Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RG903_RS11815 Genome accession   NZ_CP133759
Coordinates   2363648..2364778 (+) Length   376 a.a.
NCBI ID   WP_028989668.1    Uniprot ID   -
Organism   Thermithiobacillus tepidarius DSM 3134     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2358648..2369778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG903_RS11785 (RG903_11760) - 2359209..2359766 (-) 558 WP_028989674.1 porin family protein -
  RG903_RS11790 (RG903_11765) - 2360081..2360398 (-) 318 WP_028989673.1 DUF167 domain-containing protein -
  RG903_RS11795 (RG903_11770) - 2360395..2360691 (-) 297 WP_028989672.1 YggT family protein -
  RG903_RS11800 (RG903_11775) proC 2360698..2361585 (-) 888 WP_081662663.1 pyrroline-5-carboxylate reductase -
  RG903_RS11805 (RG903_11780) - 2361658..2362389 (-) 732 WP_028989670.1 YggS family pyridoxal phosphate-dependent enzyme -
  RG903_RS11810 (RG903_11785) pilT 2362568..2363623 (+) 1056 WP_028989669.1 type IV pilus twitching motility protein PilT Machinery gene
  RG903_RS11815 (RG903_11790) pilU 2363648..2364778 (+) 1131 WP_028989668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RG903_RS11825 (RG903_11800) - 2365109..2367373 (-) 2265 WP_028989667.1 SBBP repeat-containing protein -
  RG903_RS11830 (RG903_11805) - 2367575..2369278 (+) 1704 WP_028989666.1 GspE/PulE family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41804.07 Da        Isoelectric Point: 6.1214

>NTDB_id=876406 RG903_RS11815 WP_028989668.1 2363648..2364778(+) (pilU) [Thermithiobacillus tepidarius DSM 3134]
MSFRDLLVLMNQRNASDLYLTVGAPPTFKVDGKSVPVGSAPLKPMETVALAREMMSEEQLNEFESLKELNMALSVPQVGR
FRVNAFYQRNEVGMVLRAIKTEIPDLEALRLPPILKDLAMTPRGLVLFVGATGSGKSTSLAAMIGYRNRNAGGHILTIED
PIEFIHKHEKSIVNQREVGIDTKSYEEALKNALRQAPDVILIGEIRSRETMDHAVAFAETGHLCLSTLHANNANQALDRI
INFFPEDRRHQLLMDLSLNLKAVVSQRLVPRKSGQGRRAAIEILINTPTVADLIYKGEVGSIKEVMAKSTELGMQTFDQS
LLQLYMEDEISYEDALHNADSMNDLRLAIKLECMRQGRPEPRNGDAAQSGGLRVEE

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=876406 RG903_RS11815 WP_028989668.1 2363648..2364778(+) (pilU) [Thermithiobacillus tepidarius DSM 3134]
GTGAGTTTTAGAGATCTGCTCGTGCTGATGAATCAGCGCAACGCCTCCGACTTGTACCTGACCGTCGGCGCGCCGCCGAC
TTTCAAGGTGGACGGCAAGAGCGTGCCGGTCGGCAGCGCACCGCTCAAGCCCATGGAAACGGTGGCGCTGGCCCGCGAAA
TGATGAGCGAGGAGCAACTGAACGAATTCGAGAGCCTGAAGGAGCTGAACATGGCCCTGAGCGTGCCCCAGGTGGGCCGC
TTCCGCGTCAATGCCTTCTATCAGCGCAACGAAGTGGGCATGGTGCTGCGCGCCATCAAGACCGAAATCCCGGATCTCGA
AGCCCTGCGCCTGCCGCCCATCCTCAAGGATCTGGCCATGACCCCGCGCGGCCTGGTGCTCTTCGTCGGCGCGACGGGCT
CCGGCAAGTCCACCTCGCTGGCGGCCATGATCGGCTATCGCAACCGCAACGCCGGCGGGCACATCCTCACCATCGAGGAC
CCCATCGAATTCATTCACAAGCACGAGAAGTCCATCGTTAATCAGCGCGAGGTGGGCATCGACACCAAGTCCTACGAGGA
GGCCCTGAAAAACGCCCTGCGCCAAGCCCCGGACGTGATCCTCATCGGCGAAATCCGCTCGCGCGAGACCATGGATCACG
CCGTCGCCTTCGCCGAAACCGGCCACCTGTGCCTGTCCACCCTGCACGCCAACAACGCCAACCAGGCCCTGGATCGCATC
ATCAACTTCTTCCCCGAGGACCGGCGCCACCAACTGCTCATGGACCTGTCCCTGAACCTGAAAGCGGTGGTATCCCAGCG
GCTGGTCCCCAGGAAGTCGGGCCAGGGCCGGCGGGCCGCCATCGAGATCCTCATCAACACCCCCACCGTGGCCGACCTGA
TCTACAAGGGCGAGGTGGGCAGCATCAAGGAAGTGATGGCCAAGTCCACCGAACTGGGCATGCAGACCTTCGACCAATCC
CTGCTGCAGCTCTACATGGAAGACGAGATCAGCTACGAGGACGCCCTGCACAACGCCGACTCCATGAACGACCTGCGCCT
GGCGATCAAGCTGGAATGCATGCGCCAGGGCCGCCCGGAACCCAGGAACGGCGATGCGGCCCAGAGCGGCGGGCTGCGGG
TGGAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(30-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.952

93.351

0.625
  pilU Acinetobacter baylyi ADP1

59.659

93.617

0.559
  pilU Vibrio cholerae strain A1552

56.319

96.809

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.463

89.096

0.423
  pilT Pseudomonas aeruginosa PAK

43.068

90.16

0.388
  pilT Acinetobacter baumannii strain A118

42.687

89.096

0.38
  pilT Acinetobacter baumannii D1279779

42.687

89.096

0.38
  pilT Acinetobacter nosocomialis M2

42.687

89.096

0.38
  pilT Pseudomonas stutzeri DSM 10701

42.183

90.16

0.38
  pilT Neisseria meningitidis 8013

41.329

92.021

0.38
  pilT Neisseria gonorrhoeae MS11

41.329

92.021

0.38
  pilT Acinetobacter baylyi ADP1

43.827

86.17

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.827

86.17

0.378
  pilT Vibrio cholerae strain A1552

43.827

86.17

0.378
  pilT Legionella pneumophila strain Lp02

41.791

89.096

0.372
  pilT Legionella pneumophila strain ERS1305867

41.791

89.096

0.372