Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RHP75_RS19165 Genome accession   NZ_CP134499
Coordinates   4054999..4056144 (-) Length   381 a.a.
NCBI ID   WP_311089595.1    Uniprot ID   -
Organism   Pseudomonas sp. SG20056     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4049999..4061144
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHP75_RS19145 (RHP75_19145) - 4050991..4052262 (+) 1272 WP_311089591.1 dihydroorotase -
  RHP75_RS19150 (RHP75_19150) - 4052391..4052789 (-) 399 WP_311089592.1 TM2 domain-containing protein -
  RHP75_RS19155 (RHP75_19155) - 4052944..4054155 (+) 1212 WP_311089593.1 putative DNA modification/repair radical SAM protein -
  RHP75_RS19160 (RHP75_19160) - 4054155..4055033 (+) 879 WP_311089594.1 TIGR03915 family putative DNA repair protein -
  RHP75_RS19165 (RHP75_19165) pilU 4054999..4056144 (-) 1146 WP_311089595.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHP75_RS19170 (RHP75_19170) pilT 4056251..4057285 (-) 1035 WP_090250115.1 type IV pilus twitching motility protein PilT Machinery gene
  RHP75_RS19175 (RHP75_19175) - 4057349..4058035 (+) 687 WP_311089596.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHP75_RS19180 (RHP75_19180) proC 4058072..4058890 (+) 819 WP_311089597.1 pyrroline-5-carboxylate reductase -
  RHP75_RS19185 (RHP75_19185) - 4058900..4059490 (+) 591 WP_311089598.1 YggT family protein -
  RHP75_RS19190 (RHP75_19190) - 4059503..4059799 (+) 297 WP_311089599.1 DUF167 domain-containing protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42546.76 Da        Isoelectric Point: 6.6648

>NTDB_id=880585 RHP75_RS19165 WP_311089595.1 4054999..4056144(-) (pilU) [Pseudomonas sp. SG20056]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHSVMNEQQRRDFAENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPSDRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSQGEITYEDALLYADSANDLRLMIKLGSETDGDHLSSMAQGLSLEVSEEDPGRRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=880585 RHP75_RS19165 WP_311089595.1 4054999..4056144(-) (pilU) [Pseudomonas sp. SG20056]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTTATTACCGCTGGCGTACCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGATCACCAAAACGCCCATGTCGCCTGAGCAGACCCGTGAAACCGTGCATTCGG
TGATGAACGAGCAGCAGCGCCGCGACTTTGCCGAGAACCACGAATGCAACTTCGCCATCAGCGCCCGTGGTGTTGGCCGT
TTCCGCGTCAGTGCCTTCTACCAGCGCAACCTGGCCGGTATGGTGCTGCGTCGTATCGAGACCAATATTCCGACGCTGGA
CGAGCTGAAACTGCCGGAAATTCTCAAGAAACTGGCGCTGACCAAGCGCGGTCTGGTGTTGTTTGTCGGTGCGACTGGTA
CCGGTAAGTCGACTTCCCTGGCGGCGATGATCGGCTACCGCAACAAGAACAGCAGCGGCCACATCATTTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGAGCTGTATCGTTACCCAGCGTGAAGTGGGCATCGATACCGAGTCTTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATTGGTGAGGTGCGTACCCGCGAGACCATGGATCATG
CCGTGGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCCACACTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCTCTGACCGGCAGAACCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
ACTGATCCCGACCCCGGATGGCAAAGGCCGCCGTGCGGTGATTGAGGTATTGATCAACACCCCGCTGGCCGCCGATCTGA
TCCGCAAGGGTGAGGTGCACGAACTCAAGGCGCTGATGAAACGCTCCACCGAGCAGGGCATGCAGACCTTCGATCAGGCG
CTGTACAACCTCTACAGCCAGGGTGAGATCACCTACGAAGATGCGCTGCTGTACGCCGATTCGGCCAACGATCTGCGCCT
GATGATCAAGCTCGGCTCGGAAACCGATGGCGATCACCTGAGCAGCATGGCCCAGGGCTTGTCGCTGGAAGTCAGCGAAG
AAGATCCGGGTCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.339

100

0.913
  pilU Acinetobacter baylyi ADP1

64.146

93.701

0.601
  pilU Vibrio cholerae strain A1552

59.143

91.864

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

88.189

0.386
  pilT Neisseria gonorrhoeae MS11

41.493

87.927

0.365
  pilT Neisseria meningitidis 8013

41.493

87.927

0.365
  pilT Pseudomonas aeruginosa PAK

41.194

87.927

0.362