Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RHM62_RS05250 Genome accession   NZ_CP133777
Coordinates   1126323..1127366 (-) Length   347 a.a.
NCBI ID   WP_322124501.1    Uniprot ID   -
Organism   Actimicrobium sp. CCC2.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1121323..1132366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHM62_RS05240 (RHM62_05240) - 1122330..1124828 (+) 2499 WP_322124499.1 adenosylcobalamin-dependent ribonucleoside-diphosphate reductase -
  RHM62_RS05245 (RHM62_05245) pilU 1125136..1126272 (-) 1137 WP_322124500.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RHM62_RS05250 (RHM62_05250) pilT 1126323..1127366 (-) 1044 WP_322124501.1 type IV pilus twitching motility protein PilT Machinery gene
  RHM62_RS05255 (RHM62_05255) - 1127485..1128189 (+) 705 WP_322124502.1 YggS family pyridoxal phosphate-dependent enzyme -
  RHM62_RS05260 (RHM62_05260) proC 1128179..1129018 (+) 840 WP_322124503.1 pyrroline-5-carboxylate reductase -
  RHM62_RS05265 (RHM62_05265) ubiA 1129031..1129885 (-) 855 WP_322125328.1 4-hydroxybenzoate octaprenyltransferase -
  RHM62_RS05270 (RHM62_05270) - 1129905..1130852 (-) 948 WP_322124504.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38114.81 Da        Isoelectric Point: 6.9821

>NTDB_id=876650 RHM62_RS05250 WP_322124501.1 1126323..1127366(-) (pilT) [Actimicrobium sp. CCC2.4]
MDISELLAFSVKNNASDLHLSAGLPPMIRVHGDVRRINLPPLEHKDVHAMIYDIMNDGQRKAYEEKLEVDFSFAIPGLAR
FRVNAFNQDRGAAAVLRTIPSKILSLEQLNTPRIFAELSLKPRGLVLVTGPTGSGKSTTLAAMVNHVNENEYAHILTIED
PIEFVHESKKCLINQREVGPHTHSFSAALRSALREDPDVVLVGELRDLETIRLALSAAETGHLVFGTLHTSSAAKTIDRI
VDVFPGDEKEMVRAMLSESLQAVISQTLLKTKDGAGRVAAHEIMLGTPAIRNLIRESKIAQMYSAIQTGMNMGMQTLDHN
LTDLVRRNVISPQVARSAAKTPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=876650 RHM62_RS05250 WP_322124501.1 1126323..1127366(-) (pilT) [Actimicrobium sp. CCC2.4]
ATGGACATCTCCGAACTGCTCGCGTTCTCCGTCAAGAACAACGCCTCCGACCTGCATCTGTCCGCTGGCTTGCCACCGAT
GATACGGGTCCATGGTGATGTCCGTCGCATCAATCTGCCGCCGCTGGAGCACAAGGATGTGCACGCGATGATCTACGACA
TCATGAACGATGGCCAGCGCAAGGCCTACGAAGAAAAGCTCGAAGTCGACTTCTCGTTTGCCATTCCCGGACTGGCGCGC
TTTCGCGTCAATGCCTTCAACCAGGACCGTGGTGCCGCCGCCGTGTTGCGCACTATTCCGTCCAAGATCCTCAGCCTCGA
ACAACTCAATACGCCGCGCATCTTTGCCGAACTGTCGCTCAAGCCGCGCGGCCTGGTGCTGGTGACCGGTCCGACCGGCT
CCGGCAAATCAACCACGCTCGCGGCAATGGTGAACCATGTCAATGAAAACGAATACGCCCACATCCTGACGATCGAAGAC
CCGATCGAATTCGTTCACGAATCCAAAAAATGCCTGATCAACCAGCGCGAAGTCGGCCCGCACACGCATAGCTTCAGCGC
GGCCTTGCGCTCGGCTTTGCGCGAAGATCCGGACGTGGTGCTGGTCGGCGAATTACGTGACCTCGAAACCATCCGGCTGG
CGTTGTCGGCGGCAGAAACCGGCCATCTGGTGTTCGGCACGCTGCACACGTCGTCGGCGGCCAAGACCATCGACCGTATC
GTCGATGTCTTCCCCGGCGATGAAAAGGAAATGGTGCGGGCGATGCTGTCAGAATCGTTGCAGGCAGTGATTTCGCAGAC
GCTGCTGAAAACCAAGGATGGTGCCGGTCGCGTGGCCGCACATGAAATCATGCTGGGCACGCCGGCCATCCGCAACCTGA
TCCGCGAGTCGAAGATCGCGCAGATGTATTCGGCGATCCAGACCGGCATGAACATGGGCATGCAAACGCTGGATCACAAC
CTGACCGATCTGGTACGTCGCAATGTCATTTCGCCGCAAGTCGCCCGCTCTGCCGCCAAGACACCGGAAAATTTCCCCGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.101

99.424

0.767
  pilT Acinetobacter baumannii D1279779

77.101

99.424

0.767
  pilT Acinetobacter baumannii strain A118

77.101

99.424

0.767
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Pseudomonas stutzeri DSM 10701

74.493

99.424

0.741
  pilT Acinetobacter baylyi ADP1

72.754

99.424

0.723
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Vibrio cholerae strain A1552

70.03

97.118

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.118

0.68
  pilT Neisseria meningitidis 8013

68.406

99.424

0.68
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Acinetobacter baylyi ADP1

40.751

99.712

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.597

96.542

0.392
  pilU Vibrio cholerae strain A1552

39.42

99.424

0.392