Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHP75_RS19170 Genome accession   NZ_CP134499
Coordinates   4056251..4057285 (-) Length   344 a.a.
NCBI ID   WP_090250115.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20056     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4051251..4062285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP75_RS19150 (RHP75_19150) - 4052391..4052789 (-) 399 WP_311089592.1 TM2 domain-containing protein -
  RHP75_RS19155 (RHP75_19155) - 4052944..4054155 (+) 1212 WP_311089593.1 putative DNA modification/repair radical SAM protein -
  RHP75_RS19160 (RHP75_19160) - 4054155..4055033 (+) 879 WP_311089594.1 TIGR03915 family putative DNA repair protein -
  RHP75_RS19165 (RHP75_19165) pilU 4054999..4056144 (-) 1146 WP_311089595.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHP75_RS19170 (RHP75_19170) pilT 4056251..4057285 (-) 1035 WP_090250115.1 type IV pilus twitching motility protein PilT Machinery gene
  RHP75_RS19175 (RHP75_19175) - 4057349..4058035 (+) 687 WP_311089596.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHP75_RS19180 (RHP75_19180) proC 4058072..4058890 (+) 819 WP_311089597.1 pyrroline-5-carboxylate reductase -
  RHP75_RS19185 (RHP75_19185) - 4058900..4059490 (+) 591 WP_311089598.1 YggT family protein -
  RHP75_RS19190 (RHP75_19190) - 4059503..4059799 (+) 297 WP_311089599.1 DUF167 domain-containing protein -
  RHP75_RS19195 (RHP75_19195) - 4060035..4062017 (+) 1983 WP_311089601.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37959.65 Da        Isoelectric Point: 6.8361

>NTDB_id=880586 RHP75_RS19170 WP_090250115.1 4056251..4057285(-) (pilT) [Pseudomonas sp. SG20056]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLSMEDLGMGEIFRKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLVTKGIVSREAAREKAKTPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=880586 RHP75_RS19170 WP_090250115.1 4056251..4057285(-) (pilT) [Pseudomonas sp. SG20056]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCATCTCTCCGCTGGCCTGCCTCCGAT
GATTCGGGTCGACGGTGATGTTCGCCGGATCAATTTGCCTGCGCTGGACCATAAACAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTCCTCGAGACCGATTTCTCCTTCGAAGTGCCGGGCGTGGCGCGT
TTCCGGGTTAACGCCTTCAACCAGAACCGCGGTTCTGGCGCGGTATTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTTGGCATGGGCGAAATCTTCCGCAAGATTTCCGATGTGCCGCGCGGCCTGGTGCTGGTGACCGGGCCAACCGGTT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTTGACTATATAAACAGCAACAAGTACCACCACATCCTGACGGTCGAAGAC
CCGATCGAATTTGTTCACGAATCGAAGAAGTGCCTGATCAACCAGCGTGAGGTGCACCGCGACACCCTCGGCTTCTCCGA
AGCCCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCAGCAGAAACCGGCCACCTGGTGTTTGGCACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGCGTG
GTCGACGTATTCCCGGCCGAAGAGAAATCCATGGTGCGCTCCATGCTCTCCGAATCGCTGCAATCGGTTATCTCGCAGAC
TCTGCTGAAGAAGATCGGCGGCGGTCGTGTGGCGGCCCACGAAATCATGATCGGCACCCCGGCCATCCGTAACCTGATTC
GTGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACCGGTGGCTCGCTGGGCATGCAGACCCTGGATTCCTGCCTG
AAAACTCTGGTGACCAAGGGCATCGTCAGCCGCGAAGCGGCGCGCGAGAAAGCCAAAACACCAGAAAACTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Pseudomonas aeruginosa PAK

93.314

100

0.933
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter baylyi ADP1

82.849

100

0.828
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.546

97.965

0.672
  pilT Vibrio cholerae strain A1552

68.546

97.965

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384