Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RJD39_RS14465 Genome accession   NZ_CP134277
Coordinates   3068424..3069524 (+) Length   366 a.a.
NCBI ID   WP_406732566.1    Uniprot ID   -
Organism   Vibrio scophthalmi strain 21FBVib0096     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3063424..3074524
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJD39_RS14430 (RJD39_14410) - 3063809..3064405 (-) 597 WP_009385807.1 XTP/dITP diphosphatase -
  RJD39_RS14435 (RJD39_14415) - 3064418..3064849 (-) 432 WP_005595631.1 DUF4426 domain-containing protein -
  RJD39_RS14440 (RJD39_14420) yggU 3064880..3065170 (-) 291 WP_406732560.1 DUF167 family protein YggU -
  RJD39_RS14445 (RJD39_14425) - 3065170..3065727 (-) 558 WP_406732562.1 YggT family protein -
  RJD39_RS14450 (RJD39_14430) proC 3065769..3066587 (-) 819 WP_069445969.1 pyrroline-5-carboxylate reductase -
  RJD39_RS14455 (RJD39_14435) - 3066637..3067353 (-) 717 WP_009385811.1 YggS family pyridoxal phosphate-dependent enzyme -
  RJD39_RS14460 (RJD39_14440) pilT 3067378..3068409 (+) 1032 WP_406732564.1 type IV pilus twitching motility protein PilT Machinery gene
  RJD39_RS14465 (RJD39_14445) pilU 3068424..3069524 (+) 1101 WP_406732566.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RJD39_RS14470 (RJD39_14450) lysC 3069588..3070940 (-) 1353 WP_069445972.1 lysine-sensitive aspartokinase 3 -
  RJD39_RS14475 (RJD39_14455) - 3071405..3072526 (+) 1122 WP_406732567.1 pyridoxal-phosphate-dependent aminotransferase family protein -
  RJD39_RS14480 (RJD39_14460) - 3072605..3074374 (-) 1770 WP_406732568.1 PglL family O-oligosaccharyltransferase -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 40946.75 Da        Isoelectric Point: 5.8360

>NTDB_id=879615 RJD39_RS14465 WP_406732566.1 3068424..3069524(+) (pilU) [Vibrio scophthalmi strain 21FBVib0096]
MDAFLQGMSEQKASDLYITVGAPVLYRVDGELQPQGDKLGEQEVFALLRSLMDSERLNEFKRSREANFAVVRDCGRFRVS
AFFQREQPGAVIRRIETKIPTCEELNLPTVLQELATTKRGLVLVVGATGSGKSTTMAAMTGYRNLHRSGHILTVEDPIEF
VHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMAFAETGHLCMATLHANNANQALERILHLV
PKEKKEQFLFDLSMNLKGVVAQQLLRDKNGQGRHGVFEVLLNSPRIADLIRRGELHEVKATMAKSQQTGMQTFDQSLFQL
LVDGKISEEDALHSADSANDLRIMLKTERGESLTSGSLADITIDMD

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=879615 RJD39_RS14465 WP_406732566.1 3068424..3069524(+) (pilU) [Vibrio scophthalmi strain 21FBVib0096]
ATGGATGCCTTCCTTCAAGGAATGAGTGAGCAAAAAGCGTCAGATCTGTATATCACCGTTGGCGCTCCAGTGCTTTATCG
TGTTGATGGTGAATTACAGCCACAAGGCGATAAGCTTGGTGAGCAAGAGGTGTTCGCGTTATTGAGATCGTTGATGGATA
GCGAGCGCTTAAACGAGTTTAAACGCAGTCGTGAAGCGAATTTTGCCGTGGTGCGAGATTGCGGACGATTTCGTGTTTCT
GCCTTTTTTCAACGTGAGCAACCTGGGGCGGTAATTCGACGTATCGAAACGAAGATTCCAACTTGTGAAGAACTTAATCT
CCCTACGGTGTTGCAAGAGTTGGCGACCACCAAACGAGGTTTGGTGCTGGTGGTTGGTGCAACCGGTTCTGGTAAGTCGA
CTACCATGGCGGCAATGACCGGCTATCGAAATCTACACCGCAGCGGTCATATTCTCACCGTGGAAGATCCGATTGAATTT
GTTCACGAACATCAGCGCTGCATTGTGACTCAGCGTGAAGTCGGCCTCGATACTGAAAGCTATGAAGTCGCCCTGAAAAA
CTCGCTGCGCCAAGCCCCGGATATGATCTTAATTGGTGAGATTCGCAGTCGAGAAACCATGGAATATGCGATGGCGTTTG
CGGAAACCGGCCATCTGTGTATGGCAACCTTGCACGCTAATAATGCCAATCAAGCGTTAGAGCGTATTTTACATTTGGTG
CCCAAAGAGAAAAAAGAGCAGTTCTTATTTGATTTGTCGATGAACCTGAAAGGCGTGGTTGCTCAGCAGTTGCTGCGAGA
TAAAAATGGTCAAGGTCGTCATGGTGTTTTTGAAGTGCTGCTCAATAGCCCTCGCATCGCTGATTTGATCCGTCGTGGCG
AGTTGCACGAAGTAAAAGCGACCATGGCGAAATCGCAGCAGACGGGTATGCAGACTTTTGACCAGTCGTTATTTCAGTTG
CTGGTTGATGGCAAAATTAGTGAAGAGGATGCGCTGCACAGTGCTGACTCTGCCAATGATTTACGCATTATGCTTAAAAC
GGAGCGCGGAGAGTCTTTAACGAGTGGATCTCTGGCTGATATCACCATTGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

81.967

100

0.82
  pilU Pseudomonas stutzeri DSM 10701

57.459

98.907

0.568
  pilU Acinetobacter baylyi ADP1

54.335

94.536

0.514
  pilT Legionella pneumophila strain Lp02

42.683

89.617

0.383
  pilT Legionella pneumophila strain ERS1305867

42.683

89.617

0.383
  pilT Acinetobacter baylyi ADP1

42.724

88.251

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.265

90.71

0.374
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.641

89.891

0.374
  pilT Pseudomonas aeruginosa PAK

41.337

89.891

0.372
  pilT Acinetobacter baumannii strain A118

41.231

88.798

0.366
  pilT Acinetobacter nosocomialis M2

41.231

88.798

0.366
  pilT Acinetobacter baumannii D1279779

41.231

88.798

0.366