Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM61_RS09375 Genome accession   NZ_CP133779
Coordinates   2057470..2058513 (-) Length   347 a.a.
NCBI ID   WP_322250849.1    Uniprot ID   A0AAU0SDD6
Organism   Undibacterium sp. CCC3.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2052470..2063513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM61_RS09345 (RHM61_09345) glcE 2052475..2053539 (+) 1065 WP_322250842.1 glycolate oxidase subunit GlcE -
  RHM61_RS09350 (RHM61_09350) glcF 2053546..2054769 (+) 1224 WP_322250843.1 glycolate oxidase subunit GlcF -
  RHM61_RS09355 (RHM61_09355) - 2054766..2055098 (+) 333 WP_322250844.1 CidA/LrgA family protein -
  RHM61_RS09360 (RHM61_09360) - 2055095..2055820 (+) 726 WP_322250845.1 LrgB family protein -
  RHM61_RS09365 (RHM61_09365) - 2055823..2056308 (-) 486 WP_322250847.1 glutathione peroxidase -
  RHM61_RS09370 (RHM61_09370) pilU 2056311..2057447 (-) 1137 WP_322250848.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHM61_RS09375 (RHM61_09375) pilT 2057470..2058513 (-) 1044 WP_322250849.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM61_RS09380 (RHM61_09380) - 2058609..2059343 (+) 735 WP_322250850.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM61_RS09385 (RHM61_09385) - 2059913..2060068 (+) 156 WP_322251080.1 RRXRR domain-containing protein -
  RHM61_RS09390 (RHM61_09390) proC 2060286..2061113 (+) 828 WP_322250851.1 pyrroline-5-carboxylate reductase -
  RHM61_RS09395 (RHM61_09395) - 2061304..2063079 (+) 1776 WP_322250852.1 glycosyltransferase family 39 protein -
  RHM61_RS09400 (RHM61_09400) - 2063085..2063417 (-) 333 WP_322250853.1 YqjK family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38105.66 Da        Isoelectric Point: 6.5769

>NTDB_id=876700 RHM61_RS09375 WP_322250849.1 2057470..2058513(-) (pilT) [Undibacterium sp. CCC3.4]
MDISELLAFSVKNNASDLHLSSGLPPMIRVHGDVRRINLPPLEHKDVHELIYDIMNDSQRKAYEETLECDFSFSIPGLAR
FRVNAFNQERGAAAVMRTIPSKILSLEQLNAPRIFGDLALKPRGLVLVTGPTGSGKSTTLAAMVNHLNENEYGHILTVED
PIEFVHESKKCLINQREVGPHTMSFNNALRSALREDPDAILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
VDVFPAEEKEMVRSMLSESLQAVISQTLLKTKDGSGRVAAHEIMLGSPAIRNLIRESKIAQMYSAIQTGSNLGMQTLDQN
LTDLVRRNVISVATARAAAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=876700 RHM61_RS09375 WP_322250849.1 2057470..2058513(-) (pilT) [Undibacterium sp. CCC3.4]
ATGGACATTTCTGAACTGCTGGCATTCTCTGTCAAGAATAATGCCTCCGATCTGCACTTATCTTCCGGCTTACCGCCGAT
GATACGGGTACATGGCGATGTGCGACGGATTAATTTGCCGCCGCTGGAACATAAAGATGTGCATGAACTCATTTATGACA
TCATGAATGATTCCCAGCGTAAGGCTTACGAAGAAACGCTGGAATGCGATTTCTCGTTTTCCATTCCTGGACTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGGAACGTGGTGCGGCGGCCGTCATGCGCACGATTCCATCCAAAATTCTCTCACTTGA
GCAACTCAATGCCCCGCGTATTTTCGGCGATCTCGCGCTCAAACCGCGCGGTTTGGTTTTGGTGACAGGCCCTACCGGCT
CGGGCAAATCAACCACGCTCGCGGCCATGGTCAATCACCTCAATGAAAATGAATACGGCCACATTCTGACGGTAGAAGAT
CCTATCGAATTCGTGCACGAATCGAAAAAATGTCTGATCAACCAGCGCGAAGTCGGGCCACACACGATGTCGTTCAATAA
TGCGCTGCGTTCGGCCTTGCGCGAAGATCCCGATGCCATTCTGGTCGGCGAGTTGCGCGATCTCGAAACCATACGCTTGG
CATTGACGGCGGCCGAAACCGGCCATCTGGTGTTCGGCACCCTGCATACTTCGTCTGCCGCCAAAACCATCGACCGTATC
GTCGACGTGTTTCCGGCCGAAGAAAAAGAAATGGTCCGTTCTATGCTGTCAGAGTCGCTGCAAGCGGTGATTTCACAAAC
TCTGCTCAAAACCAAAGACGGCTCCGGTCGCGTCGCCGCCCATGAAATCATGCTTGGCTCACCGGCGATTCGGAATTTGA
TACGCGAATCAAAAATCGCTCAGATGTACTCAGCCATCCAAACCGGCAGTAATCTGGGCATGCAAACGCTGGACCAGAAT
CTGACCGATCTGGTACGACGCAATGTGATTTCTGTCGCCACGGCACGCGCCGCGGCGAAAATCCCGGACAACTTCCCCGG
CTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

77.971

99.424

0.775
  pilT Acinetobacter baumannii strain A118

77.971

99.424

0.775
  pilT Acinetobacter nosocomialis M2

77.971

99.424

0.775
  pilT Pseudomonas aeruginosa PAK

76.812

99.424

0.764
  pilT Pseudomonas stutzeri DSM 10701

75.652

99.424

0.752
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

75.516

97.695

0.738
  pilT Legionella pneumophila strain ERS1305867

75.516

97.695

0.738
  pilT Neisseria meningitidis 8013

69.706

97.983

0.683
  pilT Neisseria gonorrhoeae MS11

69.412

97.983

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.606

95.101

0.671
  pilT Vibrio cholerae strain A1552

70.606

95.101

0.671
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Acinetobacter baylyi ADP1

41.908

99.712

0.418
  pilU Vibrio cholerae strain A1552

42.9

95.389

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.493

96.542

0.401