Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RCF98_RS10645 Genome accession   NZ_CP133218
Coordinates   2184826..2185980 (-) Length   384 a.a.
NCBI ID   WP_028489669.1    Uniprot ID   -
Organism   Thiothrix lacustris strain MK1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2179826..2190980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF98_RS10625 (RCF98_10625) - 2180352..2181842 (+) 1491 WP_308893677.1 NADH-quinone oxidoreductase subunit M -
  RCF98_RS10630 (RCF98_10630) - 2181834..2182355 (-) 522 WP_028489667.1 DUF1566 domain-containing protein -
  RCF98_RS10635 (RCF98_10635) bioD 2183039..2183713 (+) 675 WP_308893679.1 dethiobiotin synthase -
  RCF98_RS10640 (RCF98_10640) - 2183696..2184826 (-) 1131 WP_028489668.1 PilT/PilU family type 4a pilus ATPase -
  RCF98_RS10645 (RCF98_10645) pilU 2184826..2185980 (-) 1155 WP_028489669.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCF98_RS10650 (RCF98_10650) pilT 2185994..2187028 (-) 1035 WP_028489670.1 type IV pilus twitching motility protein PilT Machinery gene
  RCF98_RS10655 (RCF98_10655) - 2187090..2187770 (+) 681 WP_308893683.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCF98_RS10660 (RCF98_10660) - 2187782..2188333 (-) 552 WP_028489672.1 DUF4124 domain-containing protein -
  RCF98_RS10665 (RCF98_10665) yegQ 2188438..2189793 (-) 1356 WP_028489673.1 tRNA 5-hydroxyuridine modification protein YegQ -
  RCF98_RS10670 (RCF98_10670) - 2189799..2190380 (-) 582 WP_308893686.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 384 a.a.        Molecular weight: 42942.57 Da        Isoelectric Point: 7.5116

>NTDB_id=872553 RCF98_RS10645 WP_028489669.1 2184826..2185980(-) (pilU) [Thiothrix lacustris strain MK1]
MDRDAAVSYVHKLLATMLEKNASDLYITADAAPSAKIASEMLPLTTQALNEQNARMLVRAIMNDRQLKQFEEEMECNFSI
SLPGKARFRVNAFTQRGCAGMVLRHIPSHIPGLKDLGLPPVLRDIAMTKRGLVIMVGATGSGKSTTLASMVDFRNTNSKG
HIITIEDPIEFVHQHKGCLITQREIGVDTANYEIAMKNTLRQAPDVILLGEIRDRESMDYAIAYAETGHLCLTTLHANSS
NQAIDRIINFFPEERRNQLLMDLSLNLKAVVSQRLVRKAKGEGRLAAVEVMINTPLMADLILKGDVPGMKTLTSKSREHG
MRTFDQALFDLIESRQITVKEALRHADSQNDLRLRLKLESRFAQDNDFFKGLDDLAIEPMENAR

Nucleotide


Download         Length: 1155 bp        

>NTDB_id=872553 RCF98_RS10645 WP_028489669.1 2184826..2185980(-) (pilU) [Thiothrix lacustris strain MK1]
ATGGATCGCGATGCCGCCGTCAGCTATGTCCACAAATTGCTGGCCACCATGTTGGAAAAAAACGCTTCCGACTTGTATAT
CACGGCGGATGCCGCCCCCTCCGCCAAAATTGCCAGCGAAATGCTGCCACTGACCACACAAGCTTTGAATGAGCAAAATG
CCCGCATGTTGGTACGTGCCATCATGAATGACCGCCAGCTCAAACAGTTTGAGGAAGAGATGGAATGCAACTTCTCCATC
AGTCTGCCCGGCAAGGCACGTTTCCGGGTGAATGCCTTCACCCAGCGTGGTTGTGCAGGCATGGTGCTGCGGCATATTCC
CAGCCATATACCCGGCCTGAAAGACTTGGGGCTACCGCCGGTATTACGCGACATCGCCATGACCAAACGCGGGCTGGTGA
TCATGGTTGGTGCGACCGGCTCGGGTAAATCCACCACGCTGGCGTCGATGGTCGACTTCCGCAATACCAACTCCAAGGGT
CACATCATCACCATTGAAGACCCGATCGAATTTGTCCACCAGCACAAAGGCTGCCTGATTACCCAACGGGAAATCGGGGT
CGATACCGCCAATTACGAAATAGCCATGAAGAACACCCTGCGGCAAGCACCCGACGTCATCCTGCTCGGGGAAATCCGTG
ACCGCGAGTCGATGGATTACGCGATTGCCTATGCTGAAACCGGGCATCTGTGCCTGACGACCTTACACGCCAATAGCTCT
AACCAAGCCATTGACCGCATTATCAACTTTTTCCCAGAAGAACGCCGCAACCAGTTATTGATGGATTTATCCCTCAACCT
GAAAGCCGTGGTATCACAACGGCTGGTGCGCAAAGCCAAAGGCGAAGGGCGTTTAGCTGCCGTGGAAGTGATGATCAATA
CCCCGCTGATGGCTGATCTAATCCTCAAAGGCGACGTACCGGGGATGAAAACCCTCACCTCCAAGTCCCGCGAACACGGG
ATGCGTACTTTCGACCAAGCCCTATTTGATCTGATCGAATCCCGTCAGATTACCGTGAAAGAAGCCCTGCGCCATGCCGA
CTCTCAAAATGACCTACGTCTGCGCCTCAAGCTAGAAAGCCGCTTTGCCCAAGACAACGACTTTTTCAAGGGTCTGGATG
ATCTTGCTATCGAACCCATGGAGAATGCCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.599

90.885

0.542
  pilU Acinetobacter baylyi ADP1

56.484

90.365

0.51
  pilU Vibrio cholerae strain A1552

50.862

90.625

0.461
  pilT Acinetobacter nosocomialis M2

44.412

88.542

0.393
  pilT Acinetobacter baumannii D1279779

44.118

88.542

0.391
  pilT Acinetobacter baumannii strain A118

44.118

88.542

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.817

85.417

0.383
  pilT Acinetobacter baylyi ADP1

44.144

86.719

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.547

90.885

0.378
  pilT Vibrio cholerae strain A1552

41.547

90.885

0.378
  pilT Pseudomonas stutzeri DSM 10701

43.963

84.115

0.37
  pilT Legionella pneumophila strain Lp02

43.161

85.677

0.37
  pilT Legionella pneumophila strain ERS1305867

43.161

85.677

0.37
  pilT Pseudomonas aeruginosa PAK

42.342

86.719

0.367