Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RI210_RS22265 Genome accession   NZ_CP133985
Coordinates   4938122..4939255 (-) Length   377 a.a.
NCBI ID   WP_064023282.1    Uniprot ID   A0A172UEK3
Organism   Methylomonas koyamae strain MY1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4933122..4944255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RI210_RS22240 (RI210_22240) - 4933419..4934507 (+) 1089 WP_310747873.1 IS5 family transposase -
  RI210_RS22245 (RI210_22245) - 4934596..4935231 (-) 636 WP_310751201.1 hypothetical protein -
  RI210_RS22250 (RI210_22250) - 4935259..4935429 (-) 171 WP_310751204.1 hypothetical protein -
  RI210_RS22255 (RI210_22255) - 4935487..4936080 (-) 594 WP_310751207.1 recombinase family protein -
  RI210_RS22260 (RI210_22260) mltB 4936926..4938104 (-) 1179 WP_310751210.1 lytic murein transglycosylase B -
  RI210_RS22265 (RI210_22265) pilU 4938122..4939255 (-) 1134 WP_064023282.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RI210_RS22270 (RI210_22270) pilT 4939272..4940309 (-) 1038 WP_054759731.1 type IV pilus twitching motility protein PilT Machinery gene
  RI210_RS22275 (RI210_22275) - 4940446..4941468 (-) 1023 WP_310751213.1 SPFH domain-containing protein -
  RI210_RS22280 (RI210_22280) - 4941668..4942291 (+) 624 WP_064027612.1 TPM domain-containing protein -
  RI210_RS22285 (RI210_22285) - 4942288..4943796 (+) 1509 WP_310751215.1 TIM44-like domain-containing protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 42058.29 Da        Isoelectric Point: 6.6668

>NTDB_id=878085 RI210_RS22265 WP_064023282.1 4938122..4939255(-) (pilU) [Methylomonas koyamae strain MY1]
MDFTALLALMVEKRASDLFITAGRPPSMKVNGKVVEVSKTPLTSDQTMKLVLGIMSQRQRDEFENTKECQFALSAHSLGR
FRVSAFTQRDSAGMVLRRIETTIPTAEELHLPPVLKELIMHKRGLVMFVGATGTGKSTSLAALIRHRNENSSGHIISIED
PIEFIHPHQGCIITQREVGLDTESFEVALKNTLRQAPDVILIGEVRTRETMQHAITFAETGHLCVCTLHANNANQALDRI
LHFFPEDMHSQLFMDLSLNLRGIVAQQLIQRADGKGRYPAIEILLNTPLVADLVRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALHAADSRNEVRLMIKLGAENVNFETAGMSLAENDEDGGSLYK

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=878085 RI210_RS22265 WP_064023282.1 4938122..4939255(-) (pilU) [Methylomonas koyamae strain MY1]
ATGGACTTTACCGCGCTATTGGCATTGATGGTGGAAAAACGGGCTTCGGACTTGTTCATCACCGCCGGGCGGCCGCCGTC
GATGAAAGTCAACGGCAAAGTCGTCGAAGTCTCGAAAACGCCGCTGACCAGCGACCAAACCATGAAACTGGTACTCGGCA
TCATGTCGCAGCGCCAGCGCGACGAATTCGAAAACACCAAGGAATGCCAATTTGCATTAAGCGCGCACAGCCTGGGCCGA
TTCCGGGTCAGCGCCTTTACCCAACGCGATTCCGCCGGCATGGTGTTGCGCCGCATCGAAACCACGATTCCGACCGCGGA
AGAACTGCATCTGCCGCCGGTGTTGAAAGAATTGATCATGCACAAGCGCGGCCTGGTGATGTTCGTCGGCGCGACCGGTA
CCGGTAAATCGACCTCATTGGCGGCCTTGATTCGCCACCGCAACGAAAACAGCAGCGGCCACATCATTTCCATCGAAGAT
CCGATCGAGTTCATCCATCCGCACCAGGGCTGCATCATTACCCAGCGCGAAGTGGGGCTGGATACCGAATCGTTCGAAGT
GGCTTTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATTCTGATCGGCGAGGTTCGAACCCGCGAGACGATGCAACACG
CGATTACCTTCGCCGAGACCGGCCATCTCTGCGTCTGCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGGATT
TTACATTTCTTCCCCGAAGACATGCACAGCCAGTTGTTCATGGATTTGTCGCTGAATCTGCGCGGGATCGTGGCCCAGCA
ATTGATTCAACGCGCCGACGGCAAGGGCCGCTATCCGGCCATCGAAATTCTGTTGAATACGCCGTTGGTCGCCGACCTGG
TGCGCAAAGGCGAAGTGCATAAATTGAAGGAGCTGATGAAAAACTCGCGCGAGCATGGCATGCAGACTTTCGACCAGGCG
CTTTACGATCTGTACACAGCCGGTAAAATTAGTTACGAAGATGCTCTACACGCTGCCGACTCCCGCAACGAAGTCCGCCT
GATGATCAAACTGGGGGCCGAGAACGTCAATTTCGAAACCGCCGGCATGTCGCTGGCCGAGAACGACGAAGACGGAGGCA
GCCTTTATAAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172UEK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.241

100

0.69
  pilU Acinetobacter baylyi ADP1

64.225

94.164

0.605
  pilU Vibrio cholerae strain A1552

57.429

92.838

0.533
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.253

92.308

0.408
  pilT Legionella pneumophila strain ERS1305867

41.888

89.92

0.377
  pilT Legionella pneumophila strain Lp02

41.888

89.92

0.377
  pilT Pseudomonas aeruginosa PAK

41.003

89.92

0.369
  pilT Acinetobacter nosocomialis M2

40.413

89.92

0.363
  pilT Acinetobacter baumannii D1279779

40.413

89.92

0.363
  pilT Acinetobacter baumannii strain A118

40.413

89.92

0.363
  pilT Acinetobacter baylyi ADP1

39.883

90.451

0.361