Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RI845_RS14675 Genome accession   NZ_CP134146
Coordinates   3399112..3400155 (-) Length   347 a.a.
NCBI ID   WP_348386917.1    Uniprot ID   -
Organism   Thalassotalea nanhaiensis strain SQ345     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3394112..3405155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI845_RS14660 (RI845_14660) - 3395793..3396980 (-) 1188 WP_348386914.1 acyl-CoA dehydrogenase -
  RI845_RS14665 (RI845_14665) - 3397134..3397970 (+) 837 WP_348386915.1 LysR family transcriptional regulator -
  RI845_RS14670 (RI845_14670) pilU 3397986..3399098 (-) 1113 WP_348386916.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI845_RS14675 (RI845_14675) pilT 3399112..3400155 (-) 1044 WP_348386917.1 type IV pilus twitching motility protein PilT Machinery gene
  RI845_RS14680 (RI845_14680) - 3400192..3400875 (+) 684 WP_348386918.1 YggS family pyridoxal phosphate-dependent enzyme -
  RI845_RS14685 (RI845_14685) proC 3400947..3401762 (+) 816 WP_348386919.1 pyrroline-5-carboxylate reductase -
  RI845_RS14690 (RI845_14690) - 3401837..3402385 (+) 549 WP_348386920.1 YggT family protein -
  RI845_RS14695 (RI845_14695) - 3402478..3402921 (+) 444 WP_348386921.1 DUF4426 domain-containing protein -
  RI845_RS14700 (RI845_14700) - 3403019..3403609 (+) 591 WP_348386922.1 XTP/dITP diphosphatase -
  RI845_RS14705 (RI845_14705) hemW 3403630..3404787 (+) 1158 WP_348386923.1 radical SAM family heme chaperone HemW -
  RI845_RS14710 (RI845_14710) - 3404811..3405029 (-) 219 WP_348386924.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38822.39 Da        Isoelectric Point: 5.6234

>NTDB_id=878825 RI845_RS14675 WP_348386917.1 3399112..3400155(-) (pilT) [Thalassotalea nanhaiensis strain SQ345]
MDITELLAFSVEHNASDLHLSTGMPPAIRVDGDVRKVNIPPLEDKDVNGLVYDIMTDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKILSLDELGCPDIFRQISENPRGLVLVTGPTGSGKSTTLAAMVDYINESKRDHILTIED
PIEFVHDNKQCLINQREVHRDTLSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGEEKDMVRSMLSESLRAVISQTLIKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLLSRGLITREDAMHKAADKNQFKVY

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=878825 RI845_RS14675 WP_348386917.1 3399112..3400155(-) (pilT) [Thalassotalea nanhaiensis strain SQ345]
ATGGATATTACCGAACTTTTAGCATTTAGTGTTGAACACAATGCTTCCGATTTACATTTATCTACTGGTATGCCACCGGC
CATTCGTGTTGATGGTGACGTACGTAAGGTTAACATCCCGCCGTTAGAAGACAAAGACGTTAATGGTTTAGTGTATGACA
TTATGACCGATCGTCAACGAAAAGAGTACGAAGAAAACCTAGAAGTGGATTTCTCTTTTGAAGTACCAAACTTAGCTCGT
TTTCGTGTTAACGCATTCAATCAAAATCGCGGCCCAGCGGCGGTATTTCGTACCATCCCAAGTAAGATTTTATCTCTTGA
TGAGTTGGGCTGTCCTGATATATTTCGTCAAATATCAGAAAACCCACGTGGACTGGTATTAGTAACCGGTCCAACAGGTT
CGGGTAAATCAACAACGTTGGCGGCGATGGTCGATTACATTAATGAATCAAAACGCGATCACATACTAACCATTGAAGAT
CCAATCGAATTTGTTCATGACAATAAACAGTGTTTAATTAACCAACGTGAAGTACATCGCGATACATTAAGCTTTAACAA
CGCATTACGCTCGGCGCTTCGTGAAGATCCAGATGTAATACTTGTTGGTGAAATGCGTGATTTGGAAACCATTCGCCTGG
CAATGACCGCGGCAGAAACTGGTCACTTAGTATTTGGTACCCTACATACTACCTCTGCACCAAAAACCATTGACCGTATT
GTTGATGTATTCCCTGGTGAAGAAAAAGACATGGTTCGCTCCATGTTATCAGAATCACTTCGTGCGGTAATATCGCAAAC
GCTAATCAAAAAAGTTGGCGGTGGTCGAGTCGCTGCCCATGAAATCATGATCGGTGTACCTGCCATTCGTAACTTAATTC
GTGAAGATAAAATTGCGCAAATGTATTCTGCAATTCAAACCGGCATGTCACACGGCATGCAAACCATGGATCAATGTTTA
CAAAACTTATTAAGCCGTGGCCTTATCACCCGTGAAGATGCAATGCATAAAGCAGCGGATAAAAACCAGTTTAAGGTTTA
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.744

99.135

0.761
  pilT Acinetobacter nosocomialis M2

76.453

99.135

0.758
  pilT Acinetobacter baumannii strain A118

76.163

99.135

0.755
  pilT Acinetobacter baumannii D1279779

76.163

99.135

0.755
  pilT Pseudomonas stutzeri DSM 10701

75.872

99.135

0.752
  pilT Acinetobacter baylyi ADP1

75.291

99.135

0.746
  pilT Legionella pneumophila strain Lp02

72.674

99.135

0.72
  pilT Legionella pneumophila strain ERS1305867

72.674

99.135

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.92

97.118

0.689
  pilT Vibrio cholerae strain A1552

70.92

97.118

0.689
  pilT Neisseria meningitidis 8013

69.208

98.271

0.68
  pilT Neisseria gonorrhoeae MS11

68.915

98.271

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

99.424

0.53
  pilU Vibrio cholerae strain A1552

40.922

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.346

100

0.403
  pilU Acinetobacter baylyi ADP1

39.655

100

0.398