Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QQE96_RS18965 Genome accession   NZ_CP133987
Coordinates   2930619..2931656 (-) Length   345 a.a.
NCBI ID   WP_053308737.1    Uniprot ID   -
Organism   Vibrio aestuarianus subsp. francensis strain LC_Sco     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2925619..2936656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQE96_RS18935 (QQE96_18990) endA 2925865..2926569 (+) 705 WP_168523447.1 deoxyribonuclease I -
  QQE96_RS18940 (QQE96_18995) rsmE 2926677..2927408 (+) 732 WP_168523448.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QQE96_RS18945 (QQE96_19000) gshB 2927422..2928369 (+) 948 WP_053308733.1 glutathione synthase -
  QQE96_RS18950 (QQE96_19005) - 2928423..2928986 (+) 564 WP_053308734.1 YqgE/AlgH family protein -
  QQE96_RS18955 (QQE96_19010) ruvX 2929006..2929434 (+) 429 WP_053308735.1 Holliday junction resolvase RuvX -
  QQE96_RS18960 (QQE96_19015) pilU 2929502..2930608 (-) 1107 WP_168523450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QQE96_RS18965 (QQE96_19020) pilT 2930619..2931656 (-) 1038 WP_053308737.1 type IV pilus twitching motility protein PilT Machinery gene
  QQE96_RS18970 (QQE96_19025) - 2931682..2932389 (+) 708 WP_053308738.1 YggS family pyridoxal phosphate-dependent enzyme -
  QQE96_RS18975 (QQE96_19030) proC 2932422..2933240 (+) 819 WP_168523452.1 pyrroline-5-carboxylate reductase -
  QQE96_RS18980 (QQE96_19035) - 2933303..2933860 (+) 558 WP_053308740.1 YggT family protein -
  QQE96_RS18985 (QQE96_19040) yggU 2933860..2934150 (+) 291 WP_053308741.1 DUF167 family protein YggU -
  QQE96_RS18990 (QQE96_19045) - 2934193..2934624 (+) 432 WP_168523454.1 DUF4426 domain-containing protein -
  QQE96_RS18995 (QQE96_19050) - 2934640..2935236 (+) 597 WP_053308743.1 XTP/dITP diphosphatase -
  QQE96_RS19000 (QQE96_19055) hemW 2935239..2936399 (+) 1161 WP_168523456.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38179.66 Da        Isoelectric Point: 6.2274

>NTDB_id=878125 QQE96_RS18965 WP_053308737.1 2930619..2931656(-) (pilT) [Vibrio aestuarianus subsp. francensis strain LC_Sco]
MDIAELLDFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHADVHRLIFEIMNDAQRSEFEEKLEVDFSFELPDVGR
FRVNAFHQSRGCSAVFRTIPTTIPTLEELQTPDIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVDYINTHHNKHILTIED
PIEFVHSNNKCLVNQREVHRDTHSFKAALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKEMVRSMLSESLRAVIAQKLLKRIGGGRVACHEIMLGTPAIRNLIREDKVAQMFSIIQTGAAHGMQTMEQNA
KQLIAQGIVDAEEVQTKLELNSSPF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=878125 QQE96_RS18965 WP_053308737.1 2930619..2931656(-) (pilT) [Vibrio aestuarianus subsp. francensis strain LC_Sco]
ATGGATATCGCTGAGCTACTGGATTTTAGTGTAAAGCACAACGCGTCAGATCTGCATCTTTCTGCAGGTGTTCCACCTAT
GGTACGTATAGATGGTGAAGTAAGAAAGCTTGGAGTGCCAGCTTTTACTCATGCAGATGTACATCGTTTGATTTTTGAGA
TCATGAACGATGCTCAACGTAGTGAATTTGAAGAGAAATTGGAAGTCGATTTTTCGTTTGAGTTACCCGATGTCGGACGT
TTTCGTGTCAACGCGTTTCATCAATCTCGTGGCTGCTCTGCGGTTTTTCGTACAATTCCAACCACAATTCCGACATTAGA
AGAGCTGCAAACGCCAGATATTTTCAGCAAAATTGCCAACTACGAAAAGGGATTGGTACTGGTAACCGGACCTACAGGGT
CAGGCAAGTCAACCACGCTAGCGGCGATGGTCGATTACATTAATACTCATCACAACAAGCATATTTTGACGATTGAAGAT
CCGATTGAATTTGTGCATAGCAACAATAAATGTTTGGTCAATCAACGAGAAGTACACCGCGATACACACAGTTTCAAAGC
GGCTTTGCGCTCGGCATTGCGTGAAGACCCAGACGTTATTTTGGTCGGTGAATTACGTGACCAAGAAACCATTAGCTTAG
CGTTAACCGCGGCGGAAACTGGCCACTTAGTGTTTGGCACTTTGCACACTAGCTCTGCGGCGAAAACCATTGATCGAATT
ATTGATGTGTTTCCCGGCAGCGATAAAGAGATGGTGCGTTCTATGCTGTCAGAGTCATTACGTGCGGTGATTGCGCAAAA
ACTGCTAAAACGAATTGGTGGTGGCCGAGTTGCTTGTCATGAAATTATGTTGGGGACGCCTGCCATTCGTAACCTGATTC
GCGAAGATAAAGTTGCTCAGATGTTTTCCATTATTCAGACTGGAGCGGCTCATGGGATGCAAACCATGGAACAAAATGCG
AAGCAGCTCATTGCTCAAGGTATAGTTGACGCTGAAGAAGTGCAAACAAAGTTAGAATTGAACTCATCCCCGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

91.884

100

0.919
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

91.884

100

0.919
  pilT Acinetobacter baylyi ADP1

72.121

95.652

0.69
  pilT Acinetobacter baumannii D1279779

70.536

97.391

0.687
  pilT Acinetobacter baumannii strain A118

70.536

97.391

0.687
  pilT Acinetobacter nosocomialis M2

70.238

97.391

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

68.235

98.551

0.672
  pilT Legionella pneumophila strain Lp02

66.377

100

0.664
  pilT Legionella pneumophila strain ERS1305867

66.377

100

0.664
  pilT Neisseria meningitidis 8013

62.974

99.42

0.626
  pilT Neisseria gonorrhoeae MS11

62.059

98.551

0.612
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.403

92.174

0.501
  pilU Vibrio cholerae strain A1552

44.923

94.203

0.423
  pilU Pseudomonas stutzeri DSM 10701

42.308

97.971

0.414
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377