Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RI132_RS13265 Genome accession   NZ_CP134783
Coordinates   2862473..2863480 (-) Length   335 a.a.
NCBI ID   WP_011079352.1    Uniprot ID   -
Organism   Vibrio vulnificus strain GCU-01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2857473..2868480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI132_RS13240 (RI132_13240) aroK 2858290..2858808 (-) 519 WP_011079357.1 shikimate kinase AroK -
  RI132_RS13245 (RI132_13245) pilQ 2859010..2860794 (-) 1785 WP_011151276.1 type IV pilus secretin PilQ Machinery gene
  RI132_RS13250 (RI132_13250) pilP 2860808..2861320 (-) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  RI132_RS13255 (RI132_13255) pilO 2861310..2861900 (-) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  RI132_RS13260 (RI132_13260) pilN 2861893..2862483 (-) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  RI132_RS13265 (RI132_13265) pilM 2862473..2863480 (-) 1008 WP_011079352.1 type IV pilus assembly protein PilM Machinery gene
  RI132_RS13270 (RI132_13270) - 2863620..2866133 (+) 2514 WP_011079351.1 penicillin-binding protein 1A -
  RI132_RS13275 (RI132_13275) oxyR 2866204..2867103 (-) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  RI132_RS13280 (RI132_13280) - 2867377..2867649 (+) 273 WP_011079349.1 DUF3624 domain-containing protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37624.02 Da        Isoelectric Point: 6.3873

>NTDB_id=883747 RI132_RS13265 WP_011079352.1 2862473..2863480(-) (pilM) [Vibrio vulnificus strain GCU-01]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVALAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTSSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=883747 RI132_RS13265 WP_011079352.1 2862473..2863480(-) (pilM) [Vibrio vulnificus strain GCU-01]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGGCACTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCGATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCATTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCTAGCTATTGCTT
GTTGGATGTGGGTTATGACACCTCCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.731

100

0.537
  pilM Vibrio cholerae strain A1552

49.56

100

0.504