Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   RCV51_RS08240 Genome accession   NZ_CP133611
Coordinates   1751823..1754474 (-) Length   883 a.a.
NCBI ID   WP_013178811.1    Uniprot ID   D7CSM8
Organism   Truepera radiovictrix strain CIP 108686     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1746823..1759474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCV51_RS08215 (RCV51_08215) - 1746899..1747675 (-) 777 WP_148221243.1 hypothetical protein -
  RCV51_RS08220 (RCV51_08220) - 1747672..1748493 (-) 822 WP_245523541.1 bifunctional diguanylate cyclase/phosphodiesterase -
  RCV51_RS08225 (RCV51_08225) - 1748515..1748868 (-) 354 Protein_1624 diguanylate cyclase domain-containing protein -
  RCV51_RS08230 (RCV51_08230) - 1748765..1750468 (-) 1704 WP_083770816.1 CHASE4 domain-containing protein -
  RCV51_RS08235 (RCV51_08235) pilT 1750634..1751785 (-) 1152 WP_013178812.1 type IV pilus twitching motility protein PilT Machinery gene
  RCV51_RS08240 (RCV51_08240) pilF 1751823..1754474 (-) 2652 WP_013178811.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  RCV51_RS08245 (RCV51_08245) - 1754456..1755046 (-) 591 WP_013178810.1 YqeG family HAD IIIA-type phosphatase -
  RCV51_RS08250 (RCV51_08250) - 1755100..1756041 (-) 942 WP_013178809.1 alpha/beta fold hydrolase -
  RCV51_RS08255 (RCV51_08255) - 1756038..1756280 (-) 243 WP_041947274.1 hypothetical protein -
  RCV51_RS08260 (RCV51_08260) - 1756813..1757235 (+) 423 WP_013178807.1 hypothetical protein -
  RCV51_RS08265 (RCV51_08265) - 1757222..1757998 (-) 777 WP_013178806.1 TatD family hydrolase -
  RCV51_RS08270 (RCV51_08270) - 1758231..1758551 (+) 321 WP_041947273.1 hypothetical protein -
  RCV51_RS15255 - 1758588..1758938 (+) 351 Protein_1634 septal ring lytic transglycosylase RlpA family protein -

Sequence

Protein


Download         Length: 883 a.a.        Molecular weight: 96468.67 Da        Isoelectric Point: 5.1049

>NTDB_id=875292 RCV51_RS08240 WP_013178811.1 1751823..1754474(-) (pilF) [Truepera radiovictrix strain CIP 108686]
MAVLSIGDKRLGAMLLERGFISDEGLQRAIARQAETGGRLSEVLINLGLLSEQRIARAIEEAMGVPLVHLTRTPTDPQAL
AKVPAALALELRALPFALQGTRLRVAFEDPLDALAAEELEDVSGCIVEVFQALGSELTWALATHYPELGLTPPAELNPAL
GSRVGELALTLGLITREQLEAALAQQARTGGLLGSILQEQGALSDVQLAELLAQQADLPFTADLRGARISERLASHLLRV
DALQYRAVPYQEEADGLVVALADPRRADDLREIIRTPVRFVVAPESQVQALTDALYEHDKGRLGETLLQAGKLRREQLRE
ALYEQQKRGRVRPLGDILVDLGFVSREDVQWALAEQRTRGGRLEDTLVQAGRISPEMLSRSLALQLGYAFVEENVQVDAA
AVALVPEGAVRRYNVMPLRVEGNTLTLAMKDPRHVFAIDDIRLLTGKEIRPAVATEETLIKLINRHYKSNENMEELAKAV
LEEIGDQDEIEDDSHLDDTALVKVVNNIIREAVFNDISDIHIEPYPDKVLVRVRKDGALREYMSLPRGVAGAVAARIKIM
GRLNIAERRLPQDGRVRFKDKNLEVDLRLSTLPTVYGEKVVMRLLKRASAIPDIEQLGFADYNFERFTDTIQKPYGIFLI
TGPTGSGKSFTTFSILKRIATPDVNVTTVEDPVEYEIPGINQTQVNVKAGLTFASALRAFLRQDPDVIMIGEIRDTETAK
IAVEAALTGHLVIATLHTNDATGAVTRLAEMGVEPFNISASLLGVLAQRLVRKVCAACRVAYTPDVDTLRRLGLAPEALQ
GATLYRGVGCTQCGGSGYNGRHAIHELFTVDAAAQRAIVQGRSSSELKELAVAAGMKTLRDDGVFKALAGITTLEEVLAR
TTD

Nucleotide


Download         Length: 2652 bp        

>NTDB_id=875292 RCV51_RS08240 WP_013178811.1 1751823..1754474(-) (pilF) [Truepera radiovictrix strain CIP 108686]
GTGGCCGTCCTGTCAATAGGCGACAAACGCCTCGGCGCCATGCTTTTAGAGCGCGGCTTTATCAGCGATGAGGGGTTGCA
ACGCGCCATCGCGCGCCAGGCCGAAACGGGGGGGCGCCTCTCGGAGGTGCTCATCAACCTGGGGCTCTTGTCCGAGCAGC
GCATCGCGCGCGCCATCGAGGAGGCGATGGGGGTGCCGCTGGTGCACCTGACCCGCACCCCCACCGACCCCCAAGCGCTC
GCCAAGGTCCCGGCGGCGCTCGCTCTTGAGCTGCGCGCGCTGCCCTTCGCCCTGCAGGGGACGCGCCTACGGGTCGCTTT
CGAGGACCCTTTAGACGCGCTCGCCGCCGAGGAGCTCGAGGACGTCAGCGGCTGCATCGTCGAGGTCTTTCAGGCGCTCG
GCAGCGAACTCACCTGGGCGCTCGCCACCCACTACCCCGAACTCGGCCTCACCCCCCCCGCCGAACTCAACCCCGCGCTG
GGGTCGCGCGTCGGCGAGCTCGCGCTCACCTTAGGGCTCATCACCCGCGAGCAGCTCGAGGCCGCGTTAGCGCAGCAGGC
GCGTACGGGGGGGCTTTTGGGCAGCATCTTGCAGGAGCAGGGGGCGCTAAGCGACGTGCAGCTCGCCGAACTGCTCGCGC
AGCAAGCCGACCTGCCCTTTACCGCCGACCTGCGCGGCGCGCGTATCAGCGAACGGCTCGCCTCGCACCTGCTGCGCGTC
GACGCGCTGCAGTACCGCGCGGTGCCCTACCAAGAGGAGGCGGACGGTCTTGTCGTGGCGCTCGCCGATCCGCGCCGCGC
GGACGACCTTCGGGAGATTATCCGCACCCCCGTGCGCTTCGTCGTCGCCCCGGAGAGCCAGGTGCAGGCGCTCACCGACG
CCCTTTACGAACACGACAAGGGGCGGCTCGGCGAGACGTTGCTGCAAGCGGGCAAGCTGCGGCGCGAACAGCTGCGCGAG
GCGCTCTACGAGCAGCAAAAGCGCGGGCGCGTAAGGCCGCTCGGCGACATCTTGGTCGACCTCGGCTTTGTCAGCCGCGA
GGACGTGCAGTGGGCGCTCGCCGAGCAGCGCACGCGCGGGGGGCGGCTCGAGGACACCCTCGTGCAAGCGGGGCGCATCA
GCCCCGAGATGCTCTCGCGCAGCCTCGCGCTGCAACTCGGCTACGCCTTCGTCGAGGAGAACGTGCAGGTCGACGCGGCG
GCGGTCGCGCTCGTCCCCGAGGGGGCGGTGCGCCGCTACAACGTGATGCCGCTGCGCGTCGAGGGCAACACCCTCACGCT
CGCCATGAAAGACCCGCGGCACGTCTTCGCCATCGACGACATCCGGCTCCTCACGGGTAAAGAGATCCGCCCCGCGGTCG
CTACCGAAGAGACCCTCATTAAGCTGATCAACCGCCACTACAAGAGCAACGAGAACATGGAGGAGCTCGCCAAGGCGGTT
TTAGAGGAGATCGGCGACCAAGACGAGATTGAAGACGACTCCCACTTAGACGACACCGCGCTCGTCAAGGTCGTCAACAA
CATCATCCGCGAGGCGGTCTTCAACGACATCTCGGACATCCACATCGAACCCTACCCCGACAAGGTGCTCGTGCGGGTCC
GCAAAGACGGCGCGCTCCGCGAGTACATGTCGTTGCCGCGCGGCGTCGCCGGCGCGGTCGCGGCGCGCATCAAGATCATG
GGGCGCCTTAATATCGCCGAGAGGCGGCTGCCGCAAGACGGGCGGGTGCGCTTTAAAGACAAGAACCTCGAGGTCGACCT
GCGCCTCTCGACGCTGCCCACGGTCTACGGCGAAAAGGTCGTGATGCGCCTTTTAAAGCGCGCCTCGGCAATTCCCGACA
TCGAGCAGCTCGGTTTCGCCGACTACAACTTCGAGCGCTTTACCGACACCATCCAAAAGCCCTACGGCATTTTTCTCATC
ACCGGGCCGACCGGCTCGGGCAAGTCGTTTACCACCTTCTCCATCTTAAAGCGCATCGCCACCCCGGACGTCAACGTCAC
GACGGTCGAGGACCCCGTCGAGTACGAGATCCCGGGGATCAACCAGACGCAGGTCAACGTCAAAGCGGGGCTCACCTTCG
CGAGCGCCCTGCGCGCGTTTTTACGCCAAGACCCGGACGTGATCATGATCGGCGAGATCCGCGACACCGAAACCGCCAAG
ATCGCCGTCGAGGCGGCGCTGACCGGCCACCTGGTGATCGCCACGCTGCACACCAACGACGCCACGGGGGCGGTCACGCG
GCTCGCGGAGATGGGGGTCGAACCCTTTAACATCTCCGCGTCGCTCTTGGGGGTGCTCGCGCAGCGGCTCGTGCGCAAGG
TGTGCGCCGCTTGCCGCGTCGCCTACACCCCCGACGTGGACACCCTGCGGCGTTTGGGGCTCGCCCCCGAAGCGCTCCAG
GGCGCGACGCTCTACCGCGGCGTCGGCTGCACGCAGTGTGGCGGCAGCGGCTACAACGGCCGCCACGCGATCCACGAACT
GTTTACCGTCGACGCCGCGGCGCAGCGCGCCATCGTGCAGGGCCGCTCGTCGAGCGAACTCAAAGAGCTCGCGGTCGCTG
CGGGGATGAAGACGCTGCGCGACGACGGGGTGTTCAAGGCGCTCGCCGGCATCACCACGCTCGAAGAGGTGCTCGCGCGC
ACCACCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(507-773)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(197-300)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(367-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D7CSM8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

56.887

100

0.575
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.576

100

0.547