Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   TOL5_RS16915 Genome accession   NZ_AP024708
Coordinates   3625620..3626657 (+) Length   345 a.a.
NCBI ID   WP_005030845.1    Uniprot ID   A0A0A8TTJ0
Organism   Acinetobacter sp. Tol 5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3620620..3631657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL5_RS16890 (TOL5_33320) - 3620815..3621213 (-) 399 WP_042089392.1 hypothetical protein -
  TOL5_RS16895 (TOL5_33330) - 3621305..3622060 (-) 756 WP_058971186.1 hypothetical protein -
  TOL5_RS16900 (TOL5_33340) - 3622291..3623076 (-) 786 WP_221260520.1 hypothetical protein -
  TOL5_RS16905 (TOL5_33350) - 3623255..3624088 (-) 834 WP_058971179.1 undecaprenyl-diphosphate phosphatase -
  TOL5_RS16910 (TOL5_33360) - 3624481..3625173 (-) 693 WP_198260401.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOL5_RS16915 (TOL5_33370) pilT 3625620..3626657 (+) 1038 WP_005030845.1 type IV pilus twitching motility protein PilT Machinery gene
  TOL5_RS16920 (TOL5_33380) pilU 3626685..3627803 (+) 1119 WP_121773981.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOL5_RS16925 (TOL5_33390) fur 3627878..3628315 (-) 438 WP_004828050.1 ferric iron uptake transcriptional regulator -
  TOL5_RS16930 (TOL5_33400) - 3628428..3628826 (+) 399 WP_004828048.1 outer membrane protein assembly factor BamE -
  TOL5_RS16935 (TOL5_33410) - 3628908..3629231 (-) 324 WP_009585850.1 RnfH family protein -
  TOL5_RS16940 (TOL5_33420) - 3629249..3630331 (-) 1083 WP_199934884.1 hypothetical protein -
  TOL5_RS16945 (TOL5_33430) - 3630532..3630987 (-) 456 WP_004828043.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38254.96 Da        Isoelectric Point: 6.5055

>NTDB_id=87594 TOL5_RS16915 WP_005030845.1 3625620..3626657(+) (pilT) [Acinetobacter sp. Tol 5]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALEHKEVHRLVYDIMNDKQRRDFEEQLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDLCDLPRGLILVTGPTGSGKSTTLASMIDFINDNRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KALVAKGVISPQVARNVAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=87594 TOL5_RS16915 WP_005030845.1 3625620..3626657(+) (pilT) [Acinetobacter sp. Tol 5]
ATGGATATTACAGAATTATTGGCGTTTTCCGTTAAAAATGGTGCGTCTGACTTACACCTTTCAGCGGGTATGCCACCGAT
GATTCGTGTTGATGGTGAAGTCCGCCGTATCAATTTACCAGCATTGGAACATAAAGAAGTTCATAGACTGGTCTATGACA
TCATGAATGACAAACAACGTCGAGATTTTGAAGAACAATTAGAGACTGACTTCTCTTTTGAAGTTCCAGGCGTTGCCCGT
TTCCGTGTTAACGCATTTAACCAAAACCGTGGTGCAGGTGCGGTATTCCGTACTATTCCATCCAAAGTCCTGACTATGGA
GGATCTTGGTTTAGGACAAATCTTTAAAGACCTTTGTGACTTACCACGCGGTTTAATTTTGGTTACAGGTCCAACAGGTT
CTGGTAAATCAACCACTTTGGCTTCGATGATTGACTTCATCAATGACAATCGTTATGACCATATTTTAACTGTCGAAGAT
CCAATCGAATTTGTACACCAATCTAAAAAGTGCTTGATCAACCAGCGCGAAGTGCATCGTGATACACATGGCTTTAACGA
GGCGCTACGTTCTGCACTGCGTGAAGATCCAGACATTATCCTTGTCGGTGAGATGCGTGACCTTGAAACTATCCGCCTTG
CACTTACCGCAGCAGAAACAGGTCACTTGGTCTTTGGTACACTGCATACCACTTCCGCAGCGAAGACCATTGACCGTGTG
ATTGATGTATTCCCTGCTGAAGAAAAAGACATGGTACGTGCCATGCTTTCTGAATCATTGCAGGCGGTAATTTCTCAAAC
GCTATTAAAGAAAAATGGCGGTGGACGTGTTGCTGCACATGAAATCATGATCGGGATTCCTGCAATTCGTAACCTTATTC
GTGAAAACAAAGTTGCACAGATGTATTCAGCGATCCAGACTGGCGCAAACTATGGTATGACCACACTGGATCAAAGCTTA
AAAGCGCTGGTTGCCAAAGGTGTAATTAGTCCTCAAGTTGCGCGAAATGTTGCAAAACAACCAGAATCATTTCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A8TTJ0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

94.783

100

0.948
  pilT Acinetobacter baumannii D1279779

94.783

100

0.948
  pilT Acinetobacter nosocomialis M2

94.493

100

0.945
  pilT Acinetobacter baylyi ADP1

86.919

99.71

0.867
  pilT Pseudomonas aeruginosa PAK

84.012

99.71

0.838
  pilT Pseudomonas stutzeri DSM 10701

83.43

99.71

0.832
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.578

96.232

0.67
  pilT Vibrio cholerae strain A1552

69.578

96.232

0.67
  pilT Neisseria gonorrhoeae MS11

66.377

100

0.664
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

100

0.516
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Acinetobacter baylyi ADP1

39.884

100

0.4
  pilU Pseudomonas stutzeri DSM 10701

41.39

95.942

0.397

Multiple sequence alignment