTTCAATCGCAACTTGATGAA
CTGACTCAGCGCTATGAATTCTTACTTAAGCAACTGCCCGTGCAGAAGGAGTTGGCGACGATGTTGGCGTCGGTCAATGA
ACTCGGCATTCAAAGTAAGCTCACGTTTACACGTATCGATTGGGGGGAAAAGCAAACCAAGGATTTCCTTTACCAACTGC
CTTTGAATATTGAGTTAACCGGGGATTATCACGATATCGGTGAATTTTCTGAAGCGATTGCCCGCTTGCCTCGCATTATC
AGTTTTACCGATGTGACTTGGCATCGAGTGAGTATGGAAAGCAGCACCTTGCACTTCCGAGTAAAAGCGAACACCTATCA
GTTCAAGTCGGAGGCTGTCCATGAGAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3M6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

73.016

96.429

0.704
  pilO Vibrio cholerae strain A1552

67.347

100

0.673