Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RJD39_RS14460 Genome accession   NZ_CP134277
Coordinates   3067378..3068409 (+) Length   343 a.a.
NCBI ID   WP_406732564.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain 21FBVib0096     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3062378..3073409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD39_RS14425 (RJD39_14405) hemW 3062630..3063805 (-) 1176 WP_406732557.1 radical SAM family heme chaperone HemW -
  RJD39_RS14430 (RJD39_14410) - 3063809..3064405 (-) 597 WP_009385807.1 XTP/dITP diphosphatase -
  RJD39_RS14435 (RJD39_14415) - 3064418..3064849 (-) 432 WP_005595631.1 DUF4426 domain-containing protein -
  RJD39_RS14440 (RJD39_14420) yggU 3064880..3065170 (-) 291 WP_406732560.1 DUF167 family protein YggU -
  RJD39_RS14445 (RJD39_14425) - 3065170..3065727 (-) 558 WP_406732562.1 YggT family protein -
  RJD39_RS14450 (RJD39_14430) proC 3065769..3066587 (-) 819 WP_069445969.1 pyrroline-5-carboxylate reductase -
  RJD39_RS14455 (RJD39_14435) - 3066637..3067353 (-) 717 WP_009385811.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJD39_RS14460 (RJD39_14440) pilT 3067378..3068409 (+) 1032 WP_406732564.1 type IV pilus twitching motility protein PilT Machinery gene
  RJD39_RS14465 (RJD39_14445) pilU 3068424..3069524 (+) 1101 WP_406732566.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJD39_RS14470 (RJD39_14450) lysC 3069588..3070940 (-) 1353 WP_069445972.1 lysine-sensitive aspartokinase 3 -
  RJD39_RS14475 (RJD39_14455) - 3071405..3072526 (+) 1122 WP_406732567.1 pyridoxal-phosphate-dependent aminotransferase family protein -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 37796.29 Da        Isoelectric Point: 5.9646

>NTDB_id=879614 RJD39_RS14460 WP_406732564.1 3067378..3068409(+) (pilT) [Vibrio scophthalmi strain 21FBVib0096]
MDIAELLDFSVKHNASDLHLSAGVSPMVRIDGEVRKLGIPAFSQAEVHRLIFEVMNDAQRSEFEEHLEVDFSFELPDIGR
FRVNAFNQARGSAAVFRTIPTSIPTLDQLEAPAIFESIVNLEKGLVLVTGPTGSGKSTTLAAMVDYINQNHNKHILTIED
PIEFVHSNQKCLVNQREVHRDTRSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFATLHTSSAAKTIDRI
IDVFPGSEKGMVRSMLSESLRSVIAQKLLKRIGGGRVACHEIMMATPAIRNLIREDKVAQMYSVIQTGSAQGMQTMEQNA
KLLIAKGLVDQAEVEQKIGSLDF

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=879614 RJD39_RS14460 WP_406732564.1 3067378..3068409(+) (pilT) [Vibrio scophthalmi strain 21FBVib0096]
ATGGATATCGCTGAGTTACTGGATTTTAGTGTAAAACATAATGCGTCAGATCTACATCTTTCTGCGGGTGTTTCTCCGAT
GGTGCGCATTGATGGCGAAGTTCGCAAGCTGGGAATCCCTGCTTTTAGCCAGGCCGAAGTCCATCGCTTGATTTTTGAAG
TGATGAACGATGCTCAGCGTAGCGAATTTGAAGAACACTTAGAAGTTGATTTTTCATTTGAACTTCCCGATATCGGTCGT
TTTCGTGTTAATGCGTTTAATCAAGCACGCGGCAGTGCAGCGGTTTTTCGTACTATTCCTACCTCTATTCCAACGTTGGA
TCAGCTTGAAGCGCCGGCGATATTTGAAAGTATCGTTAACTTGGAAAAAGGCTTAGTGCTGGTGACGGGCCCCACGGGTT
CTGGTAAGTCGACCACATTGGCGGCGATGGTTGATTATATTAATCAAAACCATAACAAGCATATTCTTACCATTGAAGAT
CCGATTGAATTTGTTCACAGCAATCAAAAATGTTTGGTCAACCAACGTGAAGTACATCGAGATACTCGCAGCTTTAAAGC
GGCGTTGCGTAGTGCTTTACGTGAAGATCCTGATGTCATTCTTGTTGGTGAGTTGCGTGATCAAGAAACGATTAGTTTGG
CGCTAACGGCAGCGGAAACGGGCCACTTAGTTTTTGCTACGTTGCACACCAGCTCTGCAGCCAAAACCATTGACCGTATT
ATTGATGTTTTTCCGGGCAGTGAGAAAGGCATGGTGCGTTCTATGCTTTCGGAATCACTACGTTCAGTGATTGCACAAAA
ACTGCTGAAGCGTATTGGCGGTGGTCGTGTTGCCTGTCATGAAATTATGATGGCGACGCCTGCAATCCGTAACTTGATCC
GTGAAGATAAAGTCGCGCAGATGTATTCAGTGATCCAAACAGGATCGGCTCAAGGCATGCAAACTATGGAACAAAATGCC
AAACTTTTGATTGCCAAGGGCTTGGTCGATCAAGCTGAGGTTGAGCAAAAAATTGGCTCGCTAGATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

86.095

98.542

0.848
  pilT Vibrio cholerae strain A1552

86.095

98.542

0.848
  pilT Pseudomonas aeruginosa PAK

67.953

98.251

0.668
  pilT Pseudomonas stutzeri DSM 10701

67.953

98.251

0.668
  pilT Acinetobacter baylyi ADP1

69.091

96.21

0.665
  pilT Acinetobacter baumannii strain A118

68.693

95.918

0.659
  pilT Acinetobacter baumannii D1279779

68.693

95.918

0.659
  pilT Acinetobacter nosocomialis M2

68.389

95.918

0.656
  pilT Legionella pneumophila strain Lp02

66.767

96.501

0.644
  pilT Legionella pneumophila strain ERS1305867

66.767

96.501

0.644
  pilT Neisseria meningitidis 8013

64.242

96.21

0.618
  pilT Neisseria gonorrhoeae MS11

63.939

96.21

0.615
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.572

92.711

0.478
  pilU Vibrio cholerae strain A1552

43.574

93.003

0.405
  pilU Pseudomonas stutzeri DSM 10701

41.768

95.627

0.399
  pilU Acinetobacter baylyi ADP1

40.312

93.294

0.376