Detailed information NTG0876649

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RHM62_RS05245
• Genome accession: NZ_CP133777
• Coordinates: 1125136..1126272 (-)
• Length: 378 a.a.
• NCBI ID: WP_322124500.1
• Uniprot ID: -
• Organism: Actimicrobium sp. CCC2.4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1120136..1131272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHM62_RS05235 (RHM62_05235)	-	1121226..1122107 (+)	882	WP_322124498.1	LysR substrate-binding domain-containing protein	-
RHM62_RS05240 (RHM62_05240)	-	1122330..1124828 (+)	2499	WP_322124499.1	adenosylcobalamin-dependent ribonucleoside-diphosphate reductase	-
RHM62_RS05245 (RHM62_05245)	pilU	1125136..1126272 (-)	1137	WP_322124500.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RHM62_RS05250 (RHM62_05250)	pilT	1126323..1127366 (-)	1044	WP_322124501.1	type IV pilus twitching motility protein PilT	Machinery gene
RHM62_RS05255 (RHM62_05255)	-	1127485..1128189 (+)	705	WP_322124502.1	YggS family pyridoxal phosphate-dependent enzyme	-
RHM62_RS05260 (RHM62_05260)	proC	1128179..1129018 (+)	840	WP_322124503.1	pyrroline-5-carboxylate reductase	-
RHM62_RS05265 (RHM62_05265)	ubiA	1129031..1129885 (-)	855	WP_322125328.1	4-hydroxybenzoate octaprenyltransferase	-
RHM62_RS05270 (RHM62_05270)	-	1129905..1130852 (-)	948	WP_322124504.1	LysR substrate-binding domain-containing protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42090.59 Da
• Isoelectric Point: 7.0607

>NTDB_id=876649 RHM62_RS05245 WP_322124500.1 1125136..1126272(-) (pilU) [Actimicrobium sp. CCC2.4]
MEREQATKFMNDLLRLMVSKNGSDLFITTDFAPAFKIDGKIMPVSNQLLTASHTIELARAIMSDRQAAEFEATKECNFAI
SPGGLGRFRVSAFIQQQKVGMVLRTITTEIPTVEQLGLPPQLKDIAMTKRGLVIMVGATGSGKSTTLAAMIGYRNEHSHG
HIITIEDPVEYIHPHRNCIVTQRDVGLDTDSWATALKNALRQAPDVIQIGEIRDRETMDHAIAFAETGHLCLATLHANSA
NQALDRIINFFPEERRQQLLMDLSLNLKAMVSQRLVPLRDTKGRCVAVEIMLNSPLISDLIFKGDVHEIKEIMKKSRELG
MVTFDQALFDLYEADKISYENALRNADSVNDLRLAIKLRGKDAKGRDLSKGTEHLGIV

Nucleotide

• Length: 1137 bp

>NTDB_id=876649 RHM62_RS05245 WP_322124500.1 1125136..1126272(-) (pilU) [Actimicrobium sp. CCC2.4]
ATGGAACGCGAACAAGCCACCAAATTCATGAACGACCTGCTGCGCCTGATGGTCAGCAAGAACGGTTCCGACCTGTTCAT
CACGACCGACTTTGCACCGGCCTTCAAGATCGACGGCAAGATCATGCCGGTGTCGAACCAGTTGCTGACTGCGAGCCACA
CGATCGAACTGGCGCGCGCCATCATGAGCGACCGGCAAGCCGCCGAATTCGAAGCCACCAAGGAATGTAATTTCGCGATC
AGCCCGGGCGGACTGGGACGCTTCCGCGTCTCGGCTTTCATCCAGCAGCAAAAGGTCGGCATGGTCCTGCGCACCATCAC
CACCGAAATCCCGACCGTCGAACAGCTCGGGCTGCCGCCGCAACTCAAGGACATCGCGATGACCAAGCGCGGCCTGGTCA
TCATGGTCGGCGCCACCGGCTCCGGCAAATCGACCACGCTGGCAGCGATGATCGGCTACCGCAACGAGCACAGCCACGGT
CACATCATCACGATCGAAGATCCGGTCGAATACATCCACCCGCACCGCAACTGCATCGTCACGCAGCGCGATGTCGGTCT
CGATACCGACAGCTGGGCCACGGCGCTCAAGAACGCGCTGCGCCAGGCACCGGACGTCATCCAGATCGGTGAAATCCGCG
ACCGCGAAACCATGGACCACGCGATTGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AATCAGGCGCTTGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCCAGCAATTGCTGATGGACTTGTCACTGAACCT
GAAAGCCATGGTCTCGCAACGGCTGGTGCCACTGCGCGATACCAAAGGACGCTGCGTGGCGGTCGAAATCATGCTGAATT
CACCGCTGATTTCGGACCTGATCTTCAAGGGCGACGTGCACGAGATCAAAGAAATCATGAAAAAGTCCCGCGAACTGGGC
ATGGTGACCTTCGACCAGGCGCTGTTCGACCTGTACGAAGCCGACAAGATCTCGTATGAAAACGCACTGCGCAACGCCGA
TTCGGTCAACGACCTGCGCCTAGCGATCAAGCTGCGCGGCAAGGATGCGAAGGGACGCGATTTGTCCAAGGGGACGGAGC
ATCTGGGGATTGTTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (87-276)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	63.361	96.032	0.608
pilU	Acinetobacter baylyi ADP1	58.496	94.974	0.556
pilU	Vibrio cholerae strain A1552	54.213	94.18	0.511
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.214	89.153	0.394
pilT	Legionella pneumophila strain Lp02	41.39	87.566	0.362
pilT	Legionella pneumophila strain ERS1305867	41.39	87.566	0.362