Detailed information    

experimental Experimentally validated

Overview

Name   recX   Type   Regulator
Locus tag   EQG65_RS09765 Genome accession   NZ_CP035101
Coordinates   1938340..1939158 (-) Length   272 a.a.
NCBI ID   WP_001124419.1    Uniprot ID   Q8NVU3
Organism   Staphylococcus aureus strain ATCC 12600     
Function   repressor of recA   
Homologous recombination

Function

RecX protein is an antirecombinase during HR and play a pivotal role in regulation of RecA during the DNA transactions.


Genomic Context

Location: 1933340..1944158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EQG65_RS09740 (EQG65_09965) - 1934433..1935410 (+) 978 WP_000379098.1 metal-dependent hydrolase -
  EQG65_RS09750 (EQG65_09975) - 1935671..1936507 (-) 837 WP_000886468.1 membrane protein -
  EQG65_RS09755 (EQG65_09980) - 1936516..1938033 (-) 1518 WP_000535834.1 ABC transporter ATP-binding protein -
  EQG65_RS09760 (EQG65_09985) - 1938048..1938362 (-) 315 WP_000435806.1 YfhH family protein -
  EQG65_RS09765 (EQG65_09990) recX 1938340..1939158 (-) 819 WP_001124419.1 recombination regulator RecX Regulator
  EQG65_RS09770 (EQG65_09995) sgtB 1939419..1940228 (-) 810 WP_000830380.1 monofunctional peptidoglycan glycosyltransferase SgtB -
  EQG65_RS09775 (EQG65_10000) - 1940569..1941084 (-) 516 WP_000163283.1 type 1 glutamine amidotransferase domain-containing protein -
  EQG65_RS09780 (EQG65_10005) - 1941215..1941376 (-) 162 WP_001005407.1 SE1561 family protein -
  EQG65_RS09785 (EQG65_10010) yfkAB 1941600..1942751 (+) 1152 WP_001089613.1 radical SAM/CxCxxxxC motif protein YfkAB -
  EQG65_RS09790 (EQG65_10015) - 1943011..1943541 (-) 531 WP_000184383.1 acyl-CoA thioesterase -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  recX recA negative effect

Sequence

Protein


Download         Length: 272 a.a.        Molecular weight: 32241.06 Da        Isoelectric Point: 5.9211

>NTDB_id=673 EQG65_RS09765 WP_001124419.1 1938340..1939158(-) (recX) [Staphylococcus aureus strain ATCC 12600]
MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQLEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEV
IQYLQKEEISEQAISEVIEYCYREKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEIFTELYREQQELDD
IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQRDLEKIYNKNRKKYTQQKLISK
TIEGLMRKGYKYDKIKAKLEESGIADGTEEIE

Nucleotide


Download         Length: 819 bp        

>NTDB_id=673 EQG65_RS09765 WP_001124419.1 1938340..1939158(-) (recX) [Staphylococcus aureus strain ATCC 12600]
GTGCCGAAGATTACTAAAATAGAAGTTCAAAAGAAAAACAAAGAACGGTTTAATCTTTTTTTAGACGAACAATTTGAAAT
GGGTATAGATATTGATACATTAGTCAAATTTAATTTAAAAAAAGGGCAACAACTTGAAGCTGCTGACATGGCAGAGATTC
AAAAGTATGATCATTATCGCATAGGTTTAAATAAAGCAATCCAATATTTATCATATAAAAAGAGAACTGAAAAAGAAGTT
ATACAATATTTACAAAAAGAAGAGATATCAGAGCAAGCGATTTCTGAAGTGATTGAATATTGTTATCGCGAAAAGTTAAT
CGACCATCAAGATTATGCGGAAAGTTTAAAAAATACAATGATTCGCACGACAGATAAAGGACCTAAAATTTATCAACAAA
AACTATATCAACTTGGTATAGAACCAAATATCATTGAAATATTTACAGAACTTTATAGAGAACAACAGGAACTTGATGAT
ATTATCCAAATTGCTGAAAAAATATCTAAAACAAAAAAAGGGCCACAAAATAAAGTTAAAGAAAAAGTAATGCAATCTTT
AATTCAAAAAGGGTTTGAAATGGAAACCATTCATGCTGTATTGAATGAAATGGATTTTACACAAGATGAAGCGGTTTTAG
ACGATTTATTACAACGAGATTTAGAAAAAATTTATAATAAAAATCGAAAGAAGTACACGCAACAGAAATTAATTTCAAAA
ACTATCGAAGGCCTTATGAGAAAAGGATATAAATATGATAAAATTAAAGCTAAATTAGAAGAAAGTGGTATTGCCGATGG
AACAGAAGAAATTGAGTGA

Domains

Predicted by InterProScan.

RecX family (RecX_HTH2)

(84-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8NVU3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Kajal Kiran et al. (2023) Characterization of Staphylococcus aureus RecX protein: Molecular insights into negative regulation of RecA protein and implications in HR processes. Journal of Biochemistry 174(3):227-237. [PMID: 37115499]