Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RKS75_RS03770 Genome accession   NZ_CP134599
Coordinates   788603..789640 (-) Length   345 a.a.
NCBI ID   WP_000355489.1    Uniprot ID   A0A009PTR1
Organism   Acinetobacter baumannii strain XH1015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 777181..821987 788603..789640 within 0


Gene organization within MGE regions


Location: 777181..821987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKS75_RS03710 (RKS75_03710) rep 777217..779256 (+) 2040 WP_000093035.1 DNA helicase Rep -
  RKS75_RS03715 (RKS75_03715) dut 779281..779733 (+) 453 WP_000868152.1 dUTP diphosphatase -
  RKS75_RS03720 (RKS75_03720) - 779933..781351 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  RKS75_RS03725 (RKS75_03725) argB 781366..782274 (+) 909 WP_001135419.1 acetylglutamate kinase -
  RKS75_RS03730 (RKS75_03730) - 782395..783177 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  RKS75_RS03735 (RKS75_03735) - 783286..784134 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  RKS75_RS03740 (RKS75_03740) - 784387..784842 (+) 456 WP_000782976.1 bacteriohemerythrin -
  RKS75_RS03745 (RKS75_03745) - 784995..786062 (+) 1068 WP_002019572.1 hypothetical protein -
  RKS75_RS03750 (RKS75_03750) - 786062..786385 (+) 324 WP_000442587.1 RnfH family protein -
  RKS75_RS03755 (RKS75_03755) - 786440..786838 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  RKS75_RS03760 (RKS75_03760) fur 786950..787387 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  RKS75_RS03765 (RKS75_03765) pilU 787457..788575 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKS75_RS03770 (RKS75_03770) pilT 788603..789640 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  RKS75_RS03775 (RKS75_03775) - 789767..790459 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  RKS75_RS03780 (RKS75_03780) - 790661..794257 (-) 3597 WP_000698785.1 AAA family ATPase -
  RKS75_RS03785 (RKS75_03785) - 794267..795535 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  RKS75_RS03790 (RKS75_03790) - 795699..796172 (+) 474 WP_000974358.1 OsmC family protein -
  RKS75_RS03795 (RKS75_03795) - 796240..796617 (-) 378 WP_001059438.1 VOC family protein -
  RKS75_RS03800 (RKS75_03800) - 796997..797449 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  RKS75_RS03805 (RKS75_03805) - 797539..798444 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  RKS75_RS03810 (RKS75_03810) - 798436..799551 (-) 1116 WP_000546701.1 FAD-binding oxidoreductase -
  RKS75_RS03815 (RKS75_03815) hemB 799577..800590 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  RKS75_RS03820 (RKS75_03820) - 800733..801230 (+) 498 WP_002019577.1 thioesterase family protein -
  RKS75_RS03825 (RKS75_03825) - 801500..802651 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  RKS75_RS03830 (RKS75_03830) - 802658..804181 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  RKS75_RS03835 (RKS75_03835) ggt 804484..806469 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  RKS75_RS03845 (RKS75_03845) - 807167..808324 (+) 1158 WP_000947475.1 site-specific integrase -
  RKS75_RS03850 (RKS75_03850) - 808321..809205 (+) 885 WP_001076477.1 endonuclease/exonuclease/phosphatase family protein -
  RKS75_RS03855 (RKS75_03855) - 809196..809651 (+) 456 WP_000549863.1 hypothetical protein -
  RKS75_RS03860 (RKS75_03860) - 809766..810758 (+) 993 WP_000527288.1 FRG domain-containing protein -
  RKS75_RS03865 (RKS75_03865) - 810765..810935 (-) 171 WP_000119264.1 hypothetical protein -
  RKS75_RS03870 (RKS75_03870) - 810936..811151 (-) 216 WP_001292077.1 hypothetical protein -
  RKS75_RS03875 (RKS75_03875) - 811148..811357 (-) 210 WP_000028957.1 hypothetical protein -
  RKS75_RS03880 (RKS75_03880) - 811354..811863 (-) 510 WP_000578497.1 phage protein -
  RKS75_RS03885 (RKS75_03885) - 811860..812396 (-) 537 WP_000609004.1 hypothetical protein -
  RKS75_RS03890 (RKS75_03890) - 812389..812568 (-) 180 WP_000464381.1 hypothetical protein -
  RKS75_RS03895 (RKS75_03895) - 812569..812859 (-) 291 WP_000986115.1 hypothetical protein -
  RKS75_RS03900 (RKS75_03900) - 813000..814028 (-) 1029 WP_000805204.1 ead/Ea22-like family protein -
  RKS75_RS03905 (RKS75_03905) - 814041..814721 (-) 681 WP_000643997.1 PolC-type DNA polymerase III -
  RKS75_RS03910 (RKS75_03910) - 814892..815263 (-) 372 WP_001260065.1 hypothetical protein -
  RKS75_RS03915 (RKS75_03915) - 815260..815589 (-) 330 WP_001072368.1 hypothetical protein -
  RKS75_RS03920 (RKS75_03920) - 815648..815908 (-) 261 WP_000350171.1 hypothetical protein -
  RKS75_RS03925 (RKS75_03925) - 815994..816797 (-) 804 WP_001290754.1 DUF2303 family protein -
  RKS75_RS03930 (RKS75_03930) - 816838..817170 (-) 333 WP_000105905.1 hypothetical protein -
  RKS75_RS03935 (RKS75_03935) - 817382..817669 (-) 288 WP_000440996.1 hypothetical protein -
  RKS75_RS03940 (RKS75_03940) - 817680..818378 (-) 699 WP_000660939.1 XRE family transcriptional regulator -
  RKS75_RS03945 (RKS75_03945) - 818515..818748 (+) 234 WP_000335917.1 helix-turn-helix domain-containing protein -
  RKS75_RS03950 (RKS75_03950) - 818781..819155 (+) 375 WP_000818520.1 hypothetical protein -
  RKS75_RS03955 (RKS75_03955) - 819190..819390 (+) 201 WP_000290738.1 hypothetical protein -
  RKS75_RS03960 (RKS75_03960) - 819387..819572 (+) 186 WP_000200038.1 hypothetical protein -
  RKS75_RS03965 (RKS75_03965) - 819569..820024 (+) 456 WP_000575723.1 hypothetical protein -
  RKS75_RS03970 (RKS75_03970) - 820021..820662 (+) 642 WP_000218438.1 hypothetical protein -
  RKS75_RS03975 (RKS75_03975) - 820662..821222 (+) 561 WP_000410652.1 hypothetical protein -
  RKS75_RS03980 (RKS75_03980) - 821228..821614 (+) 387 WP_002042962.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38312.92 Da        Isoelectric Point: 6.7271

>NTDB_id=882210 RKS75_RS03770 WP_000355489.1 788603..789640(-) (pilT) [Acinetobacter baumannii strain XH1015]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMLDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KGLVARGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=882210 RKS75_RS03770 WP_000355489.1 788603..789640(-) (pilT) [Acinetobacter baumannii strain XH1015]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATCTTTCTGCTGGCATGCCACCAAT
GATTCGTGTTGACGGTGAAGTTCGTCGTATTAACTTACCAGCTCTAGAACATAAAGATGTCCACCGTCTTGTTTACGACA
TCATGAATGATAAACAGCGCCGTGACTATGAAGAAAAACTCGAAACTGACTTTTCTTTTGAAGTACCTAATGTTGCGCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCCGGTGCAGTATTTCGTACCATTCCATCTAAAGTCCTCACCATGGA
AGATCTAGGTCTAGGACAAATCTTTAAAGATATTTGCGACTATCCTCGTGGCATTGTACTGGTGACAGGACCAACAGGTT
CAGGTAAATCAACTACGCTTGCAGCGATGCTCGATTATATTAACGAAAACCGCTATGACCATATTTTAACGGTGGAAGAT
CCAATCGAATTTGTACACCAGTCTAAGAAATGTCTCATTAACCAGCGTGAAGTGCATCGTGACACACACGGCTTTAATGA
AGCACTTCGCTCAGCACTGCGTGAAGACCCTGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAAACTATTCGCTTGG
CATTAACCGCTGCCGAAACAGGTCACTTGGTGTTTGGTACACTACACACCACGTCGGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCTGCCGAAGAAAAAGACATGGTTCGTGCCATGCTGTCAGAATCTTTACAAGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGTGGTGGCCGTGTCGCAGCACATGAGATCATGATTGGTATTCCGGCTATTCGTAACTTAATTC
GTGAAAACAAAGTCGCACAAATGTACTCAGCCATTCAAACTGGTGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAAGGCTTAGTCGCTCGCGGTGTGATTAGCCCACAAACCGCACGTACTGCGGCAAAACAGCCTGAATCCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A009PTR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

100

100

1
  pilT Acinetobacter baumannii D1279779

100

100

1
  pilT Acinetobacter nosocomialis M2

99.71

100

0.997
  pilT Acinetobacter baylyi ADP1

88.953

99.71

0.887
  pilT Pseudomonas aeruginosa PAK

84.302

99.71

0.841
  pilT Pseudomonas stutzeri DSM 10701

84.012

99.71

0.838
  pilT Legionella pneumophila strain Lp02

75.581

99.71

0.754
  pilT Legionella pneumophila strain ERS1305867

75.581

99.71

0.754
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.726

97.391

0.699
  pilT Vibrio cholerae strain A1552

71.726

97.391

0.699
  pilT Neisseria meningitidis 8013

68.116

100

0.681
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.678
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.597

97.101

0.394
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388