Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RI210_RS22270 Genome accession   NZ_CP133985
Coordinates   4939272..4940309 (-) Length   345 a.a.
NCBI ID   WP_054759731.1    Uniprot ID   -
Organism   Methylomonas koyamae strain MY1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4934272..4945309
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI210_RS22245 (RI210_22245) - 4934596..4935231 (-) 636 WP_310751201.1 hypothetical protein -
  RI210_RS22250 (RI210_22250) - 4935259..4935429 (-) 171 WP_310751204.1 hypothetical protein -
  RI210_RS22255 (RI210_22255) - 4935487..4936080 (-) 594 WP_310751207.1 recombinase family protein -
  RI210_RS22260 (RI210_22260) mltB 4936926..4938104 (-) 1179 WP_310751210.1 lytic murein transglycosylase B -
  RI210_RS22265 (RI210_22265) pilU 4938122..4939255 (-) 1134 WP_064023282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI210_RS22270 (RI210_22270) pilT 4939272..4940309 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  RI210_RS22275 (RI210_22275) - 4940446..4941468 (-) 1023 WP_310751213.1 SPFH domain-containing protein -
  RI210_RS22280 (RI210_22280) - 4941668..4942291 (+) 624 WP_064027612.1 TPM domain-containing protein -
  RI210_RS22285 (RI210_22285) - 4942288..4943796 (+) 1509 WP_310751215.1 TIM44-like domain-containing protein -
  RI210_RS22290 (RI210_22290) - 4943941..4944294 (-) 354 WP_064023286.1 YciI family protein -
  RI210_RS22295 (RI210_22295) - 4944291..4944803 (-) 513 WP_310751218.1 SRPBCC family protein -
  RI210_RS22300 (RI210_22300) - 4944826..4945296 (-) 471 WP_310751221.1 VOC family protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38324.18 Da        Isoelectric Point: 7.0362

>NTDB_id=878086 RI210_RS22270 WP_054759731.1 4939272..4940309(-) (pilT) [Methylomonas koyamae strain MY1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALNHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELDAPKFFQELCTKPRGLILVTGPTGSGKSTTLAAMINHINNNDYAHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRAMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDKGLITAKAAMTRAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=878086 RI210_RS22270 WP_054759731.1 4939272..4940309(-) (pilT) [Methylomonas koyamae strain MY1]
ATGGATATCGCCGAACTGCTGACGTTTTCCGTCAAGAATAAAGCCTCCGACTTGCACCTTTCCGCCGGCTTGCCGCCGAT
GATACGGGTGGACGGCGATATTCGCCGGATCAACATCCCGGCGCTGAACCATAAGGAAGTGCATGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGCCGCGACTACGAGGAGTTTCTGGAAACCGACTTTTCCTTCGCCTTGCCCGGCGTGGCCCGG
TTCCGGGTCAACGCCTTCAACCAGGACCGCGGCGCCGGCGCGGTATTCCGCACCATTCCGTCGAAAGTCCTGACCTTGGA
AGAGCTGGATGCACCCAAGTTTTTCCAGGAATTGTGTACCAAACCGCGCGGCTTGATCCTGGTGACCGGACCGACCGGTT
CCGGTAAATCGACCACGTTGGCGGCGATGATCAACCACATCAACAATAACGATTATGCCCACATCCTGACCGTCGAAGAC
CCGATCGAGTTCGTCCACGAAAGCCAGAAGTCGCTGATCAACCAGCGCGAAGTGCACCGCGACACCCTGGGTTTCAACGA
AGCGTTGCGTTCCGCGTTGCGGGAAGACCCCGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATTCGTTTGG
CGCTGACCGCGGCCGAAACCGGCCACTTGGTGTTCGGCACTCTGCACACCACCTCGGCCGCCAAGACCATAGACCGGATC
ATCGACGTGTTCCCGGCGGCCGAGAAAGACATGATCCGCGCCATGCTATCGGAATCGTTGCAAGCGGTTATATCGCAAAC
GCTGTTGAAGAAAGTCGGCGGCGGCCGGATCGCTGCGCACGAGATCATGGTCGGTACCCCGGCGATCCGCAACCTGATCC
GCGAAGCCAAGGTGGCACAGATGTATTCGGCGATTCAAACCGGGCGCAAGGACGGCATGCAGACGCTGGACCAAAATTTA
AAAGAGTTGGTCGACAAGGGCCTGATTACCGCCAAAGCGGCGATGACCCGAGCCGTCAATAAAGACATGTTCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

81.232

98.841

0.803
  pilT Acinetobacter baumannii D1279779

80.233

99.71

0.8
  pilT Acinetobacter baumannii strain A118

80.233

99.71

0.8
  pilT Acinetobacter nosocomialis M2

80.233

99.71

0.8
  pilT Pseudomonas stutzeri DSM 10701

79.07

99.71

0.788
  pilT Pseudomonas aeruginosa PAK

80.473

97.971

0.788
  pilT Legionella pneumophila strain Lp02

72.674

99.71

0.725
  pilT Legionella pneumophila strain ERS1305867

72.674

99.71

0.725
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.436

97.681

0.678
  pilT Vibrio cholerae strain A1552

69.436

97.681

0.678
  pilT Neisseria gonorrhoeae MS11

67.638

99.42

0.672
  pilT Neisseria meningitidis 8013

67.638

99.42

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Acinetobacter baylyi ADP1

40.346

100

0.406
  pilU Vibrio cholerae strain A1552

40.708

98.261

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4