Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QQE96_RS18960 Genome accession   NZ_CP133987
Coordinates   2929502..2930608 (-) Length   368 a.a.
NCBI ID   WP_168523450.1    Uniprot ID   -
Organism   Vibrio aestuarianus subsp. francensis strain LC_Sco     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2924502..2935608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQE96_RS18930 (QQE96_18985) - 2925240..2925740 (+) 501 WP_168523444.1 SprT family zinc-dependent metalloprotease -
  QQE96_RS18935 (QQE96_18990) endA 2925865..2926569 (+) 705 WP_168523447.1 deoxyribonuclease I -
  QQE96_RS18940 (QQE96_18995) rsmE 2926677..2927408 (+) 732 WP_168523448.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QQE96_RS18945 (QQE96_19000) gshB 2927422..2928369 (+) 948 WP_053308733.1 glutathione synthase -
  QQE96_RS18950 (QQE96_19005) - 2928423..2928986 (+) 564 WP_053308734.1 YqgE/AlgH family protein -
  QQE96_RS18955 (QQE96_19010) ruvX 2929006..2929434 (+) 429 WP_053308735.1 Holliday junction resolvase RuvX -
  QQE96_RS18960 (QQE96_19015) pilU 2929502..2930608 (-) 1107 WP_168523450.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QQE96_RS18965 (QQE96_19020) pilT 2930619..2931656 (-) 1038 WP_053308737.1 type IV pilus twitching motility protein PilT Machinery gene
  QQE96_RS18970 (QQE96_19025) - 2931682..2932389 (+) 708 WP_053308738.1 YggS family pyridoxal phosphate-dependent enzyme -
  QQE96_RS18975 (QQE96_19030) proC 2932422..2933240 (+) 819 WP_168523452.1 pyrroline-5-carboxylate reductase -
  QQE96_RS18980 (QQE96_19035) - 2933303..2933860 (+) 558 WP_053308740.1 YggT family protein -
  QQE96_RS18985 (QQE96_19040) yggU 2933860..2934150 (+) 291 WP_053308741.1 DUF167 family protein YggU -
  QQE96_RS18990 (QQE96_19045) - 2934193..2934624 (+) 432 WP_168523454.1 DUF4426 domain-containing protein -
  QQE96_RS18995 (QQE96_19050) - 2934640..2935236 (+) 597 WP_053308743.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41329.24 Da        Isoelectric Point: 6.2350

>NTDB_id=878124 QQE96_RS18960 WP_168523450.1 2929502..2930608(-) (pilU) [Vibrio aestuarianus subsp. francensis strain LC_Sco]
MELNQCLEGMLNQKASDLYITVGAPILFRVDGELRAQGEKLTTPQVIDLLDAMMDSERREEFKHTHEANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFDDLKLPGVLQDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPQEQKDQLLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGELHELKATMGKSQQVGMQTFDQALY
QLVIDGKICEQDALHSADSANDLRLMLKTKRGDRYGSGSLDNVQIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=878124 QQE96_RS18960 WP_168523450.1 2929502..2930608(-) (pilU) [Vibrio aestuarianus subsp. francensis strain LC_Sco]
ATGGAATTAAATCAATGCCTTGAGGGCATGTTAAATCAAAAAGCATCGGATCTTTATATTACGGTTGGTGCTCCGATTTT
GTTTCGAGTTGATGGCGAATTGAGAGCGCAAGGTGAAAAGCTAACCACACCACAAGTGATTGATTTGCTCGATGCAATGA
TGGATAGCGAACGCCGTGAAGAGTTTAAGCACACTCATGAAGCTAACTTTGCGATTGTGCGTGATTTTGGGCGTTTTCGT
GTCAGCGCCTTTTTTCAACGTGAATTACCGGGAGCGGTGATCCGTCGTATTGAAACCAATATTCCAACCTTTGATGACCT
AAAGTTACCGGGAGTTTTACAGGATCTGTCGATTGCAAAGCGTGGTTTGGTTCTGGTCGTTGGGGCTACGGGGTCGGGTA
AATCGACCACGATGGCTGCGATGACGGGGTATCGCAATACGCATCGAAGCGGGCATATTTTAACGGTGGAAGATCCGATC
GAGTTTGTGCATGAGCATCAACGCTGCATCGTCACGCAGCGTGAAGTTGGGTTAGATACCGAAAGCTACGAGGTGGCGCT
GAAGAATTCACTTCGACAGGCGCCTGATATGATTTTGATTGGCGAAATACGTTCTCGAGAAACCATGCAGTATGCGATGA
CCTTTGCTGAAACGGGCCATTTATGCATGGCAACGTTACACGCTAATAATGCCAACCAAGCGTTAGAGCGAATTCTTCAT
TTGGTTCCTCAAGAGCAAAAAGATCAGCTTTTGTTTGATCTTTCAATGAACCTGAAAGGGGTGATTGGCCAGCAGTTGAT
CCGCGATAAAAACGGACAAGGGCGACATGGTGTGTTTGAGATCCTACTCAATAGTCCGCGCGTTTCTGATTTGATCCGCC
GCGGTGAACTGCACGAACTGAAAGCCACCATGGGGAAATCACAACAGGTAGGTATGCAGACATTTGATCAAGCGCTTTAC
CAACTAGTGATTGATGGCAAGATCTGCGAGCAAGACGCACTTCATAGTGCCGATTCAGCCAATGATTTACGTTTGATGCT
CAAAACCAAACGCGGTGACCGTTATGGATCTGGCTCATTAGATAATGTTCAGATTGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

100

0.856
  pilU Pseudomonas stutzeri DSM 10701

58.286

95.109

0.554
  pilU Acinetobacter baylyi ADP1

54.701

95.38

0.522
  pilT Legionella pneumophila strain Lp02

41.399

93.207

0.386
  pilT Legionella pneumophila strain ERS1305867

41.399

93.207

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

88.043

0.38
  pilT Acinetobacter baumannii D1279779

41.298

92.12

0.38
  pilT Pseudomonas aeruginosa PAK

41.298

92.12

0.38
  pilT Acinetobacter nosocomialis M2

41.298

92.12

0.38
  pilT Acinetobacter baumannii strain A118

41.298

92.12

0.38
  pilT Acinetobacter baylyi ADP1

41.617

90.761

0.378
  pilT Pseudomonas stutzeri DSM 10701

40.233

93.207

0.375
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372