Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MCIT9_RS05040 Genome accession   NZ_AP024714
Coordinates   950447..951571 (-) Length   374 a.a.
NCBI ID   WP_317706318.1    Uniprot ID   A0AAU9BZ80
Organism   Methylomarinovum caldicuralii strain IT-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 945447..956571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCIT9_RS05010 (MIT9_P0964) - 945879..946703 (+) 825 WP_317706312.1 energy transducer TonB -
  MCIT9_RS05015 (MIT9_P0965) - 946715..947281 (+) 567 WP_317706313.1 YqgE/AlgH family protein -
  MCIT9_RS05020 (MIT9_P0966) ruvX 947274..947708 (+) 435 WP_317706314.1 Holliday junction resolvase RuvX -
  MCIT9_RS05025 (MIT9_P0967) pyrR 947705..948217 (+) 513 WP_317706315.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MCIT9_RS05030 (MIT9_P0968) - 948214..949173 (+) 960 WP_317706316.1 aspartate carbamoyltransferase catalytic subunit -
  MCIT9_RS05035 (MIT9_P0969) - 949170..950444 (+) 1275 WP_317706317.1 dihydroorotase -
  MCIT9_RS05040 (MIT9_P0970) pilU 950447..951571 (-) 1125 WP_317706318.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MCIT9_RS05045 (MIT9_P0971) pilT 951583..952617 (-) 1035 WP_317706319.1 type IV pilus twitching motility protein PilT Machinery gene
  MCIT9_RS05050 (MIT9_P0972) - 952646..953347 (+) 702 WP_317706320.1 YggS family pyridoxal phosphate-dependent enzyme -
  MCIT9_RS05055 (MIT9_P0973) - 953352..953699 (-) 348 WP_317706321.1 hypothetical protein -
  MCIT9_RS05060 (MIT9_P0974) - 953696..955582 (-) 1887 WP_317706322.1 nucleoside-diphosphate sugar epimerase/dehydratase -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 42246.46 Da        Isoelectric Point: 6.2902

>NTDB_id=87638 MCIT9_RS05040 WP_317706318.1 950447..951571(-) (pilU) [Methylomarinovum caldicuralii strain IT-9]
MNVESMLTLMVHKKASDLFVIAGRPPCIKVNGRLEPISPKSLSEPMARELVLSTMDSRQRDEFENARECNFAIAMENLAR
FRVSAYYQRNSPAMVIRRIQDYIPDVEELNLPPILKELAMTKRGLIIFVGATGTGKSTSLAAMLKYRNHNSSGHIITIED
PIEYTHQHAGCIVTQREVGTDTESYEVALKNTLRQAPDVILIGEIRTRETMQHAITFAETGHLCLSTLHANNANQAIDRI
LHFFPEDMHSQLFMDLSLNLKAIVAQQLIRRADGEGRYPAVEILINTPLASDLIRKGEVHKLKDLMKRSREQGMQTFDQC
LYDLYKAGKISYEDALAAADSRNEVRLMIKLGESGDLDRYAPGEDKMRLVEEDD

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=87638 MCIT9_RS05040 WP_317706318.1 950447..951571(-) (pilU) [Methylomarinovum caldicuralii strain IT-9]
ATGAACGTGGAATCCATGCTCACCCTGATGGTGCACAAGAAGGCCTCGGACCTGTTCGTGATCGCCGGCCGGCCGCCGTG
CATCAAGGTCAACGGCAGGCTGGAGCCCATCAGCCCCAAGTCCCTCAGCGAACCGATGGCGCGCGAGCTGGTGCTCAGCA
CCATGGACTCGCGCCAGCGGGACGAGTTCGAAAACGCCCGCGAGTGCAACTTCGCCATCGCCATGGAGAACCTGGCCCGC
TTCCGCGTCAGCGCCTACTACCAACGCAACAGCCCGGCGATGGTGATCCGGCGCATCCAGGATTACATCCCTGACGTGGA
GGAGCTGAACCTGCCGCCGATCCTTAAGGAGCTGGCAATGACCAAACGCGGTCTCATCATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCCCTGGCGGCGATGCTCAAATACCGCAACCACAACAGCTCCGGGCACATCATCACCATCGAGGAC
CCGATCGAGTACACCCACCAGCACGCCGGCTGCATCGTCACCCAGCGCGAGGTGGGCACCGACACCGAATCCTACGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCCCCGGACGTGATCCTCATCGGCGAGATCCGCACCCGGGAAACCATGCAGCACG
CCATCACCTTCGCCGAAACCGGGCATCTGTGCCTGTCCACTCTGCACGCCAACAACGCCAACCAAGCCATCGACCGCATC
CTGCACTTCTTCCCCGAGGACATGCACAGCCAGCTGTTCATGGACCTGTCCCTCAACCTCAAGGCCATCGTGGCCCAGCA
GCTGATCCGCCGCGCCGACGGCGAGGGCCGCTATCCGGCGGTGGAGATCCTCATCAACACCCCGCTGGCCTCGGACCTGA
TCCGCAAGGGCGAGGTCCACAAGCTCAAGGACCTGATGAAGCGCTCGCGCGAACAGGGGATGCAGACCTTCGACCAGTGC
CTCTACGATCTCTACAAGGCCGGCAAGATCAGCTACGAGGACGCCCTGGCCGCCGCCGACTCGCGCAACGAGGTGCGGCT
GATGATCAAACTGGGGGAAAGCGGCGACCTCGACCGTTACGCGCCCGGCGAAGACAAGATGCGGCTGGTGGAGGAGGACG
ACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.973

100

0.65
  pilU Acinetobacter baylyi ADP1

60.753

99.465

0.604
  pilU Vibrio cholerae strain A1552

55.372

97.059

0.537
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.241

93.048

0.393
  pilT Legionella pneumophila strain Lp02

42.773

90.642

0.388
  pilT Legionella pneumophila strain ERS1305867

42.773

90.642

0.388
  pilT Acinetobacter nosocomialis M2

39.823

90.642

0.361
  pilT Acinetobacter baumannii D1279779

39.823

90.642

0.361
  pilT Acinetobacter baumannii strain A118

39.823

90.642

0.361

Multiple sequence alignment