Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MCIT9_RS05045 Genome accession   NZ_AP024714
Coordinates   951583..952617 (-) Length   344 a.a.
NCBI ID   WP_317706319.1    Uniprot ID   -
Organism   Methylomarinovum caldicuralii strain IT-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 946583..957617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCIT9_RS05015 (MIT9_P0965) - 946715..947281 (+) 567 WP_317706313.1 YqgE/AlgH family protein -
  MCIT9_RS05020 (MIT9_P0966) ruvX 947274..947708 (+) 435 WP_317706314.1 Holliday junction resolvase RuvX -
  MCIT9_RS05025 (MIT9_P0967) pyrR 947705..948217 (+) 513 WP_317706315.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MCIT9_RS05030 (MIT9_P0968) - 948214..949173 (+) 960 WP_317706316.1 aspartate carbamoyltransferase catalytic subunit -
  MCIT9_RS05035 (MIT9_P0969) - 949170..950444 (+) 1275 WP_317706317.1 dihydroorotase -
  MCIT9_RS05040 (MIT9_P0970) pilU 950447..951571 (-) 1125 WP_317706318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MCIT9_RS05045 (MIT9_P0971) pilT 951583..952617 (-) 1035 WP_317706319.1 type IV pilus twitching motility protein PilT Machinery gene
  MCIT9_RS05050 (MIT9_P0972) - 952646..953347 (+) 702 WP_317706320.1 YggS family pyridoxal phosphate-dependent enzyme -
  MCIT9_RS05055 (MIT9_P0973) - 953352..953699 (-) 348 WP_317706321.1 hypothetical protein -
  MCIT9_RS05060 (MIT9_P0974) - 953696..955582 (-) 1887 WP_317706322.1 nucleoside-diphosphate sugar epimerase/dehydratase -
  MCIT9_RS05065 (MIT9_P0975) - 955794..956654 (+) 861 WP_317706323.1 porin family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38255.90 Da        Isoelectric Point: 7.0358

>NTDB_id=87639 MCIT9_RS05045 WP_317706319.1 951583..952617(-) (pilT) [Methylomarinovum caldicuralii strain IT-9]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINVPPLEHKEVHALVYDIMNDKQRRDYEEYLETDFSFELPGVAR
FRVNAFHQDRGAAAVFRTIPSKVLTLEDLGCPPFFREVTQKPRGLILVTGPTGSGKSTTLAAMIDYINSNNYSHILTVED
PIEFVHQSKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAAEKAMIRSMLSESLQAVIAQTLLKKIGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGRKDGMQTLDQHL
QELVDKGVISRQTARTKAVNKAAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=87639 MCIT9_RS05045 WP_317706319.1 951583..952617(-) (pilT) [Methylomarinovum caldicuralii strain IT-9]
ATGGACATCGCCGAACTGCTCGCCTTTTCCGTCAAGAACAATGCCTCCGACCTCCACCTCTCCGCCGGCCTGCCACCGAT
GATTCGCGTTGACGGCGACATCCGCCGCATCAACGTGCCGCCATTGGAGCACAAGGAGGTCCACGCGCTAGTTTACGACA
TCATGAACGACAAACAGCGGCGCGATTACGAGGAATACCTGGAAACCGACTTTTCCTTCGAGCTGCCCGGGGTGGCCCGT
TTCCGTGTCAACGCCTTCCATCAGGACCGGGGCGCGGCGGCGGTGTTCCGCACCATCCCCTCCAAGGTGCTGACCCTGGA
GGATCTGGGCTGCCCGCCGTTCTTCCGCGAAGTGACCCAGAAACCCCGCGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CCGGTAAGTCCACCACCCTGGCGGCGATGATCGACTACATCAACTCCAACAATTATTCCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTCCATCAGAGCAAGAAATCCCTCATCAACCAGCGCGAGGTCCACCGTGACACCCTCGGATTCAACGA
AGCCTTGAGATCGGCGCTGCGCGAGGACCCGGACGTGATCCTGGTGGGGGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCCTGACCGCGGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTGTTCCCGGCGGCGGAAAAAGCCATGATCCGTTCGATGCTGTCGGAATCCCTGCAGGCGGTCATCGCCCAGAC
CCTGCTGAAGAAGATCGGCGGCGGCAGGACCGCCGCCTGGGAGATCATGGTCGGAACCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCGATCCAGACCGGACGCAAGGACGGCATGCAGACCCTCGACCAGCACCTG
CAGGAACTGGTGGACAAGGGCGTCATCTCGCGCCAGACCGCCCGCACCAAGGCCGTCAACAAGGCGGCGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

81.395

100

0.814
  pilT Acinetobacter nosocomialis M2

81.395

100

0.814
  pilT Acinetobacter baumannii strain A118

81.395

100

0.814
  pilT Pseudomonas aeruginosa PAK

81.657

98.256

0.802
  pilT Pseudomonas stutzeri DSM 10701

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

76.744

100

0.767
  pilT Legionella pneumophila strain ERS1305867

76.744

100

0.767
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.03

97.965

0.686
  pilT Vibrio cholerae strain A1552

70.03

97.965

0.686
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392
  pilU Acinetobacter baylyi ADP1

40.361

96.512

0.39

Multiple sequence alignment