Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG17_RS06045 Genome accession   NZ_CP133268
Coordinates   1155390..1156430 (-) Length   346 a.a.
NCBI ID   WP_308174341.1    Uniprot ID   -
Organism   Neobacillus sp. PS3-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1150390..1161430
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG17_RS06020 (RCG17_06020) - 1151074..1151739 (-) 666 WP_308174336.1 hypothetical protein -
  RCG17_RS06025 (RCG17_06025) pilM 1151743..1152729 (-) 987 WP_308174337.1 pilus assembly protein PilM -
  RCG17_RS06030 (RCG17_06030) comC 1152755..1153510 (-) 756 WP_308174338.1 prepilin peptidase Machinery gene
  RCG17_RS06035 (RCG17_06035) - 1153516..1154028 (-) 513 WP_308174339.1 type II secretion system protein -
  RCG17_RS06040 (RCG17_06040) - 1154174..1155388 (-) 1215 WP_308174340.1 type II secretion system F family protein -
  RCG17_RS06045 (RCG17_06045) pilT 1155390..1156430 (-) 1041 WP_308174341.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG17_RS06050 (RCG17_06050) - 1156444..1158102 (-) 1659 WP_308174342.1 GspE/PulE family protein -
  RCG17_RS06055 (RCG17_06055) - 1158145..1159080 (-) 936 WP_308174343.1 G5 domain-containing protein -
  RCG17_RS06060 (RCG17_06060) - 1159073..1159909 (-) 837 WP_308174344.1 hypothetical protein -
  RCG17_RS06065 (RCG17_06065) - 1160237..1160728 (+) 492 WP_308174345.1 hypothetical protein -
  RCG17_RS06070 (RCG17_06070) - 1160733..1161209 (+) 477 WP_308174346.1 type II secretion system protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38381.53 Da        Isoelectric Point: 6.8343

>NTDB_id=873082 RCG17_RS06045 WP_308174341.1 1155390..1156430(-) (pilT) [Neobacillus sp. PS3-12]
MNERIDLLLRAAFELKASDVHLTVGVPPIMRINGDLKRYGKEVLSSEDTEGMAKAIVPVQMWDVFKQKGELDFSYSIAGV
SRFRVNTFHQRSCISLSLRIVPTKIPTIQELDLPEVLTKIIELPQGLILVTGPTGSGKSTTLAAMIDYMNKTMRKHVITL
EDPIEYLHKHGNCIIDQREVGFDTSNFSNGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLATLHTSGAPATIN
RIIDVFSPAQQQQIRIQLASVLVGIVSQRLFQTIDHKSRVAATEIMINNPAIANLIRNEKVHQIISVMQTSKAQGMHTLE
SSIKELIDRGFISIEAAAPYMQEILI

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=873082 RCG17_RS06045 WP_308174341.1 1155390..1156430(-) (pilT) [Neobacillus sp. PS3-12]
ATGAATGAAAGAATTGATCTTTTGTTGCGGGCCGCTTTTGAGTTGAAGGCGTCAGATGTCCATCTGACCGTTGGTGTTCC
TCCAATTATGCGGATTAATGGTGACTTAAAAAGATATGGAAAGGAAGTATTAAGCTCAGAAGATACAGAAGGAATGGCCA
AGGCGATTGTTCCCGTACAGATGTGGGATGTCTTTAAGCAAAAAGGAGAACTCGACTTCTCCTACAGCATTGCTGGTGTT
TCCCGTTTCCGTGTCAACACATTTCATCAGCGTTCCTGTATTTCGCTGTCATTACGGATCGTTCCGACAAAGATCCCTAC
TATACAGGAATTGGATTTACCTGAAGTTCTTACAAAGATAATCGAACTGCCTCAGGGGTTAATCCTGGTAACGGGACCCA
CAGGAAGCGGAAAATCAACCACGCTTGCAGCAATGATTGATTATATGAATAAAACCATGCGCAAACATGTAATTACTCTT
GAGGACCCTATTGAATACCTTCACAAACATGGGAACTGTATTATTGATCAACGAGAAGTTGGATTTGATACCTCTAATTT
TTCCAATGGCTTGCGGGCTGCTCTCCGTCAGGACCCTGATGTCATTCTCGTCGGTGAGATGCGGGATTTGGAGACGATTC
AAACAGCCATTACAGCAGCAGAAACAGGACATTTGGTGTTAGCCACTCTGCATACATCAGGTGCACCGGCAACGATCAAC
CGCATAATTGATGTTTTCTCACCGGCACAGCAGCAGCAAATCCGGATTCAGCTTGCCTCCGTTTTAGTGGGGATTGTCTC
ACAACGCCTGTTCCAAACCATTGATCATAAAAGCAGAGTTGCTGCAACTGAAATCATGATTAATAATCCTGCGATTGCAA
ACTTAATCCGTAACGAAAAGGTACACCAGATCATCAGTGTGATGCAAACTTCCAAGGCCCAAGGAATGCATACACTCGAA
TCAAGCATTAAAGAATTAATTGATCGGGGATTCATTTCAATAGAAGCTGCCGCTCCTTATATGCAGGAGATTTTAATTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.555

97.399

0.483
  pilT Vibrio cholerae strain A1552

49.538

93.931

0.465
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.538

93.931

0.465
  pilT Pseudomonas aeruginosa PAK

48.78

94.798

0.462
  pilT Pseudomonas stutzeri DSM 10701

48.476

94.798

0.46
  pilT Acinetobacter baumannii D1279779

47.866

94.798

0.454
  pilT Acinetobacter nosocomialis M2

47.866

94.798

0.454
  pilT Acinetobacter baumannii strain A118

47.866

94.798

0.454
  pilT Neisseria meningitidis 8013

47.289

95.954

0.454
  pilT Neisseria gonorrhoeae MS11

47.289

95.954

0.454
  pilT Acinetobacter baylyi ADP1

46.364

95.376

0.442
  pilT Legionella pneumophila strain ERS1305867

45.455

95.376

0.434
  pilT Legionella pneumophila strain Lp02

45.455

95.376

0.434
  pilU Pseudomonas stutzeri DSM 10701

44.242

95.376

0.422
  pilU Acinetobacter baylyi ADP1

39.521

96.532

0.382
  pilU Vibrio cholerae strain A1552

41.346

90.173

0.373