Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCV51_RS08235 Genome accession   NZ_CP133611
Coordinates   1750634..1751785 (-) Length   383 a.a.
NCBI ID   WP_013178812.1    Uniprot ID   D7CSM9
Organism   Truepera radiovictrix strain CIP 108686     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1745634..1756785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCV51_RS08210 (RCV51_08210) - 1746068..1746847 (+) 780 WP_041947275.1 alpha-hydroxy-acid oxidizing protein -
  RCV51_RS08215 (RCV51_08215) - 1746899..1747675 (-) 777 WP_148221243.1 hypothetical protein -
  RCV51_RS08220 (RCV51_08220) - 1747672..1748493 (-) 822 WP_245523541.1 bifunctional diguanylate cyclase/phosphodiesterase -
  RCV51_RS08225 (RCV51_08225) - 1748515..1748868 (-) 354 Protein_1624 diguanylate cyclase domain-containing protein -
  RCV51_RS08230 (RCV51_08230) - 1748765..1750468 (-) 1704 WP_083770816.1 CHASE4 domain-containing protein -
  RCV51_RS08235 (RCV51_08235) pilT 1750634..1751785 (-) 1152 WP_013178812.1 type IV pilus twitching motility protein PilT Machinery gene
  RCV51_RS08240 (RCV51_08240) pilF 1751823..1754474 (-) 2652 WP_013178811.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RCV51_RS08245 (RCV51_08245) - 1754456..1755046 (-) 591 WP_013178810.1 YqeG family HAD IIIA-type phosphatase -
  RCV51_RS08250 (RCV51_08250) - 1755100..1756041 (-) 942 WP_013178809.1 alpha/beta fold hydrolase -
  RCV51_RS08255 (RCV51_08255) - 1756038..1756280 (-) 243 WP_041947274.1 hypothetical protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42029.25 Da        Isoelectric Point: 9.3771

>NTDB_id=875291 RCV51_RS08235 WP_013178812.1 1750634..1751785(-) (pilT) [Truepera radiovictrix strain CIP 108686]
MSRATPEIIPLLRLASERRASDLLLTTGLPPMLHLDGAWQPTEFEPLTANDTRRLMYALMDEKKQRDFEERRELDFSFML
SGHGRFRVNAFFQRGAVGGVLRTIASELPSLERLGLPQVVADVIKQPRGLVLVTGPTGSGKSTTLAALIDRINTESRKHI
ITIEDPIEFFHPHKRSIVNQRELGEDTKSFERALRAVLRQAPDVILVGEMRDFETIGAAVTAAETGHLVIATLHTNSAPE
AVDRIIDVFPEAQQAQVRVQLASNLQAILTQQLVPKVGGGRVLAYEFLVATPAVRNLIREGKTHQIPSAIQMGGQYGMQT
MDAHLAELHARRLITYEAGLTRAVDPKEFARLVSSHGPGAPAPDARRAEGPPLPPFGRGGRGA

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=875291 RCV51_RS08235 WP_013178812.1 1750634..1751785(-) (pilT) [Truepera radiovictrix strain CIP 108686]
ATGAGCCGAGCGACACCCGAGATCATCCCCCTGCTGCGCCTAGCGAGCGAACGCCGCGCCTCCGACCTGCTGCTCACGAC
CGGCCTCCCCCCGATGCTGCACCTCGACGGCGCGTGGCAGCCAACCGAGTTTGAACCGCTCACCGCCAACGACACCCGGC
GGCTGATGTACGCCCTTATGGACGAGAAAAAGCAGCGCGACTTCGAGGAGCGGCGTGAGCTCGACTTCTCCTTTATGCTG
AGCGGGCACGGCCGCTTCCGCGTCAACGCCTTTTTCCAACGCGGCGCCGTCGGCGGGGTTTTGCGCACGATCGCCAGCGA
GCTCCCCAGCCTCGAGCGCCTCGGCCTCCCCCAGGTCGTCGCCGACGTCATCAAGCAGCCGCGCGGCCTCGTGCTCGTCA
CCGGACCGACCGGCTCCGGCAAATCGACGACGCTCGCTGCGCTCATCGACCGCATCAACACCGAGTCGCGCAAACACATC
ATCACCATCGAAGACCCCATCGAGTTCTTCCACCCTCACAAACGCTCGATCGTCAACCAGCGCGAGCTCGGCGAGGACAC
GAAGAGCTTTGAACGCGCCCTGCGGGCGGTTTTGCGCCAGGCTCCGGATGTGATCTTGGTCGGCGAGATGCGCGACTTCG
AGACCATCGGGGCGGCCGTCACGGCCGCCGAGACCGGACACCTGGTCATCGCCACGTTGCACACCAACTCCGCCCCCGAA
GCGGTCGACCGCATCATCGACGTCTTCCCCGAAGCGCAGCAGGCGCAGGTGCGGGTGCAGCTCGCGAGCAACCTCCAGGC
GATCTTGACGCAGCAGCTCGTCCCCAAAGTCGGCGGCGGGCGGGTGCTGGCCTACGAGTTTCTCGTCGCCACGCCCGCCG
TGCGCAACCTTATCCGCGAAGGCAAAACGCACCAGATCCCCTCGGCGATCCAGATGGGCGGCCAGTACGGGATGCAGACC
ATGGACGCGCACCTCGCCGAGCTGCACGCGCGGCGCCTGATCACGTACGAGGCGGGCCTGACTCGAGCCGTCGACCCGAA
GGAGTTCGCGCGGCTCGTGAGCAGCCACGGCCCCGGCGCCCCCGCCCCCGACGCCCGGCGCGCCGAAGGCCCCCCCCTAC
CACCTTTCGGACGGGGCGGGCGCGGCGCTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(49-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7CSM9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

62.663

100

0.627
  pilT Pseudomonas aeruginosa PAK

52.478

89.556

0.47
  pilT Acinetobacter baumannii strain A118

51.312

89.556

0.46
  pilT Acinetobacter baumannii D1279779

51.312

89.556

0.46
  pilT Pseudomonas stutzeri DSM 10701

51.312

89.556

0.46
  pilT Acinetobacter nosocomialis M2

51.02

89.556

0.457
  pilT Acinetobacter baylyi ADP1

50.437

89.556

0.452
  pilT Legionella pneumophila strain ERS1305867

50

89.295

0.446
  pilT Legionella pneumophila strain Lp02

50

89.295

0.446
  pilT Neisseria gonorrhoeae MS11

48.116

90.078

0.433
  pilT Neisseria meningitidis 8013

48.116

90.078

0.433
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

85.64

0.431
  pilT Vibrio cholerae strain A1552

50.305

85.64

0.431
  pilU Pseudomonas stutzeri DSM 10701

47.929

88.251

0.423
  pilU Vibrio cholerae strain A1552

45.659

81.201

0.371
  pilU Acinetobacter baylyi ADP1

41.056

89.034

0.366