Detailed information NTG0087707

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PszF2a_RS20120
• Genome accession: NZ_AP024722
• Coordinates: 4277996..4279141 (-)
• Length: 381 a.a.
• NCBI ID: WP_019405534.1
• Uniprot ID: -
• Organism: Stutzerimonas stutzeri strain F2a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4272996..4284141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PszF2a_RS20095 (PszF2a_39370)	-	4273073..4274089 (+)	1017	WP_011914964.1	aspartate carbamoyltransferase catalytic subunit	-
PszF2a_RS20100 (PszF2a_39380)	-	4274089..4275363 (+)	1275	WP_256445384.1	dihydroorotase	-
PszF2a_RS20105 (PszF2a_39390)	-	4275421..4275816 (-)	396	WP_011914966.1	NINE protein	-
PszF2a_RS20110 (PszF2a_39400)	-	4275952..4277166 (+)	1215	WP_221292729.1	putative DNA modification/repair radical SAM protein	-
PszF2a_RS20115 (PszF2a_39410)	-	4277166..4277999 (+)	834	WP_221292730.1	TIGR03915 family putative DNA repair protein	-
PszF2a_RS20120 (PszF2a_39420)	pilU	4277996..4279141 (-)	1146	WP_019405534.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PszF2a_RS20125 (PszF2a_39430)	pilT	4279201..4280235 (-)	1035	WP_221292731.1	type IV pilus twitching motility protein PilT	Machinery gene
PszF2a_RS20130 (PszF2a_39440)	-	4280302..4280991 (+)	690	WP_011914971.1	YggS family pyridoxal phosphate-dependent enzyme	-
PszF2a_RS20135 (PszF2a_39450)	proC	4281002..4281826 (+)	825	WP_221292732.1	pyrroline-5-carboxylate reductase	-
PszF2a_RS20140 (PszF2a_39460)	-	4281836..4282426 (+)	591	WP_014598006.1	YggT family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42566.88 Da
• Isoelectric Point: 6.8858

>NTDB_id=87707 PszF2a_RS20120 WP_019405534.1 4277996..4279141(-) (pilU) [Stutzerimonas stutzeri strain F2a]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPVSKTPMSPEMTRETVHGVMNEQQRREFTENHECNFAISARGIGR
FRVSAFYQRNLAGMVLRRIETNIPTIEELKLPDVLKKLSMTKRGLVLFVGATGTGKSTSLASMIGYRNKNSSGHIISIED
PIEFIHQHQNCIVTQREVGIDTSSFEVALKNTLRQAPDVILIGEIRTRETMDYAVAFAETGHLCLATLHANNANQALDRI
INFFPPDRHNQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLINTPLAADLIRKGEVHELKSLMKRSTELGMQTFDQA
LYNLYVQGEITYEDALLHADSANDLRLLIKLGSETDGEHLTSVSQGLSLEVSDEDPGRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=87707 PszF2a_RS20120 WP_019405534.1 4277996..4279141(-) (pilU) [Stutzerimonas stutzeri strain F2a]
ATGGAATTCGAGAAACTTCTGCGCCTGATGGTCGAAAAAGGCGGCTCCGATCTGTTCATCACGGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGGTGAGCAAGACGCCCATGTCGCCGGAGATGACCCGGGAAACCGTGCACGGCG
TGATGAACGAGCAGCAGCGCCGCGAGTTCACCGAGAACCACGAATGCAACTTCGCCATCAGTGCCCGCGGCATCGGTCGC
TTCCGTGTCAGCGCGTTCTATCAGCGAAACCTGGCCGGCATGGTGCTGCGTCGCATCGAGACCAACATCCCGACCATCGA
AGAGCTCAAGCTGCCGGACGTCCTGAAGAAGCTGTCGATGACCAAACGTGGTCTGGTGCTGTTCGTCGGTGCCACCGGTA
CCGGCAAGTCGACATCGCTGGCCTCGATGATCGGCTACCGCAACAAGAACTCCAGCGGGCACATCATCTCCATTGAAGAC
CCGATCGAATTCATCCACCAGCACCAGAATTGCATCGTCACCCAGCGCGAAGTGGGCATCGACACCAGCTCCTTCGAGGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCTGACGTGATCCTCATCGGCGAGATCCGTACCCGCGAAACCATGGATTACG
CGGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGCCGGACCGGCACAACCAGGTCTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTTCCGACCCCGGACGGCAAGGGTCGTCGTGCGGTGATCGAGGTGCTGATCAATACGCCGCTGGCGGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAATCGCTGATGAAGCGCTCCACCGAGCTGGGTATGCAGACCTTCGACCAGGCG
CTGTACAACCTCTACGTGCAGGGGGAGATCACCTACGAAGATGCCCTGCTGCATGCTGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGGGTTCGGAAACCGACGGTGAGCACCTGACCAGCGTATCCCAGGGCCTGAGCCTGGAGGTCAGCGACG
AGGACCCGGGCCGGCGCTTCCGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	95.013	100	0.95
pilU	Acinetobacter baylyi ADP1	66.106	93.701	0.619
pilU	Vibrio cholerae strain A1552	59.429	91.864	0.546
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.953	88.976	0.391