Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RG903_RS11810 Genome accession   NZ_CP133759
Coordinates   2362568..2363623 (+) Length   351 a.a.
NCBI ID   WP_028989669.1    Uniprot ID   -
Organism   Thermithiobacillus tepidarius DSM 3134     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2357568..2368623
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RG903_RS11775 (RG903_11750) apaG 2358178..2358555 (+) 378 WP_028989676.1 Co2+/Mg2+ efflux protein ApaG -
  RG903_RS11780 (RG903_11755) - 2358559..2359158 (+) 600 WP_028989675.1 class I SAM-dependent methyltransferase -
  RG903_RS11785 (RG903_11760) - 2359209..2359766 (-) 558 WP_028989674.1 porin family protein -
  RG903_RS11790 (RG903_11765) - 2360081..2360398 (-) 318 WP_028989673.1 DUF167 domain-containing protein -
  RG903_RS11795 (RG903_11770) - 2360395..2360691 (-) 297 WP_028989672.1 YggT family protein -
  RG903_RS11800 (RG903_11775) proC 2360698..2361585 (-) 888 WP_081662663.1 pyrroline-5-carboxylate reductase -
  RG903_RS11805 (RG903_11780) - 2361658..2362389 (-) 732 WP_028989670.1 YggS family pyridoxal phosphate-dependent enzyme -
  RG903_RS11810 (RG903_11785) pilT 2362568..2363623 (+) 1056 WP_028989669.1 type IV pilus twitching motility protein PilT Machinery gene
  RG903_RS11815 (RG903_11790) pilU 2363648..2364778 (+) 1131 WP_028989668.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RG903_RS11825 (RG903_11800) - 2365109..2367373 (-) 2265 WP_028989667.1 SBBP repeat-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38584.53 Da        Isoelectric Point: 6.8398

>NTDB_id=876405 RG903_RS11810 WP_028989669.1 2362568..2363623(+) (pilT) [Thermithiobacillus tepidarius DSM 3134]
MDITELLTFAVKNKASDTHLSAGLPPMVRINGEIKRLNVEPLDAKAVHAMIYDIMGDKQRKVFEENLEIDFSIDLGGVAR
FRVNAFNQDRGPAAAFRTIPSEVLSLEDLNAPPIFKQIIDVPRGLILVTGPTGSGKSTTLAAMINHVNENRHEHILTIED
PIEFVHQSKKCLINQREVGPQTHSFANALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAPKTIDRI
IDVFPAAEKDMVRAMLSESLRAVISQTLLKTADGQGRVAAHEIMIGTPAIRNLIREAKVAQMVSVIQTGQSVGMQTLDQN
LLDLVKARRVRAEDAMRKAQNKDLFANVGAM

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=876405 RG903_RS11810 WP_028989669.1 2362568..2363623(+) (pilT) [Thermithiobacillus tepidarius DSM 3134]
ATGGACATTACGGAACTGCTGACCTTCGCGGTCAAGAACAAAGCGTCGGATACCCACCTGTCGGCGGGCCTGCCGCCCAT
GGTGCGCATCAACGGCGAGATCAAGCGGCTCAATGTGGAGCCGCTGGACGCCAAGGCCGTGCACGCCATGATCTACGACA
TCATGGGCGACAAGCAGCGCAAGGTCTTCGAGGAGAACCTGGAAATCGACTTCTCCATCGATCTCGGCGGCGTCGCGCGC
TTTCGCGTCAACGCCTTCAACCAGGACCGCGGCCCCGCGGCGGCCTTCCGCACCATTCCCTCTGAGGTGCTGAGCCTGGA
GGACCTGAACGCCCCGCCGATCTTCAAGCAGATCATCGACGTGCCGCGCGGCCTGATCCTGGTCACCGGCCCGACCGGCT
CCGGCAAGTCCACCACCCTGGCGGCCATGATCAATCATGTCAACGAGAACCGGCACGAACACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACCAGTCCAAGAAGTGCCTGATCAACCAGCGCGAAGTGGGGCCGCAGACCCACTCCTTCGCCAA
TGCCCTGCGCTCGGCCCTGCGCGAGGACCCGGACATCATCCTGGTGGGCGAAATGCGCGACCTGGAGACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGCCACCTGGTCTTCGGCACCCTGCATACCAGCTCCGCGCCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCGGCCGAAAAGGACATGGTGCGCGCCATGCTGTCCGAGTCCCTGCGGGCGGTGATCTCGCAGAC
CCTGCTCAAGACCGCCGACGGCCAGGGCCGGGTGGCGGCCCACGAGATCATGATCGGCACGCCGGCCATCCGCAATCTCA
TCCGCGAGGCCAAAGTCGCGCAAATGGTCTCGGTCATCCAGACCGGCCAGTCCGTCGGCATGCAGACCCTGGACCAGAAC
CTGCTCGACCTCGTGAAGGCGCGGCGGGTGCGCGCGGAGGATGCCATGCGCAAGGCCCAGAACAAGGACCTCTTCGCCAA
TGTCGGCGCCATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.884

98.291

0.707
  pilT Acinetobacter baumannii D1279779

71.884

98.291

0.707
  pilT Acinetobacter baumannii strain A118

71.884

98.291

0.707
  pilT Pseudomonas aeruginosa PAK

71.014

98.291

0.698
  pilT Pseudomonas stutzeri DSM 10701

70.725

98.291

0.695
  pilT Legionella pneumophila strain Lp02

68.696

98.291

0.675
  pilT Legionella pneumophila strain ERS1305867

68.696

98.291

0.675
  pilT Acinetobacter baylyi ADP1

72.615

92.593

0.672
  pilT Neisseria meningitidis 8013

67.93

97.721

0.664
  pilT Neisseria gonorrhoeae MS11

67.638

97.721

0.661
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.059

96.866

0.65
  pilT Vibrio cholerae strain A1552

67.059

96.866

0.65
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.149

99.145

0.507
  pilU Acinetobacter baylyi ADP1

43.824

96.866

0.425
  pilU Pseudomonas stutzeri DSM 10701

41.108

97.721

0.402
  pilU Vibrio cholerae strain A1552

39.528

96.581

0.382