Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RCG00_RS03510 Genome accession   NZ_CP133217
Coordinates   511716..512750 (-) Length   344 a.a.
NCBI ID   WP_308135872.1    Uniprot ID   A0AA51MN57
Organism   Thiothrix subterranea strain DNT52     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 506716..517750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCG00_RS03490 (RCG00_03490) - 507958..508476 (-) 519 WP_308872071.1 DUF1566 domain-containing protein -
  RCG00_RS03495 (RCG00_03495) bioD 508762..509421 (+) 660 WP_308872073.1 dethiobiotin synthase -
  RCG00_RS03500 (RCG00_03500) - 509418..510548 (-) 1131 WP_308872074.1 PilT/PilU family type 4a pilus ATPase -
  RCG00_RS03505 (RCG00_03505) pilU 510545..511702 (-) 1158 WP_308872075.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCG00_RS03510 (RCG00_03510) pilT 511716..512750 (-) 1035 WP_308135872.1 type IV pilus twitching motility protein PilT Machinery gene
  RCG00_RS03515 (RCG00_03515) - 512812..513498 (+) 687 WP_308872077.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCG00_RS03520 (RCG00_03520) - 513505..514002 (-) 498 WP_308872079.1 DUF4124 domain-containing protein -
  RCG00_RS03525 (RCG00_03525) - 514334..515227 (+) 894 WP_308135869.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38200.75 Da        Isoelectric Point: 8.1677

>NTDB_id=872520 RCG00_RS03510 WP_308135872.1 511716..512750(-) (pilT) [Thiothrix subterranea strain DNT52]
MDITQLLAFSVKNNASDLHLSGGMPPMIRVDGEMRKINLPVLDHKQVHALVYDIMNDHQRKNYEEHWECDFSFEIPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTLDQLGAPGIFRKISENPRGLVLVTGPTGSGKSTTLAAMVDYKNDTDYGHILTIED
PIEFVHESKKSLVNQREVHRDTKGFEAALRSALREDPDTILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPAAEKEMVRSMLSESLQAVISQTLLKKKGGGRVAAHEILIGTRAVRNLIRENKIAQMRSAMQTGQNDGMQTLDQSL
KNLVMKGLVDREAARRKADNPDSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=872520 RCG00_RS03510 WP_308135872.1 511716..512750(-) (pilT) [Thiothrix subterranea strain DNT52]
ATGGACATTACCCAACTGCTGGCGTTTAGCGTCAAAAACAATGCCTCCGACTTACACTTGTCGGGCGGGATGCCCCCCAT
GATCCGTGTCGATGGCGAAATGCGCAAGATCAACTTGCCGGTACTCGACCATAAACAGGTACACGCGCTGGTGTACGACA
TCATGAATGATCACCAACGTAAAAACTACGAAGAGCATTGGGAATGCGATTTCTCGTTTGAAATCCCTGGTGTGGCGCGG
TTTCGTGTGAATGCCTTTAACCAAAACCGTGGTGCGGGTGCGGTGTTCCGTACTATCCCTAGCAAAGTATTAACACTCGA
TCAGTTGGGTGCGCCCGGTATTTTTCGGAAAATTTCGGAAAACCCACGCGGCTTGGTGTTGGTAACAGGGCCGACCGGTT
CGGGCAAATCCACCACGCTTGCGGCAATGGTCGATTACAAAAATGATACCGATTACGGGCATATTCTAACCATCGAAGAC
CCGATCGAATTCGTGCACGAAAGCAAAAAAAGCTTGGTCAACCAACGTGAAGTGCACCGCGATACCAAGGGTTTTGAAGC
CGCGTTACGTTCCGCGTTACGTGAAGACCCGGATACCATTCTGGTCGGGGAAATGCGTGACTTGGAAACGATTCGCTTGG
CATTGACGGCGGCAGAAACCGGGCATTTGGTGTTTGGTACATTGCATACGACATCCGCACCCAAAACCATTGACCGTATT
GTGGATGTGTTCCCCGCTGCCGAAAAAGAGATGGTGCGTTCCATGCTTTCCGAATCCTTGCAGGCGGTGATTTCACAGAC
CCTGCTGAAGAAAAAAGGCGGCGGGCGTGTGGCTGCACACGAAATTCTGATTGGCACGCGAGCGGTACGCAATTTAATCC
GCGAAAATAAGATTGCGCAAATGCGTTCCGCGATGCAAACCGGGCAAAATGACGGAATGCAAACCCTTGATCAATCATTG
AAAAATTTGGTGATGAAAGGGTTGGTGGATCGTGAAGCGGCGCGGCGGAAAGCGGATAACCCGGATTCTATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

79.36

100

0.794
  pilT Pseudomonas aeruginosa PAK

79.36

100

0.794
  pilT Acinetobacter nosocomialis M2

78.198

100

0.782
  pilT Acinetobacter baumannii D1279779

78.198

100

0.782
  pilT Acinetobacter baumannii strain A118

78.198

100

0.782
  pilT Acinetobacter baylyi ADP1

76.453

100

0.765
  pilT Neisseria gonorrhoeae MS11

71.304

100

0.715
  pilT Neisseria meningitidis 8013

71.304

100

0.715
  pilT Legionella pneumophila strain Lp02

72.059

98.837

0.712
  pilT Legionella pneumophila strain ERS1305867

72.059

98.837

0.712
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.552

98.547

0.666
  pilT Vibrio cholerae strain A1552

67.552

98.547

0.666
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.462

99.419

0.512
  pilU Vibrio cholerae strain A1552

40.87

100

0.41
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398