Detailed information    

insolico Bioinformatically predicted

Overview

Name   comC   Type   Machinery gene
Locus tag   RCG24_RS01150 Genome accession   NZ_CP133263
Coordinates   223211..223954 (+) Length   247 a.a.
NCBI ID   WP_308078115.1    Uniprot ID   -
Organism   Neobacillus sp. OS1-32     
Function   processing and translocation of ComGC; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218211..228954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG24_RS01125 (RCG24_01125) - 218272..218697 (+) 426 Protein_197 hypothetical protein -
  RCG24_RS01130 (RCG24_01130) - 218715..220379 (+) 1665 WP_202652285.1 GspE/PulE family protein -
  RCG24_RS01135 (RCG24_01135) pilT 220391..221431 (+) 1041 WP_202652287.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG24_RS01140 (RCG24_01140) - 221433..222638 (+) 1206 WP_202652289.1 type II secretion system F family protein -
  RCG24_RS01145 (RCG24_01145) - 222745..223209 (+) 465 WP_308078113.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG24_RS01150 (RCG24_01150) comC 223211..223954 (+) 744 WP_308078115.1 prepilin peptidase Machinery gene
  RCG24_RS01155 (RCG24_01155) pilM 223967..224974 (+) 1008 WP_308078116.1 pilus assembly protein PilM -
  RCG24_RS01160 (RCG24_01160) - 224974..225621 (+) 648 WP_202652297.1 fimbrial assembly protein -
  RCG24_RS01165 (RCG24_01165) - 225618..226373 (+) 756 WP_202652299.1 pilus assembly protein PilO -
  RCG24_RS01170 (RCG24_01170) - 226403..226852 (+) 450 WP_308078118.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RCG24_RS01175 (RCG24_01175) - 226923..228056 (+) 1134 WP_308078120.1 hypothetical protein -
  RCG24_RS01180 (RCG24_01180) - 228293..228865 (+) 573 WP_202652304.1 nucleoside triphosphate pyrophosphatase -

Sequence

Protein


Download         Length: 247 a.a.        Molecular weight: 27063.87 Da        Isoelectric Point: 9.5257

>NTDB_id=872882 RCG24_RS01150 WP_308078115.1 223211..223954(+) (comC) [Neobacillus sp. OS1-32]
MLLLLVFLYGITLGSFYNVVGLRIPVNQSIVKPRSHCPNCNHTLSAIELIPVLSYVLLRGKCRRCKAPISPFYPIVELLT
GLLFAAAPLIIGWSYELIIAWTLISLMVIVFVSDVHYMIIPDKVLIVFTLLLVAERFFLPLTPWWDSLAGAAAGFILLLV
IAIISRGGMGGGDIKLFAVIGLAVGTKLLLIAFFLSTLFGAVFGLAGMAIGKIKTGKPMAFGPYIGLGTITAYFFGERIL
QWYIDFF

Nucleotide


Download         Length: 744 bp        

>NTDB_id=872882 RCG24_RS01150 WP_308078115.1 223211..223954(+) (comC) [Neobacillus sp. OS1-32]
ATGTTGTTATTATTAGTCTTTCTCTACGGAATTACCTTAGGTTCCTTCTACAACGTAGTCGGCCTGCGGATACCAGTTAA
CCAATCCATCGTCAAACCGCGGTCGCATTGTCCAAACTGTAACCATACGTTATCAGCGATTGAGCTGATTCCAGTTTTAT
CATATGTATTATTAAGGGGGAAATGCCGGCGCTGCAAGGCGCCGATTTCGCCCTTCTATCCTATTGTCGAACTGCTGACA
GGGCTTTTGTTCGCAGCTGCTCCGCTGATCATCGGCTGGTCCTATGAACTGATCATCGCCTGGACCCTGATCTCGTTAAT
GGTGATTGTCTTTGTTTCCGATGTCCATTATATGATCATTCCTGATAAGGTTCTCATCGTCTTCACGCTGCTATTGGTAG
CAGAGAGATTCTTCCTGCCACTTACTCCATGGTGGGACTCGCTTGCCGGCGCTGCAGCCGGTTTTATCTTACTGCTTGTG
ATTGCGATCATCAGCAGAGGCGGCATGGGAGGCGGAGATATTAAACTGTTCGCCGTCATCGGACTTGCCGTTGGAACGAA
GCTCTTGCTCATCGCCTTTTTTTTGTCTACCCTTTTCGGCGCTGTGTTTGGACTAGCTGGCATGGCGATTGGCAAGATAA
AAACAGGCAAGCCGATGGCCTTTGGACCCTATATCGGCTTGGGCACCATCACCGCCTATTTCTTCGGCGAAAGAATCCTG
CAATGGTATATTGATTTTTTTTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-87)

Type IV leader peptidase family (Peptidase_A24)

(102-205)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comC Bacillus subtilis subsp. subtilis str. 168

49.18

98.785

0.486
  pilD Vibrio cholerae strain A1552

31.449

100

0.36