Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RHM61_RS09370 Genome accession   NZ_CP133779
Coordinates   2056311..2057447 (-) Length   378 a.a.
NCBI ID   WP_322250848.1    Uniprot ID   A0AAU0SDN0
Organism   Undibacterium sp. CCC3.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2051311..2062447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM61_RS09345 (RHM61_09345) glcE 2052475..2053539 (+) 1065 WP_322250842.1 glycolate oxidase subunit GlcE -
  RHM61_RS09350 (RHM61_09350) glcF 2053546..2054769 (+) 1224 WP_322250843.1 glycolate oxidase subunit GlcF -
  RHM61_RS09355 (RHM61_09355) - 2054766..2055098 (+) 333 WP_322250844.1 CidA/LrgA family protein -
  RHM61_RS09360 (RHM61_09360) - 2055095..2055820 (+) 726 WP_322250845.1 LrgB family protein -
  RHM61_RS09365 (RHM61_09365) - 2055823..2056308 (-) 486 WP_322250847.1 glutathione peroxidase -
  RHM61_RS09370 (RHM61_09370) pilU 2056311..2057447 (-) 1137 WP_322250848.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHM61_RS09375 (RHM61_09375) pilT 2057470..2058513 (-) 1044 WP_322250849.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM61_RS09380 (RHM61_09380) - 2058609..2059343 (+) 735 WP_322250850.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM61_RS09385 (RHM61_09385) - 2059913..2060068 (+) 156 WP_322251080.1 RRXRR domain-containing protein -
  RHM61_RS09390 (RHM61_09390) proC 2060286..2061113 (+) 828 WP_322250851.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41854.17 Da        Isoelectric Point: 6.8326

>NTDB_id=876699 RHM61_RS09370 WP_322250848.1 2056311..2057447(-) (pilU) [Undibacterium sp. CCC3.4]
MERDQATKFMNDLLRLMLSKNGSDLFITAEFPPAFKIDGRVTPVSNQPLTAAHTVDLARAIMNDKQASEFESSKECNFAI
NPAGMGRFRVSAFMQQGRVGLVLRTITTAIPKLADLGLPEQLKEVSLTKRGLVIMVGATGSGKSTTLAAMLGYRNENSYG
HIITIEDPIEYVHPHGNCIVTQREVGIDTADWGSALKNSLRQAPDVIQIGEIRDRETMDFAVAFAETGHLCLATLHANSS
NQALDRIINFFPEERRAQLLMDLSLNLKAVISQRLIPVKGRKGRIAAVEVLLNTPLISDMIFKGQVHEMKEIMKKSRELG
MQTFDQALFDLHEADKITYEDALRNADSVNELRLAIMLQGKDSKDRDLSAGTHHLGIL

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=876699 RHM61_RS09370 WP_322250848.1 2056311..2057447(-) (pilU) [Undibacterium sp. CCC3.4]
ATGGAACGCGATCAGGCTACCAAATTCATGAATGACTTGCTCAGACTGATGCTGAGCAAAAACGGATCAGATTTATTCAT
CACCGCCGAATTCCCGCCGGCTTTCAAGATCGATGGCCGCGTCACACCGGTATCGAATCAGCCGCTGACGGCCGCGCACA
CGGTCGATTTGGCACGCGCGATCATGAATGACAAGCAAGCCTCGGAATTCGAATCGAGCAAGGAATGTAATTTTGCGATC
AACCCGGCCGGGATGGGACGCTTTCGGGTGTCTGCCTTCATGCAACAGGGCCGGGTCGGTTTGGTCTTGCGCACCATCAC
CACCGCCATTCCCAAGTTAGCCGATCTCGGCTTACCGGAACAATTGAAAGAAGTCTCGCTGACCAAGCGCGGCTTGGTGA
TCATGGTCGGTGCCACCGGCTCCGGCAAATCGACCACGCTGGCGGCCATGCTTGGCTATCGCAATGAAAACAGCTATGGC
CACATCATCACGATTGAAGATCCTATCGAATATGTGCATCCACACGGCAATTGCATCGTCACGCAGCGCGAAGTCGGCAT
CGACACCGCAGACTGGGGCTCGGCACTGAAAAATTCTCTGCGACAAGCGCCCGACGTGATACAGATTGGTGAAATTCGCG
ACCGCGAAACCATGGATTTTGCCGTCGCCTTTGCCGAAACCGGGCACTTGTGTTTAGCCACCCTACATGCCAACAGCTCG
AATCAAGCGCTCGACCGTATCATCAACTTTTTCCCGGAAGAACGCCGCGCACAATTGCTGATGGACTTGTCGCTCAACCT
CAAGGCCGTGATTTCGCAACGCTTGATTCCGGTCAAAGGCCGCAAAGGCCGCATCGCTGCGGTCGAAGTACTGCTCAATA
CGCCTTTAATTTCTGACATGATTTTCAAAGGCCAAGTTCATGAAATGAAAGAAATCATGAAGAAGTCGCGCGAACTCGGT
ATGCAAACCTTCGATCAAGCTTTGTTCGATTTGCATGAAGCCGACAAAATCACTTATGAAGATGCCCTGCGCAACGCCGA
TTCCGTCAATGAACTGCGCTTGGCCATCATGTTGCAAGGTAAAGACTCCAAAGACCGTGACCTCAGCGCCGGCACTCACC
ATTTAGGGATACTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(99-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.162

91.534

0.587
  pilU Acinetobacter baylyi ADP1

58.496

94.974

0.556
  pilU Vibrio cholerae strain A1552

55.056

94.18

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.228

91.799

0.397
  pilT Pseudomonas aeruginosa PAK

43.323

89.153

0.386
  pilT Legionella pneumophila strain Lp02

41.39

87.566

0.362
  pilT Legionella pneumophila strain ERS1305867

41.39

87.566

0.362
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.492

83.333

0.362
  pilT Vibrio cholerae strain A1552

43.492

83.333

0.362