Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCF98_RS10650 Genome accession   NZ_CP133218
Coordinates   2185994..2187028 (-) Length   344 a.a.
NCBI ID   WP_028489670.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2180994..2192028
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS10630 (RCF98_10630) - 2181834..2182355 (-) 522 WP_028489667.1 DUF1566 domain-containing protein -
  RCF98_RS10635 (RCF98_10635) bioD 2183039..2183713 (+) 675 WP_308893679.1 dethiobiotin synthase -
  RCF98_RS10640 (RCF98_10640) - 2183696..2184826 (-) 1131 WP_028489668.1 PilT/PilU family type 4a pilus ATPase -
  RCF98_RS10645 (RCF98_10645) pilU 2184826..2185980 (-) 1155 WP_028489669.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCF98_RS10650 (RCF98_10650) pilT 2185994..2187028 (-) 1035 WP_028489670.1 type IV pilus twitching motility protein PilT Machinery gene
  RCF98_RS10655 (RCF98_10655) - 2187090..2187770 (+) 681 WP_308893683.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCF98_RS10660 (RCF98_10660) - 2187782..2188333 (-) 552 WP_028489672.1 DUF4124 domain-containing protein -
  RCF98_RS10665 (RCF98_10665) yegQ 2188438..2189793 (-) 1356 WP_028489673.1 tRNA 5-hydroxyuridine modification protein YegQ -
  RCF98_RS10670 (RCF98_10670) - 2189799..2190380 (-) 582 WP_308893686.1 thioredoxin family protein -
  RCF98_RS10675 (RCF98_10675) - 2190514..2191473 (+) 960 WP_308893688.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38199.75 Da        Isoelectric Point: 8.1677

>NTDB_id=872554 RCF98_RS10650 WP_028489670.1 2185994..2187028(-) (pilT) [Thiothrix lacustris strain MK1]
MDITQLLAFSVKNNASDLHLSGGMPPMIRVDGEMRKINLPVLDHKQVHALVYDIMNDHQRKNYEEHWECDFSFEIPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLGAPGIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYKNDTDYGHILTIED
PIEFVHESKKSLVNQREVHRDTKGFEAALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAAEKEMVRSMLSESLQAVISQTLLKKKGGGRVAAHEILIGTRAVRNLIRENKIAQMRSAMQTGQSDGMQTLDQSL
KNLVLKGLVDRETARRKAENPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=872554 RCF98_RS10650 WP_028489670.1 2185994..2187028(-) (pilT) [Thiothrix lacustris strain MK1]
ATGGATATTACCCAACTGCTGGCGTTCAGCGTCAAAAACAATGCTTCCGACTTACACTTGTCGGGGGGGATGCCACCGAT
GATCCGTGTCGATGGCGAAATGCGCAAGATCAACCTACCCGTGCTTGACCACAAACAGGTACACGCACTGGTTTACGACA
TCATGAACGATCACCAGCGCAAAAACTACGAAGAACACTGGGAATGCGATTTCTCGTTTGAAATCCCCGGCGTGGCACGT
TTCCGGGTCAATGCCTTCAACCAGAACCGTGGTGCAGGCGCGGTCTTCCGTACCATCCCCAGCAAGGTGCTGACGCTCGA
CCAACTCGGTGCTCCCGGTATTTTTCGCAAAATTTCCGAAAATCCACGCGGTTTGGTGTTAGTGACGGGACCCACAGGTT
CAGGCAAATCCACCACGCTGGCAGCAATGGTCGATTACAAAAACGATACCGATTATGGGCACATCCTCACCATTGAAGAC
CCAATCGAATTTGTCCACGAAAGCAAAAAAAGTCTGGTCAACCAACGGGAAGTCCACCGCGACACCAAAGGCTTTGAAGC
CGCCTTGCGTTCCGCCTTGCGTGAAGACCCGGACACCATTCTGGTTGGGGAAATGCGTGACCTCGAAACCATCCGCCTCG
CCCTGACCGCTGCCGAAACCGGGCATTTGGTGTTCGGCACGTTGCACACCACTTCCGCCCCCAAAACCATCGACCGTATT
GTCGACGTGTTCCCGGCAGCGGAAAAGGAAATGGTGCGTTCGATGCTTTCCGAATCCCTGCAAGCGGTCATCTCTCAAAC
CCTGCTGAAGAAAAAAGGCGGCGGACGGGTAGCAGCACACGAAATCCTCATCGGCACACGCGCAGTACGTAACCTGATCC
GCGAAAACAAAATTGCACAAATGCGCTCCGCAATGCAAACCGGGCAAAGTGATGGAATGCAAACCCTTGACCAGTCACTC
AAAAATCTGGTGTTGAAAGGGCTAGTCGATCGGGAAACCGCCCGCCGTAAAGCTGAAAACCCGGACTCCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.651

100

0.797
  pilT Pseudomonas aeruginosa PAK

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

78.198

100

0.782
  pilT Acinetobacter baumannii strain A118

78.198

100

0.782
  pilT Acinetobacter nosocomialis M2

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.744

100

0.767
  pilT Legionella pneumophila strain Lp02

72.353

98.837

0.715
  pilT Legionella pneumophila strain ERS1305867

72.353

98.837

0.715
  pilT Neisseria meningitidis 8013

71.304

100

0.715
  pilT Neisseria gonorrhoeae MS11

71.014

100

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.847

98.547

0.669
  pilT Vibrio cholerae strain A1552

67.847

98.547

0.669
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

40.29

100

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395