Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   TOL5_RS16920 Genome accession   NZ_AP024708
Coordinates   3626685..3627803 (+) Length   372 a.a.
NCBI ID   WP_121773981.1    Uniprot ID   -
Organism   Acinetobacter sp. Tol 5     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3621685..3632803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TOL5_RS16900 (TOL5_33340) - 3622291..3623076 (-) 786 WP_221260520.1 hypothetical protein -
  TOL5_RS16905 (TOL5_33350) - 3623255..3624088 (-) 834 WP_058971179.1 undecaprenyl-diphosphate phosphatase -
  TOL5_RS16910 (TOL5_33360) - 3624481..3625173 (-) 693 WP_198260401.1 YggS family pyridoxal phosphate-dependent enzyme -
  TOL5_RS16915 (TOL5_33370) pilT 3625620..3626657 (+) 1038 WP_005030845.1 type IV pilus twitching motility protein PilT Machinery gene
  TOL5_RS16920 (TOL5_33380) pilU 3626685..3627803 (+) 1119 WP_121773981.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  TOL5_RS16925 (TOL5_33390) fur 3627878..3628315 (-) 438 WP_004828050.1 ferric iron uptake transcriptional regulator -
  TOL5_RS16930 (TOL5_33400) - 3628428..3628826 (+) 399 WP_004828048.1 outer membrane protein assembly factor BamE -
  TOL5_RS16935 (TOL5_33410) - 3628908..3629231 (-) 324 WP_009585850.1 RnfH family protein -
  TOL5_RS16940 (TOL5_33420) - 3629249..3630331 (-) 1083 WP_199934884.1 hypothetical protein -
  TOL5_RS16945 (TOL5_33430) - 3630532..3630987 (-) 456 WP_004828043.1 bacteriohemerythrin -
  TOL5_RS16950 (TOL5_33440) - 3631272..3632120 (-) 849 WP_121774176.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 372 a.a.        Molecular weight: 41839.18 Da        Isoelectric Point: 6.4215

>NTDB_id=87595 TOL5_RS16920 WP_121773981.1 3626685..3627803(+) (pilU) [Acinetobacter sp. Tol 5]
MDFNDLLNMMIEQKASDLFITADVEPSMKINGQIVPVVKTKLNGDIIMQLLNSIMSEKQRKEFADTRECNFAITNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPTMEELKLPPILKDLAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFVHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFESDRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLQIKLDEEGGAQLLNANSKLTFDGQ

Nucleotide


Download         Length: 1119 bp        

>NTDB_id=87595 TOL5_RS16920 WP_121773981.1 3626685..3627803(+) (pilU) [Acinetobacter sp. Tol 5]
ATGGACTTTAATGACTTGCTCAATATGATGATTGAGCAGAAAGCATCCGACTTGTTTATTACCGCAGATGTAGAACCATC
AATGAAAATTAATGGTCAAATCGTCCCTGTGGTAAAAACCAAGTTAAACGGCGACATCATCATGCAATTGCTCAACTCAA
TCATGAGTGAAAAGCAACGCAAAGAATTTGCAGATACGCGCGAATGCAACTTTGCCATTACCAACAGAGATAAAACTGCC
CGTTTCCGTGTTAGCGCTTTTCAGCAACGTGATATGCCAGGTATGGTTTTGCGTCGTATCGAAACTAAAATTCCAACCAT
GGAAGAGTTAAAACTTCCACCTATACTCAAAGATCTTGCCATGACAAAACGTGGCATCATTATCTTTGTTGGTGCGACAG
GTACCGGTAAATCCACTTCTCTTGCATCGATTATTGGCTATCGCAATCAGAACTCAAAAGGCCATATCATCACCATTGAA
GATCCGATCGAATTTGTTCACGAACATGCTGGCTGTATCATCACCCAACGTGAAGTTGGTATTGATACTGACTCTTTTGA
AATTGCTTTGAAGAACACCTTGCGTCAAGCACCTGATGTTATTCTGATTGGTGAGATTCGTTCCCGTGAAACAATGGATT
ATGCGATTGCCTTTGCAGAAACTGGACATCTGGTCTTTGCGACACTACATGCCAACAATGCCAACCAAGCAATTGACCGC
ATTATTCACTTCTTTGAATCTGACCGTCATAGCCAATTGTTTATGGACTTATCTCTTAACCTTAAAGCTATGGTCGCACA
GCAATTAATTCCAACACCAGATGGTAACTCGCGTCGTGCAGCGATTGAGATTCTGATCAACTCACCACTCGTTTCAGATC
TCATCCGTAAAGGTGATGTACATGAAATCAAAGATTTGATGAAACGTTCACGCGAATTGGGTATGCAAACTTTTGACCAA
GCCTTGTTTGACCTTTATAAAGCTGGTCAGATTACCTACAAAGATGCGTTGAAACATGCAGACTCACCAAATGATTTACG
TCTACAAATCAAATTGGATGAAGAAGGTGGCGCGCAGTTGTTGAATGCCAACAGCAAATTGACTTTTGATGGGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.455

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

68.194

99.731

0.68
  pilU Vibrio cholerae strain A1552

55.342

98.118

0.543
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

93.011

0.401
  pilT Legionella pneumophila strain ERS1305867

40.828

90.86

0.371
  pilT Legionella pneumophila strain Lp02

40.828

90.86

0.371
  pilT Acinetobacter nosocomialis M2

39.942

92.204

0.368
  pilT Acinetobacter baumannii strain A118

39.942

92.204

0.368
  pilT Acinetobacter baumannii D1279779

39.942

92.204

0.368
  pilT Pseudomonas aeruginosa PAK

39.595

93.011

0.368
  pilT Acinetobacter baylyi ADP1

39.359

92.204

0.363
  pilT Neisseria gonorrhoeae MS11

39.412

91.398

0.36
  pilT Pseudomonas stutzeri DSM 10701

38.728

93.011

0.36
  pilT Neisseria meningitidis 8013

38.728

93.011

0.36

Multiple sequence alignment