Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG24_RS01135 Genome accession   NZ_CP133263
Coordinates   220391..221431 (+) Length   346 a.a.
NCBI ID   WP_202652287.1    Uniprot ID   -
Organism   Neobacillus sp. OS1-32     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 215391..226431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG24_RS01115 (RCG24_01115) - 215483..217534 (-) 2052 WP_308078111.1 hypothetical protein -
  RCG24_RS01120 (RCG24_01120) - 217549..218127 (-) 579 WP_202652275.1 PilW family protein -
  RCG24_RS01125 (RCG24_01125) - 218272..218697 (+) 426 Protein_197 hypothetical protein -
  RCG24_RS01130 (RCG24_01130) - 218715..220379 (+) 1665 WP_202652285.1 GspE/PulE family protein -
  RCG24_RS01135 (RCG24_01135) pilT 220391..221431 (+) 1041 WP_202652287.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG24_RS01140 (RCG24_01140) - 221433..222638 (+) 1206 WP_202652289.1 type II secretion system F family protein -
  RCG24_RS01145 (RCG24_01145) - 222745..223209 (+) 465 WP_308078113.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG24_RS01150 (RCG24_01150) comC 223211..223954 (+) 744 WP_308078115.1 prepilin peptidase Machinery gene
  RCG24_RS01155 (RCG24_01155) pilM 223967..224974 (+) 1008 WP_308078116.1 pilus assembly protein PilM -
  RCG24_RS01160 (RCG24_01160) - 224974..225621 (+) 648 WP_202652297.1 fimbrial assembly protein -
  RCG24_RS01165 (RCG24_01165) - 225618..226373 (+) 756 WP_202652299.1 pilus assembly protein PilO -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38041.20 Da        Isoelectric Point: 8.9987

>NTDB_id=872881 RCG24_RS01135 WP_202652287.1 220391..221431(+) (pilT) [Neobacillus sp. OS1-32]
MKSKIDLLLKAGFDVKASDIHLTVGVPPIVRINGELKRLGKEPLTPVDTEEMAKAIVPSGMWERFKEKGELDFSYGLPGV
SRFRVNAYFQRTCIAMAIRVIPTRIPTLDELDLPAIIKKIADRPHGLVLVTGPTGSGKSTTLAALLNFMNQTQRKHIVTL
EDPIEYLHKHGNCIIDQREVGFDTETFANGLRAALRQDPDVILVGEMRDLETIQTAITAAETGHLVLGTLHTSSAPATIN
RIIDVFPPSQQPQIKIQLASVLVAIVSQRLFPTADRKGRRAATEILVNNSAVANLIRNDKIHQIANVMQTSRASGMQTLE
MNIKDLLQSGAIAKEAVVPFLQEKVE

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=872881 RCG24_RS01135 WP_202652287.1 220391..221431(+) (pilT) [Neobacillus sp. OS1-32]
ATGAAGAGTAAAATTGACCTTCTGCTTAAAGCCGGGTTTGATGTAAAGGCATCGGATATTCATTTGACAGTTGGGGTTCC
GCCGATTGTAAGAATCAATGGTGAATTAAAAAGACTTGGCAAAGAGCCGCTAACTCCTGTTGATACCGAAGAAATGGCAA
AGGCAATTGTCCCCAGCGGGATGTGGGAAAGATTTAAGGAAAAAGGAGAGCTTGATTTTTCTTATGGACTGCCAGGGGTC
TCAAGGTTCAGGGTCAATGCCTACTTTCAACGTACCTGTATCGCGATGGCGATTCGCGTGATTCCAACACGGATTCCCAC
ACTTGACGAACTGGACTTGCCAGCTATCATCAAAAAAATTGCTGATCGCCCACATGGACTCGTGCTGGTAACCGGTCCTA
CGGGCAGTGGAAAATCGACGACATTGGCGGCATTGCTAAATTTTATGAATCAAACACAGAGAAAACATATCGTCACGCTG
GAGGATCCGATTGAATATTTACATAAGCATGGAAACTGTATTATTGACCAGCGGGAGGTAGGGTTTGATACGGAAACCTT
TGCGAATGGATTAAGGGCAGCGTTAAGGCAGGATCCCGATGTTATTCTGGTTGGTGAGATGCGGGATTTGGAAACGATCC
AAACGGCCATTACGGCAGCTGAAACGGGGCATTTGGTTTTGGGAACATTACATACATCCAGTGCACCAGCAACGATTAAC
CGCATTATCGACGTATTCCCGCCTTCACAGCAGCCACAAATCAAAATTCAACTTGCCTCTGTTCTCGTAGCGATTGTTTC
ACAACGGCTGTTTCCAACTGCCGACAGAAAAGGAAGAAGAGCAGCTACCGAAATTTTAGTCAATAATTCTGCTGTTGCCA
ATCTGATCAGAAATGATAAAATTCATCAAATTGCCAATGTCATGCAGACTTCAAGAGCTTCAGGAATGCAAACGCTGGAA
ATGAATATTAAAGACTTACTGCAGTCAGGTGCAATCGCAAAAGAAGCAGTTGTTCCGTTCTTACAGGAAAAGGTGGAATA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.083

97.11

0.506
  pilT Vibrio cholerae strain A1552

52.083

97.11

0.506
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.121

95.376

0.497
  pilT Neisseria gonorrhoeae MS11

48.949

96.243

0.471
  pilT Neisseria meningitidis 8013

49.096

95.954

0.471
  pilT Acinetobacter baumannii D1279779

48.148

93.642

0.451
  pilT Acinetobacter nosocomialis M2

48.148

93.642

0.451
  pilT Acinetobacter baumannii strain A118

48.148

93.642

0.451
  pilT Acinetobacter baylyi ADP1

47.692

93.931

0.448
  pilT Legionella pneumophila strain ERS1305867

47.095

94.509

0.445
  pilT Legionella pneumophila strain Lp02

47.095

94.509

0.445
  pilT Pseudomonas aeruginosa PAK

48.276

92.197

0.445
  pilT Pseudomonas stutzeri DSM 10701

47.5

92.486

0.439
  pilU Pseudomonas stutzeri DSM 10701

43.293

94.798

0.41
  pilU Acinetobacter baylyi ADP1

42.006

92.197

0.387
  pilU Vibrio cholerae strain A1552

41.562

92.486

0.384