Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RKS75_RS03765 Genome accession   NZ_CP134599
Coordinates   787457..788575 (-) Length   372 a.a.
NCBI ID   WP_000347039.1    Uniprot ID   A0A9P2XLL8
Organism   Acinetobacter baumannii strain XH1015     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 777181..821987 787457..788575 within 0


Gene organization within MGE regions


Location: 777181..821987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKS75_RS03710 (RKS75_03710) rep 777217..779256 (+) 2040 WP_000093035.1 DNA helicase Rep -
  RKS75_RS03715 (RKS75_03715) dut 779281..779733 (+) 453 WP_000868152.1 dUTP diphosphatase -
  RKS75_RS03720 (RKS75_03720) - 779933..781351 (+) 1419 WP_001102845.1 phosphomannomutase/phosphoglucomutase -
  RKS75_RS03725 (RKS75_03725) argB 781366..782274 (+) 909 WP_001135419.1 acetylglutamate kinase -
  RKS75_RS03730 (RKS75_03730) - 782395..783177 (+) 783 WP_000890283.1 GNAT family N-acetyltransferase -
  RKS75_RS03735 (RKS75_03735) - 783286..784134 (+) 849 WP_000336548.1 class II glutamine amidotransferase -
  RKS75_RS03740 (RKS75_03740) - 784387..784842 (+) 456 WP_000782976.1 bacteriohemerythrin -
  RKS75_RS03745 (RKS75_03745) - 784995..786062 (+) 1068 WP_002019572.1 hypothetical protein -
  RKS75_RS03750 (RKS75_03750) - 786062..786385 (+) 324 WP_000442587.1 RnfH family protein -
  RKS75_RS03755 (RKS75_03755) - 786440..786838 (-) 399 WP_001170994.1 outer membrane protein assembly factor BamE -
  RKS75_RS03760 (RKS75_03760) fur 786950..787387 (+) 438 WP_001122847.1 ferric iron uptake transcriptional regulator -
  RKS75_RS03765 (RKS75_03765) pilU 787457..788575 (-) 1119 WP_000347039.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKS75_RS03770 (RKS75_03770) pilT 788603..789640 (-) 1038 WP_000355489.1 type IV pilus twitching motility protein PilT Machinery gene
  RKS75_RS03775 (RKS75_03775) - 789767..790459 (+) 693 WP_001108512.1 YggS family pyridoxal phosphate-dependent enzyme -
  RKS75_RS03780 (RKS75_03780) - 790661..794257 (-) 3597 WP_000698785.1 AAA family ATPase -
  RKS75_RS03785 (RKS75_03785) - 794267..795535 (-) 1269 WP_000116701.1 exonuclease SbcCD subunit D -
  RKS75_RS03790 (RKS75_03790) - 795699..796172 (+) 474 WP_000974358.1 OsmC family protein -
  RKS75_RS03795 (RKS75_03795) - 796240..796617 (-) 378 WP_001059438.1 VOC family protein -
  RKS75_RS03800 (RKS75_03800) - 796997..797449 (+) 453 WP_000164153.1 ABZJ_00895 family protein -
  RKS75_RS03805 (RKS75_03805) - 797539..798444 (+) 906 WP_001039356.1 TIGR01777 family oxidoreductase -
  RKS75_RS03810 (RKS75_03810) - 798436..799551 (-) 1116 WP_000546701.1 FAD-binding oxidoreductase -
  RKS75_RS03815 (RKS75_03815) hemB 799577..800590 (-) 1014 WP_000222569.1 porphobilinogen synthase -
  RKS75_RS03820 (RKS75_03820) - 800733..801230 (+) 498 WP_002019577.1 thioesterase family protein -
  RKS75_RS03825 (RKS75_03825) - 801500..802651 (+) 1152 WP_000128703.1 EmrA/EmrK family multidrug efflux transporter periplasmic adaptor subunit -
  RKS75_RS03830 (RKS75_03830) - 802658..804181 (+) 1524 WP_000857095.1 DHA2 family efflux MFS transporter permease subunit -
  RKS75_RS03835 (RKS75_03835) ggt 804484..806469 (+) 1986 WP_001042923.1 gamma-glutamyltransferase -
  RKS75_RS03845 (RKS75_03845) - 807167..808324 (+) 1158 WP_000947475.1 site-specific integrase -
  RKS75_RS03850 (RKS75_03850) - 808321..809205 (+) 885 WP_001076477.1 endonuclease/exonuclease/phosphatase family protein -
  RKS75_RS03855 (RKS75_03855) - 809196..809651 (+) 456 WP_000549863.1 hypothetical protein -
  RKS75_RS03860 (RKS75_03860) - 809766..810758 (+) 993 WP_000527288.1 FRG domain-containing protein -
  RKS75_RS03865 (RKS75_03865) - 810765..810935 (-) 171 WP_000119264.1 hypothetical protein -
  RKS75_RS03870 (RKS75_03870) - 810936..811151 (-) 216 WP_001292077.1 hypothetical protein -
  RKS75_RS03875 (RKS75_03875) - 811148..811357 (-) 210 WP_000028957.1 hypothetical protein -
  RKS75_RS03880 (RKS75_03880) - 811354..811863 (-) 510 WP_000578497.1 phage protein -
  RKS75_RS03885 (RKS75_03885) - 811860..812396 (-) 537 WP_000609004.1 hypothetical protein -
  RKS75_RS03890 (RKS75_03890) - 812389..812568 (-) 180 WP_000464381.1 hypothetical protein -
  RKS75_RS03895 (RKS75_03895) - 812569..812859 (-) 291 WP_000986115.1 hypothetical protein -
  RKS75_RS03900 (RKS75_03900) - 813000..814028 (-) 1029 WP_000805204.1 ead/Ea22-like family protein -
  RKS75_RS03905 (RKS75_03905) - 814041..814721 (-) 681 WP_000643997.1 PolC-type DNA polymerase III -
  RKS75_RS03910 (RKS75_03910) - 814892..815263 (-) 372 WP_001260065.1 hypothetical protein -
  RKS75_RS03915 (RKS75_03915) - 815260..815589 (-) 330 WP_001072368.1 hypothetical protein -
  RKS75_RS03920 (RKS75_03920) - 815648..815908 (-) 261 WP_000350171.1 hypothetical protein -
  RKS75_RS03925 (RKS75_03925) - 815994..816797 (-) 804 WP_001290754.1 DUF2303 family protein -
  RKS75_RS03930 (RKS75_03930) - 816838..817170 (-) 333 WP_000105905.1 hypothetical protein -
  RKS75_RS03935 (RKS75_03935) - 817382..817669 (-) 288 WP_000440996.1 hypothetical protein -
  RKS75_RS03940 (RKS75_03940) - 817680..818378 (-) 699 WP_000660939.1 XRE family transcriptional regulator -
  RKS75_RS03945 (RKS75_03945) - 818515..818748 (+) 234 WP_000335917.1 helix-turn-helix domain-containing protein -
  RKS75_RS03950 (RKS75_03950) - 818781..819155 (+) 375 WP_000818520.1 hypothetical protein -
  RKS75_RS03955 (RKS75_03955) - 819190..819390 (+) 201 WP_000290738.1 hypothetical protein -
  RKS75_RS03960 (RKS75_03960) - 819387..819572 (+) 186 WP_000200038.1 hypothetical protein -
  RKS75_RS03965 (RKS75_03965) - 819569..820024 (+) 456 WP_000575723.1 hypothetical protein -
  RKS75_RS03970 (RKS75_03970) - 820021..820662 (+) 642 WP_000218438.1 hypothetical protein -
  RKS75_RS03975 (RKS75_03975) - 820662..821222 (+) 561 WP_000410652.1 hypothetical protein -
  RKS75_RS03980 (RKS75_03980) - 821228..821614 (+) 387 WP_002042962.1 hypothetical protein -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41933.13 Da        Isoelectric Point: 6.6944

>NTDB_id=882209 RKS75_RS03765 WP_000347039.1 787457..788575(-) (pilU) [Acinetobacter baumannii strain XH1015]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=882209 RKS75_RS03765 WP_000347039.1 787457..788575(-) (pilU) [Acinetobacter baumannii strain XH1015]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(31-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.926

100

0.772
  pilU Pseudomonas stutzeri DSM 10701

69.565

98.925

0.688
  pilU Vibrio cholerae strain A1552

53.243

99.462

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.486

93.011

0.395