Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PszF2a_RS20125 Genome accession   NZ_AP024722
Coordinates   4279201..4280235 (-) Length   344 a.a.
NCBI ID   WP_221292731.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2a     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4274201..4285235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PszF2a_RS20105 (PszF2a_39390) - 4275421..4275816 (-) 396 WP_011914966.1 NINE protein -
  PszF2a_RS20110 (PszF2a_39400) - 4275952..4277166 (+) 1215 WP_221292729.1 putative DNA modification/repair radical SAM protein -
  PszF2a_RS20115 (PszF2a_39410) - 4277166..4277999 (+) 834 WP_221292730.1 TIGR03915 family putative DNA repair protein -
  PszF2a_RS20120 (PszF2a_39420) pilU 4277996..4279141 (-) 1146 WP_019405534.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PszF2a_RS20125 (PszF2a_39430) pilT 4279201..4280235 (-) 1035 WP_221292731.1 type IV pilus twitching motility protein PilT Machinery gene
  PszF2a_RS20130 (PszF2a_39440) - 4280302..4280991 (+) 690 WP_011914971.1 YggS family pyridoxal phosphate-dependent enzyme -
  PszF2a_RS20135 (PszF2a_39450) proC 4281002..4281826 (+) 825 WP_221292732.1 pyrroline-5-carboxylate reductase -
  PszF2a_RS20140 (PszF2a_39460) - 4281836..4282426 (+) 591 WP_014598006.1 YggT family protein -
  PszF2a_RS20145 (PszF2a_39470) - 4282696..4284663 (+) 1968 WP_221292733.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38061.83 Da        Isoelectric Point: 6.8359

>NTDB_id=87708 PszF2a_RS20125 WP_221292731.1 4279201..4280235(-) (pilT) [Stutzerimonas stutzeri strain F2a]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDYEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLNMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKTGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDMCL
KGLLAKGLISRDSAREKAKQPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=87708 PszF2a_RS20125 WP_221292731.1 4279201..4280235(-) (pilT) [Stutzerimonas stutzeri strain F2a]
ATGGATATCACTGAACTGCTGGCCTTCAGCGCCAAGCAGGGTGCGTCGGATTTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATCCGCGTCGATGGTGACGTGCGGCGCATCAATCTGCCGGCCATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGATTACGAGGAATTCCTCGAAACCGACTTCTCGTTCGAGGTGCCGGGTGTGGCGCGC
TTCCGCGTCAACGCCTTCAACCAGAACCGCGGCGCCGGCGCCGTGTTCCGGACCATTCCCTCCAAGGTGCTGACCATGGA
GGACCTCAACATGGGTGAGGTCTTCCGCAAGATCACCGACGTGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGCT
CGGGTAAGTCGACCACCCTGGCGGCGATGCTCGATTACCTGAACAACACCAAATACCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTTCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAGGTGCACCGCGACACCCTGGGCTTCTCCGA
GGCGCTGCGTTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTGGGCGAGATGCGCGACCTGGAAACCATTCGCCTGG
CACTGACCGCCGCGGAAACCGGCCACCTCGTGTTCGGCACGCTGCACACCACCTCCGCCGCCAAGACCATCGACCGCGTG
GTCGACGTGTTCCCGGCCGAAGAAAAGTCCATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCGGTGATCTCGCAGAC
CCTGCTGAAGAAGACCGGTGGCGGCCGCGTCGCGGCCCACGAGATCATGATCGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGCGGTTCGCTCGGCATGCAGACCCTGGACATGTGCCTC
AAGGGGCTGCTGGCCAAGGGCCTGATCAGCCGCGACAGCGCGCGGGAAAAGGCCAAGCAGCCCGAGAATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

97.965

100

0.98
  pilT Pseudomonas aeruginosa PAK

94.767

100

0.948
  pilT Acinetobacter baumannii D1279779

84.884

100

0.849
  pilT Acinetobacter baumannii strain A118

84.884

100

0.849
  pilT Acinetobacter nosocomialis M2

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

83.721

100

0.837
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

39.017

100

0.392

Multiple sequence alignment